mtcoii (Proteintech)

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Proteintech mtcoii
Mtcoii, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 215 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/co+2/MTCO2+Polyclonal+antibody/pm33785595-243-70-71
Average 96 stars, based on 215 article reviews

mtcoii - by Bioz Stars, 2026-11

96/100 stars

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Immunohistochemical staining:

Article Title: Immunohistochemical Markers of Mitochondrial Electron Transport Chain Instability in Human Brain Regions: A Study of Aging and Alzheimer’s Disease
Article Snippet: .. Immunohistochemical analysis was performed using a panel of four primary antibodies: ATP5H Monoclonal Antibody (7F9BG1); monoclonal; ThermoFisher (Waltham, MA, USA); 459000; dilution 1:200; MTCO2 Polyclonal antibody; polyclonal; Proteintech (Rosemont, IL, USA); 55070-1-AP; dilution 1:200; MTCO1 Monoclonal Antibody (1D6E1A8); monoclonal; ThermoFisher (Waltham, MA, USA); 459600; dilution 1:200; ATPIF1 Monoclonal Antibody (5E2D7); monoclonal; ThermoFisher (Waltham, MA, USA); A-21355; dilution 1:200. .. Antibody detection was achieved using the Novolink Polymer Detection System (Leica Biosystems, Nussloch, Germany), a polymer-based peroxidase visualization kit.

Incubation:

Article Title: Icariin synergistically inhibits glycolysis and mitochondrial energy metabolism in lung cancer in association with the HIF-1α/LONP1 axis.
Article Snippet: Aim of the study: Non-small cell lung cancer (NSCLC) accounts for the vast majority of lung cancer cases and remains a therapeutic challenge due to drug resistance, adverse effects, and metabolic plasticity.. Icariin (ICR), a principal bioactive flavonoid from Epimedium, exhibits promising antitumor properties; however, its precise role in modulating cancer energy metabolism remains poorly understood.. This study aimed to investigate whether ICR synergistically inhibits glycolysis and mitochondrial metabolism, and whether these effects are associated with the HIF-1α/LONP1 signaling axis in NSCLC.

Cell-Signaling:

Article Title: METTL1-mediated m 7 G modification of valine tRNAs drives metabolic adaptation in pancreatic ductal adenocarcinoma cells
Article Snippet: The immunoblots were subsequently exposed to an HRP-based chemiluminescence kit (Thermo Fisher Scientific), and the signal was imaged using an ECL device (Bio-Rad). .. The primary antibodies used in this study included anti–β-actin (Proteintech, 66009), anti-METTL1 (Proteintech, 14994-1-AP), and anti-puromycin (Abcam, 315887), CD44 (Cell Signaling Technology, 3570), calnexin (Cell Signaling Technology, 2679), MT-ND1(Proteintech, 19703-1-AP), and MT-CO2 (Proteintech, 55070-1-AP). ..

Western Blot:

Article Title: Copper depletion inhibits electron transport chain activity and nucleotide synthesis to boost leukemia therapy
Article Snippet: .. Immunoblotting was performed using the following primary antibodies: For reducing and denaturing western blot: vinculin (CST 13901; 1:2500) Beta actin (CST 8457; 1:2000) GAPDH (CST 2118L; 1:2000) MT-CO1/COX1 (abcam ab14705; 1:2000) COX4I1/COX4 (CST 4850; 1:1000) COX2 (Proteintech 55070–1-AP; 1:5000) DHODH (Santa Cruz E-8 sc-166348; 1:500) SDHA (CST 5839S; 1:1000) SLC31A1 (CST 13086S; 1:500) Beta tubulin (CST 2128P; 1:1000) Human Total OxPhos Cocktail (abcam ab110411; 1:1000) VDAC1/porin (abcam ab154856; 1:1000) Total ERK1/2 (CST 4696S; 1:1000) Phospho-ERK1/2 (CST 4370L; 1:1000) Primary antibodies were diluted in Signal Enhancer HIKARI Solution 1 (Nacalai USA NU00102). .. For blue-native PAGE, the following antibodies were used: MT-CO1/COX1 (ABCAM, ab14705, 1:2000) SDHA (Santa Cruz, sc-166947,1:20,000) NDUFS1 (Abcam, ab169540,1:1000) UQCR4/CYC1 (Millipore Sigma, HPA001247,1:2000) Secondary antibodies used were: Peroxidase AffiniPure Goat Anti-Mouse IgG H+L (Jackson ImmunoResearch Laboratories, #115–035-166) and Peroxidase AffiniPure Goat Anti-Rabbit IgG H+L (Jackson ImmunoResearch Laboratories, #111–035-144) were both diluted 1:10,000 in 5% milk in TBST and incubated with immunoblots for 1 hour at room temperature.

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