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150 mm sodium phosphate, ph 8.5  (Microarrays Inc)

 
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    Microarrays Inc 150 mm sodium phosphate, ph 8.5
    150 Mm Sodium Phosphate, Ph 8.5, supplied by Microarrays Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/codelink+activated+microarray+slides/150+mm+sodium+phosphate++ph+8+5/pm18757798-83-7-11
    Average 90 stars, based on 1 article reviews
    150 mm sodium phosphate, ph 8.5 - by Bioz Stars, 2026-09
    90/100 stars

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    Concentration Assay:

    Article Title: Transcriptional interplay among the regulators Rrp2, RpoN and RpoS in Borrelia burgdorferi.
    Article Snippet: .. The oligonucleotides were resuspended in 150 mM sodium phosphate, pH 8.5 (Microarrays Inc.), to a concentration of 40 mM and printed on CodeLink activated slides (Amersham Biosciences), using a Custom arrayer (Microarrays Inc.). ..



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    Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

    Journal: PLoS Pathogens

    Article Title: Genome-Wide Fitness Test and Mechanism-of-Action Studies of Inhibitory Compounds in Candida albicans

    doi: 10.1371/journal.ppat.0030092

    Figure Lengend Snippet: Individual heterozygous deletion strains contain two unique barcodes, up-tags (red) and down-tags (blue), flanked by two pairs of common primer sequences (black for all the up-tags, grey for all the down-tags) at the deleted allele. The CaFT pool contains 2,868 strains, representing ∼45% of the C. albicans genome. Aliquots of the pool are treated with an inhibitory compound (at different concentrations) or a mock treatment over 20 population doublings. The relative abundance of each strain is subsequently monitored by DNA microarrays competitively hybridized with amplified and labeled tags (using the common primer pairs) from the two treatments. The response of each strain to the compound is appraised by a normalized z -score, with a positive value indicating hypersensitivity, and a negative value relative resistance.

    Article Snippet: We custom-built a set of two DNA microarrays using Amersham CodeLink Activated Slides ( http://www.gelifesciences.com/ ).

    Techniques: Amplification, Labeling