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mouse osteocalcin elisa antibody coating buffer  (ImmunoChemistry Technologies)


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    Structured Review

    ImmunoChemistry Technologies mouse osteocalcin elisa antibody coating buffer
    (A) The specificity of the different goat antibodies were tested by western blotting on dot blot membranes. GLA-OCN: fully carboxylated <t>osteocalcin.</t> GLU-OCN: uncarboxylated osteocalcin.
    Mouse Osteocalcin Elisa Antibody Coating Buffer, supplied by ImmunoChemistry Technologies, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/coating+buffer/Antibody+Coating+Buffer/pmc02913292-59-4-33
    Average 90 stars, based on 7 article reviews
    mouse osteocalcin elisa antibody coating buffer - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "AN ELISA-BASED METHOD TO QUANTIFY OSTEOCALCIN CARBOXYLATION IN MICE"

    Article Title: AN ELISA-BASED METHOD TO QUANTIFY OSTEOCALCIN CARBOXYLATION IN MICE

    Journal:

    doi: 10.1016/j.bbrc.2010.06.008

    (A) The specificity of the different goat antibodies were tested by western blotting on dot blot membranes. GLA-OCN: fully carboxylated osteocalcin. GLU-OCN: uncarboxylated osteocalcin.
    Figure Legend Snippet: (A) The specificity of the different goat antibodies were tested by western blotting on dot blot membranes. GLA-OCN: fully carboxylated osteocalcin. GLU-OCN: uncarboxylated osteocalcin.

    Techniques Used: Western Blot, Dot Blot

    (A) Quantitative measurement of GLU-OCN, GLA13-OCN and total osteocalcin in the supernatant of primary osteoblasts cultures (pOB) treated with a vehicle or with warfarin. *** p < 0.001 compared to vehicle treated osteoblasts.
    Figure Legend Snippet: (A) Quantitative measurement of GLU-OCN, GLA13-OCN and total osteocalcin in the supernatant of primary osteoblasts cultures (pOB) treated with a vehicle or with warfarin. *** p < 0.001 compared to vehicle treated osteoblasts.

    Techniques Used:

    (A) Quantitative measurement of GLU-OCN, GLA13-OCN and total osteocalcin in serums from 2 months old wild type (WT) and Ocn −/− mice. All three forms were undetectable (UN) in Ocn −/− serums.
    Figure Legend Snippet: (A) Quantitative measurement of GLU-OCN, GLA13-OCN and total osteocalcin in serums from 2 months old wild type (WT) and Ocn −/− mice. All three forms were undetectable (UN) in Ocn −/− serums.

    Techniques Used:

    Related Articles

    Blocking Assay:

    Article Title: Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin and Its Antithrombotic Activity
    Article Snippet: Cholesterol was from Sigma-Aldrich (St. Louis, USA). .. Antibody coating buffer, Neptune block, Neptune sample diluent, ELISA wash buffer, TMB 1-component HRP microwell substrate (SUBT) and stop solution for TMB substrates (STOPT) were from ImmunoChemistry Technologies, LLC (Bloomington, MN). .. The mouse anti-TM monoclonal antibody PBS-01 was from Abcam (Cambridge, MA).

    Article Title: Embryonic osteocalcin signaling determines lifelong adrenal steroidogenesis and homeostasis in the mouse
    Article Snippet: .. Plates were coated with either anti-GLA or anti-MID antibodies in Antibody Coating Buffer (ImmunoChemistry Technologies) for 12 hours at room temperature and then washed once (0.1% Tween-20 in 1× PBS) and coated with blocking buffer (3% BSA and 0.1% Tween-20 in 1× PBS) for 4 hours. ..

    Article Title: Embryonic osteocalcin signaling determines lifelong adrenal steroidogenesis and homeostasis in the mouse
    Article Snippet: .. Plates were coated with either anti-GLA or anti-MID antibodies in Antibody Coating Buffer (ImmunoChemistry Technologies) for 12 hours at room temperature and then washed once (0.1% Tween-20 in 1× PBS) and coated with blocking buffer (3% BSA and 0.1% Tween-20 in 1× PBS) for 4 hours. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Recombinant and Chemo-/Bio-Orthogonal Synthesis of Liposomal Thrombomodulin and Its Antithrombotic Activity
    Article Snippet: Cholesterol was from Sigma-Aldrich (St. Louis, USA). .. Antibody coating buffer, Neptune block, Neptune sample diluent, ELISA wash buffer, TMB 1-component HRP microwell substrate (SUBT) and stop solution for TMB substrates (STOPT) were from ImmunoChemistry Technologies, LLC (Bloomington, MN). .. The mouse anti-TM monoclonal antibody PBS-01 was from Abcam (Cambridge, MA).

    Article Title: Metabolic phenotype in the mouse model of osteogenesis imperfecta
    Article Snippet: .. Briefly, Antibody Coating Buffer (CB1), ELISA Wash Buffer (WB1), 120 General Blocker Buffer (BB1), General Assay Diluent (AD1) and Stop Solution for TMB 121 (STOP1) were all obtained from ImmunoChemistry Technologies. .. 1-StepTM Ultra TMB ELISA 122 substrate was from Pierce.

    Article Title: Targeting Bone Cells During Sexual Maturation Reveals Sexually Dimorphic Regulation of Endochondral Ossification
    Article Snippet: .. Carboxylated (Gla) and total mouse OCN were measured in serum collected before and 1 or 2 weeks after administration of DT, using two specific ELISA assays as has been previously described. ( ) Briefly, 96‐well ELISA plates (R&D Systems, Minneapolis, MN, USA) were coated with 100 μL of anti‐Gla‐OCN (2 μg/mL) or anti‐Mid‐OCN (1.5 μg/mL) diluted in 1X antibody coating buffer (Immunochemistry Technologies, Bloomington, MN, USA) and incubated overnight at room temperature. ..

    Incubation:

    Article Title: Targeting Bone Cells During Sexual Maturation Reveals Sexually Dimorphic Regulation of Endochondral Ossification
    Article Snippet: .. Carboxylated (Gla) and total mouse OCN were measured in serum collected before and 1 or 2 weeks after administration of DT, using two specific ELISA assays as has been previously described. ( ) Briefly, 96‐well ELISA plates (R&D Systems, Minneapolis, MN, USA) were coated with 100 μL of anti‐Gla‐OCN (2 μg/mL) or anti‐Mid‐OCN (1.5 μg/mL) diluted in 1X antibody coating buffer (Immunochemistry Technologies, Bloomington, MN, USA) and incubated overnight at room temperature. ..



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