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bcl 2 coding sequence  (Addgene inc)


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    Structured Review

    Addgene inc bcl 2 coding sequence
    Bcl 2 Coding Sequence, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coding+sequence+of+bcl-2/pCEP4+Bcl-2+(Plasmid+%2316461)/pmc09586935-345-0-6
    Average 93 stars, based on 7 article reviews
    bcl 2 coding sequence - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Over Expression:

    Article Title: AS1411-Induced Growth Inhibition of Glioma Cells by Up-Regulation of p53 and Down-Regulation of Bcl-2 and Akt1 via Nucleolin
    Article Snippet: The scrambled siRNA used as a negative control was from Ambion. .. For the gene overexpression, pCEP4 Bcl-2 (Plasmid #16461), pCDH-puro-myr-HA-Akt1 (Plasmid #46969) and GFP-Nucleolin (Plasmid #28176) were obtained from the Addgene (Cambridge, MA). ..

    Plasmid Preparation:

    Article Title: AS1411-Induced Growth Inhibition of Glioma Cells by Up-Regulation of p53 and Down-Regulation of Bcl-2 and Akt1 via Nucleolin
    Article Snippet: The scrambled siRNA used as a negative control was from Ambion. .. For the gene overexpression, pCEP4 Bcl-2 (Plasmid #16461), pCDH-puro-myr-HA-Akt1 (Plasmid #46969) and GFP-Nucleolin (Plasmid #28176) were obtained from the Addgene (Cambridge, MA). ..

    Article Title: Method for inhibition of deubiquitinating activity
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (26) and isolating high expression clones. ..

    Article Title: Protease deubiquitinating inhibitor screening
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (37) and isolating high expression clones. ..

    Article Title: Inactivation of Bcl-2 through IκB kinase (IKK)-dependent phosphorylation mediates apoptosis upon exposure to 4-hydroxynonenal (HNE).
    Article Snippet: Apoptosis of macrophage foam cells loaded with modified/oxidized lipids is implicated in destabilization of advanced atherosclerotic plaques in humans.. Concentration of HNE, main aldehydic product of plasma LDL peroxidation, elevates in atherosclerotic lesions as well as in cultured cells under oxidative stress.. Although this reactive aldehyde has been shown to promote apoptosis with the involvement of p38 MAPK and JNK in various mammalian cell lines, roles of B-cell lymphoma 2 (Bcl-2) family proteins remain to be deciphered.

    Article Title: MiR-424 and miR-27a increase TRAIL sensitivity of acute myeloid leukemia by targeting PLAG1
    Article Snippet: .. Similarly the ORF of Bcl2 cloned from Bcl2-pCEP4 (Addgene, Plasmid# 16461) was inserted into pLenti-C-GFP vector. ..

    Generated:

    Article Title: Method for inhibition of deubiquitinating activity
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (26) and isolating high expression clones. ..

    Article Title: Protease deubiquitinating inhibitor screening
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (37) and isolating high expression clones. ..

    Expressing:

    Article Title: Method for inhibition of deubiquitinating activity
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (26) and isolating high expression clones. ..

    Article Title: Protease deubiquitinating inhibitor screening
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (37) and isolating high expression clones. ..

    Clone Assay:

    Article Title: Method for inhibition of deubiquitinating activity
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (26) and isolating high expression clones. ..

    Article Title: Protease deubiquitinating inhibitor screening
    Article Snippet: .. The HCT-116 Bcl-2+/+ cell line was generated by transfecting parental HCT-116 p53+/+ cells with pCEP4 Bcl-2 (Addgene plasmid 16461) (37) and isolating high expression clones. ..

    Article Title: MiR-424 and miR-27a increase TRAIL sensitivity of acute myeloid leukemia by targeting PLAG1
    Article Snippet: .. Similarly the ORF of Bcl2 cloned from Bcl2-pCEP4 (Addgene, Plasmid# 16461) was inserted into pLenti-C-GFP vector. ..

    Transfection:

    Article Title: Inactivation of Bcl-2 through IκB kinase (IKK)-dependent phosphorylation mediates apoptosis upon exposure to 4-hydroxynonenal (HNE).
    Article Snippet: Apoptosis of macrophage foam cells loaded with modified/oxidized lipids is implicated in destabilization of advanced atherosclerotic plaques in humans.. Concentration of HNE, main aldehydic product of plasma LDL peroxidation, elevates in atherosclerotic lesions as well as in cultured cells under oxidative stress.. Although this reactive aldehyde has been shown to promote apoptosis with the involvement of p38 MAPK and JNK in various mammalian cell lines, roles of B-cell lymphoma 2 (Bcl-2) family proteins remain to be deciphered.

    Article Title: The accumulation of miR-125b-5p is indispensable for efficient erythroblast enucleation
    Article Snippet: .. Bcl-2 coding sequence (pCEP4 Bcl-2, #16461, Addgene, Cambridge, MA, USA) was transfected and selected with 50 μg mL −1 hygromycin B. .. According to the manufacturer’s instructions, cells were seeded in 6-well plates and transfected with 3 μg per well miRNA mimics (Genepharma, Shanghai, China) or non-target NC mimics (Table ) with the aid of Entranster TM -R4000 (Engreen).

    Article Title: The accumulation of miR-125b-5p is indispensable for efficient erythroblast enucleation.
    Article Snippet: .. Bcl-2 coding sequence (pCEP4 Bcl-2, #16461, Addgene, Cambridge, MA, USA) was transfected and selected with 50 μgmL−1 hygromycin B. miRNA mimic and inhibitor transfection According to the manufacturer’s instructions, cells were seeded in 6-well plates and transfected with 3 μg per well miRNA mimics (Genepharma, Shanghai, China) or non-target NC mimics (Table S2) with the aid of EntransterTM-R4000 (Engreen). .. K562-pc125b cells or K562-pc3 cells (6 × 104) were co-transfected with 8 ng pRL Renilla luciferase vector (Promega, Madison, WI, USA) as internal control and 800 ng pGL3-Bcl-2-3'UTR or pGL3 vector control.

    Sequencing:

    Article Title: The accumulation of miR-125b-5p is indispensable for efficient erythroblast enucleation
    Article Snippet: .. Bcl-2 coding sequence (pCEP4 Bcl-2, #16461, Addgene, Cambridge, MA, USA) was transfected and selected with 50 μg mL −1 hygromycin B. .. According to the manufacturer’s instructions, cells were seeded in 6-well plates and transfected with 3 μg per well miRNA mimics (Genepharma, Shanghai, China) or non-target NC mimics (Table ) with the aid of Entranster TM -R4000 (Engreen).

    Article Title: The accumulation of miR-125b-5p is indispensable for efficient erythroblast enucleation.
    Article Snippet: .. Bcl-2 coding sequence (pCEP4 Bcl-2, #16461, Addgene, Cambridge, MA, USA) was transfected and selected with 50 μgmL−1 hygromycin B. miRNA mimic and inhibitor transfection According to the manufacturer’s instructions, cells were seeded in 6-well plates and transfected with 3 μg per well miRNA mimics (Genepharma, Shanghai, China) or non-target NC mimics (Table S2) with the aid of EntransterTM-R4000 (Engreen). .. K562-pc125b cells or K562-pc3 cells (6 × 104) were co-transfected with 8 ng pRL Renilla luciferase vector (Promega, Madison, WI, USA) as internal control and 800 ng pGL3-Bcl-2-3'UTR or pGL3 vector control.



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