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cloud sequencing technology  (10X Genomics)

 
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    10X Genomics cloud sequencing technology
    The five different enzyme concentrations/ng DNA using stLFR <t>sequencing</t> of D. wulumuqiensis R12 (A) The barcode frequency distribution of five conditions. (B) The Supernova-assembled statistics of five conditions.
    Cloud Sequencing Technology, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloud+sequencing+technology/libraries+sequencing/pmc07961107-133-36-33
    Average 86 stars, based on 1 article reviews
    cloud sequencing technology - by Bioz Stars, 2026-09
    86/100 stars

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    1) Product Images from "Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content"

    Article Title: Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content

    Journal: iScience

    doi: 10.1016/j.isci.2021.102219

    The five different enzyme concentrations/ng DNA using stLFR sequencing of D. wulumuqiensis R12 (A) The barcode frequency distribution of five conditions. (B) The Supernova-assembled statistics of five conditions.
    Figure Legend Snippet: The five different enzyme concentrations/ng DNA using stLFR sequencing of D. wulumuqiensis R12 (A) The barcode frequency distribution of five conditions. (B) The Supernova-assembled statistics of five conditions.

    Techniques Used: Sequencing

    Statistics of three sequencing strategies assembly genomes
    Figure Legend Snippet: Statistics of three sequencing strategies assembly genomes

    Techniques Used: Sequencing

    The three sequencing strategies-assembled genome statistics of the six bacterial strains The total assembly genome length (bottom), N50 length (middle), and maximum scaffold length (top) by using different algorithms are shown. The assembly algorithms of each sample from left to right are NGS draft genome, stLFR chromosome scaffolds, stLFR + ONT complete genome, ONT complete genome assembled by Canu, and Hybrid complete genome assembled by Unicycler using ONT reads and NGS reads.
    Figure Legend Snippet: The three sequencing strategies-assembled genome statistics of the six bacterial strains The total assembly genome length (bottom), N50 length (middle), and maximum scaffold length (top) by using different algorithms are shown. The assembly algorithms of each sample from left to right are NGS draft genome, stLFR chromosome scaffolds, stLFR + ONT complete genome, ONT complete genome assembled by Canu, and Hybrid complete genome assembled by Unicycler using ONT reads and NGS reads.

    Techniques Used: Sequencing

    The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation sequencing-assembled genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.
    Figure Legend Snippet: The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation sequencing-assembled genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.

    Techniques Used: Sequencing

    The longest chromosome sequence comparison (A and B) (A) Sequences alignment viewer and (B) accuracy evaluation of stLFR genomes, stLFR + ONT genomes, Canu-assembled genomes, and NGS genomes when compared with the complete genomes assembled by Unicycler using NGS and ONT reads for the strains E. coli K-12, Rufibacter sp. LB8, D. wulumuqiensis R12, and J. melonis M714.
    Figure Legend Snippet: The longest chromosome sequence comparison (A and B) (A) Sequences alignment viewer and (B) accuracy evaluation of stLFR genomes, stLFR + ONT genomes, Canu-assembled genomes, and NGS genomes when compared with the complete genomes assembled by Unicycler using NGS and ONT reads for the strains E. coli K-12, Rufibacter sp. LB8, D. wulumuqiensis R12, and J. melonis M714.

    Techniques Used: Sequencing, Comparison

    Related Articles

    DNA Sequencing:

    Article Title: The chromosome-level genome sequences of Danio rerio strains AB, Nadia and Cooch Behar
    Article Snippet: .. 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers’ instructions. .. Sequencing was carried out at the Scientific Operations Core at the Wellcome Sanger Institute on an Illumina HiSeq X Ten instrument.

    Article Title: The genome sequence of the Dusky Thorn moth, Ennomos fuscantarius (Haworth, 1809)
    Article Snippet: Analysis of the integrity of the RNA was done using Agilent RNA 6000 Pico Kit and Eukaryotic Total RNA assay. .. Pacific Biosciences HiFi circular consensus and 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers’ instructions. .. Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit.

    Article Title: The genome sequence of the Buff Arches, Habrosyne pyritoides (Hufnagel, 1766)
    Article Snippet: Analysis of the integrity of the RNA was done using Agilent RNA 6000 Pico Kit and Eukaryotic Total RNA assay. .. Pacific Biosciences HiFi circular consensus and 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers’ instructions. .. Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit.

    Construct:

    Article Title: The chromosome-level genome sequences of Danio rerio strains AB, Nadia and Cooch Behar
    Article Snippet: .. 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers’ instructions. .. Sequencing was carried out at the Scientific Operations Core at the Wellcome Sanger Institute on an Illumina HiSeq X Ten instrument.

    Article Title: The genome sequence of the Dusky Thorn moth, Ennomos fuscantarius (Haworth, 1809)
    Article Snippet: Analysis of the integrity of the RNA was done using Agilent RNA 6000 Pico Kit and Eukaryotic Total RNA assay. .. Pacific Biosciences HiFi circular consensus and 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers’ instructions. .. Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit.

    Article Title: The genome sequence of the Buff Arches, Habrosyne pyritoides (Hufnagel, 1766)
    Article Snippet: Analysis of the integrity of the RNA was done using Agilent RNA 6000 Pico Kit and Eukaryotic Total RNA assay. .. Pacific Biosciences HiFi circular consensus and 10X Genomics read cloud DNA sequencing libraries were constructed according to the manufacturers’ instructions. .. Poly(A) RNA-Seq libraries were constructed using the NEB Ultra II RNA Library Prep kit.

    Article Title: Monocyte/macrophage-derived NLRP3 Promotes the Onset and Progression of Ankylosing Spondylitis Via the NOD-like Receptor Pathway.
    Article Snippet: .. After cell isolation and extraction, singlecell sequencing libraries were constructed using the 10X Genomics platform [11] and sequencing was subsequently performed. ..

    Sequencing:

    Article Title: Multiomics and multi-region spatial transcriptome analysis reveal cellular networks and pathways associated with HCC recurrence.
    Article Snippet: The quantity and quality of the cDNA were determined using an Agilent Bioanalyzer High-Sensitivity Chip, and the cDNA was diluted tenfold prior to loading onto the chip. .. Illumina sequencing library generation The sequencing library was prepared according to the protocol provided by 10X Genomics. ..

    Article Title: An updated and spatially validated somatic single-cell atlas of Hydractinia symbiolongicarpus
    Article Snippet: Cells were thawed, spun, and resuspended but cell counts were not obtained again, and the samples were loaded into the 10X Genomics platform for encapsulation with the capture target of 3,000–9,000 cells per sample (Supplementary Table ). .. Sequencing libraries were prepared according to the standard 10X Genomics V3 chemistry protocol. .. The cDNA libraries were pooled and sequenced as 150 bp paired end reads and single indexed on an Illumina NovaSeq6000 with 63 million projected clusters per sample.

    Article Title: Monocyte/macrophage-derived NLRP3 Promotes the Onset and Progression of Ankylosing Spondylitis Via the NOD-like Receptor Pathway.
    Article Snippet: .. After cell isolation and extraction, singlecell sequencing libraries were constructed using the 10X Genomics platform [11] and sequencing was subsequently performed. ..

    Article Title: Single-cell transcriptomics identifies regulatory T cell heterogeneity in gestational diabetes mellitus.
    Article Snippet: .. Single-cell mRNA, dual-indexed, sequencing libraries were generated following the Chromium Single Cell 5’ v2 protocol with Feature Barcode technology for Cell Surface Protein and Immune Receptor Mapping (CG000330, 10x Genomics). .. For each 10x run, patient samples were hash-tagged and pooled, using one antibody-derived tag per patient following the CITE-Seq protocol 55 (Biolegend) and then flowsorted to generate separate compartments of CD4+ T-cells and CD25+CD127lo T-cells before proceeding with the 10x assay.

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
    Article Snippet: Individual nuclei were then coupled to beads using the 10X Genomics Chromium controller. .. Single nuclei sequencing libraries were prepared following the 10X Genomics Chromium GEM-X Single Cell 3 ′ V4 kit protocol. .. Libraries were paired end sequenced on an Illumina NovaSeq X Plus platform (28 × 10 × 10X90X) to a minimum depth of 100,000 reads per nucleus.

    Cell Isolation:

    Article Title: Monocyte/macrophage-derived NLRP3 Promotes the Onset and Progression of Ankylosing Spondylitis Via the NOD-like Receptor Pathway.
    Article Snippet: .. After cell isolation and extraction, singlecell sequencing libraries were constructed using the 10X Genomics platform [11] and sequencing was subsequently performed. ..

    Extraction:

    Article Title: Monocyte/macrophage-derived NLRP3 Promotes the Onset and Progression of Ankylosing Spondylitis Via the NOD-like Receptor Pathway.
    Article Snippet: .. After cell isolation and extraction, singlecell sequencing libraries were constructed using the 10X Genomics platform [11] and sequencing was subsequently performed. ..

    Single Cell:

    Article Title: Single-cell transcriptomics identifies regulatory T cell heterogeneity in gestational diabetes mellitus.
    Article Snippet: .. Single-cell mRNA, dual-indexed, sequencing libraries were generated following the Chromium Single Cell 5’ v2 protocol with Feature Barcode technology for Cell Surface Protein and Immune Receptor Mapping (CG000330, 10x Genomics). .. For each 10x run, patient samples were hash-tagged and pooled, using one antibody-derived tag per patient following the CITE-Seq protocol 55 (Biolegend) and then flowsorted to generate separate compartments of CD4+ T-cells and CD25+CD127lo T-cells before proceeding with the 10x assay.

    Article Title: Inducible deletion of DGAT1 and 2 from microglia exacerbates neurodegeneration and endolysosomal lipid accumulation in male PS19 mice.
    Article Snippet: Individual nuclei were then coupled to beads using the 10X Genomics Chromium controller. .. Single nuclei sequencing libraries were prepared following the 10X Genomics Chromium GEM-X Single Cell 3 ′ V4 kit protocol. .. Libraries were paired end sequenced on an Illumina NovaSeq X Plus platform (28 × 10 × 10X90X) to a minimum depth of 100,000 reads per nucleus.

    Generated:

    Article Title: Single-cell transcriptomics identifies regulatory T cell heterogeneity in gestational diabetes mellitus.
    Article Snippet: .. Single-cell mRNA, dual-indexed, sequencing libraries were generated following the Chromium Single Cell 5’ v2 protocol with Feature Barcode technology for Cell Surface Protein and Immune Receptor Mapping (CG000330, 10x Genomics). .. For each 10x run, patient samples were hash-tagged and pooled, using one antibody-derived tag per patient following the CITE-Seq protocol 55 (Biolegend) and then flowsorted to generate separate compartments of CD4+ T-cells and CD25+CD127lo T-cells before proceeding with the 10x assay.



    Similar Products

    cloud sequencing technology  (10X Genomics)
    86
    10X Genomics cloud sequencing technology
    Figure 3. The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation <t>sequencing-assembled</t> genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.
    Cloud Sequencing Technology, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cloud+sequencing+technology/libraries+sequencing/pm33748707-111-36-33
    Average 86 stars, based on 1 article reviews
    cloud sequencing technology - by Bioz Stars, 2026-09
    86/100 stars
    		  Buy from Supplier

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    Figure 3. The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation sequencing-assembled genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.

    Journal: iScience

    Article Title: Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content.

    doi: 10.1016/j.isci.2021.102219

    Figure Lengend Snippet: Figure 3. The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation sequencing-assembled genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.

    Article Snippet: Different from previous studies, in which stLFR was applied for animal or plant genomics, our method allowed the pooling and parallel sequencing of a large number of samples, which cannot be achieved by 10X Genomics read cloud sequencing technology (Goodwin et al., 2016).

    Techniques: Sequencing

    Figure 4. The longest chromosome sequence comparison (A and B) (A) Sequences alignment viewer and (B) accuracy evaluation of stLFR genomes, stLFR + ONT genomes, Canu- assembled genomes, and NGS genomes when compared with the complete genomes assembled by Unicycler using NGS and ONT reads for the strains E. coli K-12, Rufibacter sp. LB8, D. wulumuqiensis R12, and J. melonis M714.

    Journal: iScience

    Article Title: Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content.

    doi: 10.1016/j.isci.2021.102219

    Figure Lengend Snippet: Figure 4. The longest chromosome sequence comparison (A and B) (A) Sequences alignment viewer and (B) accuracy evaluation of stLFR genomes, stLFR + ONT genomes, Canu- assembled genomes, and NGS genomes when compared with the complete genomes assembled by Unicycler using NGS and ONT reads for the strains E. coli K-12, Rufibacter sp. LB8, D. wulumuqiensis R12, and J. melonis M714.

    Article Snippet: Different from previous studies, in which stLFR was applied for animal or plant genomics, our method allowed the pooling and parallel sequencing of a large number of samples, which cannot be achieved by 10X Genomics read cloud sequencing technology (Goodwin et al., 2016).

    Techniques: Sequencing, Comparison