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BioSpyder Technologies tempo-seq-r cloud-based software package
Tempo Seq R Cloud Based Software Package, supplied by BioSpyder Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cloud+sequencing+technology/tempo+seqr+data+analysis+platform/pm37074207-108-8-12
Average 90 stars, based on 1 article reviews
tempo-seq-r cloud-based software package - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Software:

Article Title: From vision toward best practices: Evaluating in vitro transcriptomic points of departure for application in risk assessment using a uniform workflow
Article Snippet: Other studies used the US EPA pilot pipeline ( https://github.com/USEPA/httrpl_pilot ) ( ; ), or other custom analysis pipelines (i.e., TempO-SeqR, v3.0, provided by BioSpyder for aligning TempO-Seq data, alongside custom data pre-processing steps in R) ( ; ; ).

Article Title: Radiogenomics Reveals Correlation between Quantitative Texture Radiomic Features of Biparametric MRI and Hypoxia-Related Gene Expression in Men with Localised Prostate Cancer.
Article Snippet: FASTQ files were aligned to the Human Whole Transcriptome v2.0 panel, which consists of 22,537 probes, using STAR in the TempO-SeqR software package [48] (BioSpyder Technologies, Carlsbad, CA, USA), with up to 2 mismatches allowed in the 50-nucleotide target sequence (https://www.bioclavis.co.uk/workflow, accessed on 9 June 2021) [49].

Article Title: House dust-derived mixtures of organophosphate esters alter the phenotype, function, transcriptome, and lipidome of KGN human ovarian granulosa cells.
Article Snippet: Organophosphate esters (OPEs), used as flame retardants and plasticizers, are present ubiquitously in the environment.. Previous studies suggest that exposure to OPEs is detrimental to female fertility in humans.. However, no experimental information is available on the effects of OPE mixtures on ovarian granulosa cells, which play essential roles in female reproduction.

Article Title: Substantiating chemical groups for read-across using molecular response profiles
Article Snippet: Changes in gene expression were assessed using a custom-194 designed, targeted TempO-SeqR assay, consisting of 2,378 probes of a BioSpyder platform 195 covering 1,988 D. magna genes mapped to human orthologs as described in Gruszczynska et 196 al. (2024).

Article Title: Per- and polyfluoroalkyl substances (PFAS) in mixtures show additive effects on transcriptomic points of departure in human liver spheroids
Article Snippet: FASTQ files were processed using the TempO-SeqR script v 3.0 provided by BioSpyder (BioSpyder Technologies, Inc), as implemented within our transcriptomics data processing pipeline ( https://github.com/R-ODAF/R-ODAF_Health_Canada ).

Article Title: Derivation of Transcriptomics-Based Points of Departure for 20 Per- or Polyfluoroalkyl Substances Using a Larval Fathead Minnow (Pimephales promelas) Reduced Transcriptome Assay.
Article Snippet: Traditional toxicity testing has been unable to keep pace with the introduction of new chemicals into commerce.. Consequently, there are limited or no toxicity data for many chemicals to which fish and wildlife may be exposed.. Per‐ and polyfluoroalkyl substances (PFAS) are emblematic of this issue in that ecological hazards of most PFAS remain uncharacterized.

Article Title: Application of a skin and liver Chip2 microphysiological model to investigate the route-dependent toxicokinetics and toxicodynamics of consumer-relevant doses of genistein.
Article Snippet: The results were submitted as raw counts and further analysed using the software package TempO-SeqR provided by Biospyder.

Article Title: APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1.
Article Snippet: RESEARCH ARTICLE | APRIL 19 2023 APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1 Carol E. Schrader; ... et. al J Immunol (2023) 210 (11): 1804–1814. https://doi.org/10.4049/jimmunol.2100946 Related Content Apurinic/Apyrimidinic Endonuclease 2 Is Necessary for Normal B Cell Development and Recovery of Lymphoid Progenitors after Chemotherapeutic Challenge J Immunol (February,2011) Apurinic/Apyrimidinic Endonuclease 2 Regulates the Expansion of Germinal Centers by Protecting against Activation- Induced Cytidine Deaminase–Independent DNA Damage in B Cells J Immunol (July,2014) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/210/11/1804/1645574/ji2100946.pdf by guest on 03 M ay 2025

Generated:

Article Title: From vision toward best practices: Evaluating in vitro transcriptomic points of departure for application in risk assessment using a uniform workflow
Article Snippet: Other studies used the US EPA pilot pipeline ( https://github.com/USEPA/httrpl_pilot ) ( ; ), or other custom analysis pipelines (i.e., TempO-SeqR, v3.0, provided by BioSpyder for aligning TempO-Seq data, alongside custom data pre-processing steps in R) ( ; ; ).

Article Title: Radiogenomics Reveals Correlation between Quantitative Texture Radiomic Features of Biparametric MRI and Hypoxia-Related Gene Expression in Men with Localised Prostate Cancer.
Article Snippet: FASTQ files were aligned to the Human Whole Transcriptome v2.0 panel, which consists of 22,537 probes, using STAR in the TempO-SeqR software package [48] (BioSpyder Technologies, Carlsbad, CA, USA), with up to 2 mismatches allowed in the 50-nucleotide target sequence (https://www.bioclavis.co.uk/workflow, accessed on 9 June 2021) [49].

Article Title: House dust-derived mixtures of organophosphate esters alter the phenotype, function, transcriptome, and lipidome of KGN human ovarian granulosa cells.
Article Snippet: Organophosphate esters (OPEs), used as flame retardants and plasticizers, are present ubiquitously in the environment.. Previous studies suggest that exposure to OPEs is detrimental to female fertility in humans.. However, no experimental information is available on the effects of OPE mixtures on ovarian granulosa cells, which play essential roles in female reproduction.

Article Title: Substantiating chemical groups for read-across using molecular response profiles
Article Snippet: Changes in gene expression were assessed using a custom-194 designed, targeted TempO-SeqR assay, consisting of 2,378 probes of a BioSpyder platform 195 covering 1,988 D. magna genes mapped to human orthologs as described in Gruszczynska et 196 al. (2024).

Article Title: Per- and polyfluoroalkyl substances (PFAS) in mixtures show additive effects on transcriptomic points of departure in human liver spheroids
Article Snippet: FASTQ files were processed using the TempO-SeqR script v 3.0 provided by BioSpyder (BioSpyder Technologies, Inc), as implemented within our transcriptomics data processing pipeline ( https://github.com/R-ODAF/R-ODAF_Health_Canada ).

Article Title: Derivation of Transcriptomics-Based Points of Departure for 20 Per- or Polyfluoroalkyl Substances Using a Larval Fathead Minnow (Pimephales promelas) Reduced Transcriptome Assay.
Article Snippet: Traditional toxicity testing has been unable to keep pace with the introduction of new chemicals into commerce.. Consequently, there are limited or no toxicity data for many chemicals to which fish and wildlife may be exposed.. Per‐ and polyfluoroalkyl substances (PFAS) are emblematic of this issue in that ecological hazards of most PFAS remain uncharacterized.

Article Title: Application of a skin and liver Chip2 microphysiological model to investigate the route-dependent toxicokinetics and toxicodynamics of consumer-relevant doses of genistein.
Article Snippet: The results were submitted as raw counts and further analysed using the software package TempO-SeqR provided by Biospyder.

Article Title: APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1.
Article Snippet: RESEARCH ARTICLE | APRIL 19 2023 APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1 Carol E. Schrader; ... et. al J Immunol (2023) 210 (11): 1804–1814. https://doi.org/10.4049/jimmunol.2100946 Related Content Apurinic/Apyrimidinic Endonuclease 2 Is Necessary for Normal B Cell Development and Recovery of Lymphoid Progenitors after Chemotherapeutic Challenge J Immunol (February,2011) Apurinic/Apyrimidinic Endonuclease 2 Regulates the Expansion of Germinal Centers by Protecting against Activation- Induced Cytidine Deaminase–Independent DNA Damage in B Cells J Immunol (July,2014) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/210/11/1804/1645574/ji2100946.pdf by guest on 03 M ay 2025

RNA Sequencing:

Article Title: From vision toward best practices: Evaluating in vitro transcriptomic points of departure for application in risk assessment using a uniform workflow
Article Snippet: Other studies used the US EPA pilot pipeline ( https://github.com/USEPA/httrpl_pilot ) ( ; ), or other custom analysis pipelines (i.e., TempO-SeqR, v3.0, provided by BioSpyder for aligning TempO-Seq data, alongside custom data pre-processing steps in R) ( ; ; ).

Article Title: Radiogenomics Reveals Correlation between Quantitative Texture Radiomic Features of Biparametric MRI and Hypoxia-Related Gene Expression in Men with Localised Prostate Cancer.
Article Snippet: FASTQ files were aligned to the Human Whole Transcriptome v2.0 panel, which consists of 22,537 probes, using STAR in the TempO-SeqR software package [48] (BioSpyder Technologies, Carlsbad, CA, USA), with up to 2 mismatches allowed in the 50-nucleotide target sequence (https://www.bioclavis.co.uk/workflow, accessed on 9 June 2021) [49].

Article Title: House dust-derived mixtures of organophosphate esters alter the phenotype, function, transcriptome, and lipidome of KGN human ovarian granulosa cells.
Article Snippet: Organophosphate esters (OPEs), used as flame retardants and plasticizers, are present ubiquitously in the environment.. Previous studies suggest that exposure to OPEs is detrimental to female fertility in humans.. However, no experimental information is available on the effects of OPE mixtures on ovarian granulosa cells, which play essential roles in female reproduction.

Article Title: Substantiating chemical groups for read-across using molecular response profiles
Article Snippet: Changes in gene expression were assessed using a custom-194 designed, targeted TempO-SeqR assay, consisting of 2,378 probes of a BioSpyder platform 195 covering 1,988 D. magna genes mapped to human orthologs as described in Gruszczynska et 196 al. (2024).

Article Title: Per- and polyfluoroalkyl substances (PFAS) in mixtures show additive effects on transcriptomic points of departure in human liver spheroids
Article Snippet: FASTQ files were processed using the TempO-SeqR script v 3.0 provided by BioSpyder (BioSpyder Technologies, Inc), as implemented within our transcriptomics data processing pipeline ( https://github.com/R-ODAF/R-ODAF_Health_Canada ).

Article Title: Derivation of Transcriptomics-Based Points of Departure for 20 Per- or Polyfluoroalkyl Substances Using a Larval Fathead Minnow (Pimephales promelas) Reduced Transcriptome Assay.
Article Snippet: Traditional toxicity testing has been unable to keep pace with the introduction of new chemicals into commerce.. Consequently, there are limited or no toxicity data for many chemicals to which fish and wildlife may be exposed.. Per‐ and polyfluoroalkyl substances (PFAS) are emblematic of this issue in that ecological hazards of most PFAS remain uncharacterized.

Article Title: Application of a skin and liver Chip2 microphysiological model to investigate the route-dependent toxicokinetics and toxicodynamics of consumer-relevant doses of genistein.
Article Snippet: The results were submitted as raw counts and further analysed using the software package TempO-SeqR provided by Biospyder.

Article Title: APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1.
Article Snippet: RESEARCH ARTICLE | APRIL 19 2023 APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1 Carol E. Schrader; ... et. al J Immunol (2023) 210 (11): 1804–1814. https://doi.org/10.4049/jimmunol.2100946 Related Content Apurinic/Apyrimidinic Endonuclease 2 Is Necessary for Normal B Cell Development and Recovery of Lymphoid Progenitors after Chemotherapeutic Challenge J Immunol (February,2011) Apurinic/Apyrimidinic Endonuclease 2 Regulates the Expansion of Germinal Centers by Protecting against Activation- Induced Cytidine Deaminase–Independent DNA Damage in B Cells J Immunol (July,2014) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/210/11/1804/1645574/ji2100946.pdf by guest on 03 M ay 2025

Sequencing:

Article Title: From vision toward best practices: Evaluating in vitro transcriptomic points of departure for application in risk assessment using a uniform workflow
Article Snippet: Other studies used the US EPA pilot pipeline ( https://github.com/USEPA/httrpl_pilot ) ( ; ), or other custom analysis pipelines (i.e., TempO-SeqR, v3.0, provided by BioSpyder for aligning TempO-Seq data, alongside custom data pre-processing steps in R) ( ; ; ).

Article Title: Radiogenomics Reveals Correlation between Quantitative Texture Radiomic Features of Biparametric MRI and Hypoxia-Related Gene Expression in Men with Localised Prostate Cancer.
Article Snippet: FASTQ files were aligned to the Human Whole Transcriptome v2.0 panel, which consists of 22,537 probes, using STAR in the TempO-SeqR software package [48] (BioSpyder Technologies, Carlsbad, CA, USA), with up to 2 mismatches allowed in the 50-nucleotide target sequence (https://www.bioclavis.co.uk/workflow, accessed on 9 June 2021) [49].

Article Title: House dust-derived mixtures of organophosphate esters alter the phenotype, function, transcriptome, and lipidome of KGN human ovarian granulosa cells.
Article Snippet: Organophosphate esters (OPEs), used as flame retardants and plasticizers, are present ubiquitously in the environment.. Previous studies suggest that exposure to OPEs is detrimental to female fertility in humans.. However, no experimental information is available on the effects of OPE mixtures on ovarian granulosa cells, which play essential roles in female reproduction.

Article Title: Substantiating chemical groups for read-across using molecular response profiles
Article Snippet: Changes in gene expression were assessed using a custom-194 designed, targeted TempO-SeqR assay, consisting of 2,378 probes of a BioSpyder platform 195 covering 1,988 D. magna genes mapped to human orthologs as described in Gruszczynska et 196 al. (2024).

Article Title: Per- and polyfluoroalkyl substances (PFAS) in mixtures show additive effects on transcriptomic points of departure in human liver spheroids
Article Snippet: FASTQ files were processed using the TempO-SeqR script v 3.0 provided by BioSpyder (BioSpyder Technologies, Inc), as implemented within our transcriptomics data processing pipeline ( https://github.com/R-ODAF/R-ODAF_Health_Canada ).

Article Title: Derivation of Transcriptomics-Based Points of Departure for 20 Per- or Polyfluoroalkyl Substances Using a Larval Fathead Minnow (Pimephales promelas) Reduced Transcriptome Assay.
Article Snippet: Traditional toxicity testing has been unable to keep pace with the introduction of new chemicals into commerce.. Consequently, there are limited or no toxicity data for many chemicals to which fish and wildlife may be exposed.. Per‐ and polyfluoroalkyl substances (PFAS) are emblematic of this issue in that ecological hazards of most PFAS remain uncharacterized.

Article Title: Application of a skin and liver Chip2 microphysiological model to investigate the route-dependent toxicokinetics and toxicodynamics of consumer-relevant doses of genistein.
Article Snippet: The results were submitted as raw counts and further analysed using the software package TempO-SeqR provided by Biospyder.

Article Title: APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1.
Article Snippet: RESEARCH ARTICLE | APRIL 19 2023 APE2 Promotes AID-Dependent Somatic Hypermutation in Primary B Cell Cultures That Is Suppressed by APE1 Carol E. Schrader; ... et. al J Immunol (2023) 210 (11): 1804–1814. https://doi.org/10.4049/jimmunol.2100946 Related Content Apurinic/Apyrimidinic Endonuclease 2 Is Necessary for Normal B Cell Development and Recovery of Lymphoid Progenitors after Chemotherapeutic Challenge J Immunol (February,2011) Apurinic/Apyrimidinic Endonuclease 2 Regulates the Expansion of Germinal Centers by Protecting against Activation- Induced Cytidine Deaminase–Independent DNA Damage in B Cells J Immunol (July,2014) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/210/11/1804/1645574/ji2100946.pdf by guest on 03 M ay 2025



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10X Genomics cloud sequencing technology
Figure 3. The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation <t>sequencing-assembled</t> genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.
Cloud Sequencing Technology, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cloud+sequencing+technology/libraries+sequencing/pm33748707-111-36-33
Average 86 stars, based on 1 article reviews
cloud sequencing technology - by Bioz Stars, 2026-10
86/100 stars
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Figure 3. The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation sequencing-assembled genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.

Journal: iScience

Article Title: Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content.

doi: 10.1016/j.isci.2021.102219

Figure Lengend Snippet: Figure 3. The longest chromosome sequence comparisons for strains (A–D) (A) E. coli K-12 using stLFR-assembled genome and the third-generation sequencing-assembled genome, (B) Rufibacter sp. LB8, (C) D. wulumuqiensis R12, and (D) J. melonis M714 using stLFR-assembled genomes, stLFR + ONT-assembled genomes, ONT + NGS Unicycler-assembled genomes, and ONT Canu-assembled genomes. The outermost circle is GC heatmap, the next circle is the histogram of GC (red: G > C; blue: G < (C), and the middle circle is gene density in chromosomes. The last two circles are the COG positive/negative annotation heatmaps, and the legend is shown at the bottom.

Article Snippet: Different from previous studies, in which stLFR was applied for animal or plant genomics, our method allowed the pooling and parallel sequencing of a large number of samples, which cannot be achieved by 10X Genomics read cloud sequencing technology (Goodwin et al., 2016).

Techniques: Sequencing

Figure 4. The longest chromosome sequence comparison (A and B) (A) Sequences alignment viewer and (B) accuracy evaluation of stLFR genomes, stLFR + ONT genomes, Canu- assembled genomes, and NGS genomes when compared with the complete genomes assembled by Unicycler using NGS and ONT reads for the strains E. coli K-12, Rufibacter sp. LB8, D. wulumuqiensis R12, and J. melonis M714.

Journal: iScience

Article Title: Comparison of different sequencing strategies for assembling chromosome-level genomes of extremophiles with variable GC content.

doi: 10.1016/j.isci.2021.102219

Figure Lengend Snippet: Figure 4. The longest chromosome sequence comparison (A and B) (A) Sequences alignment viewer and (B) accuracy evaluation of stLFR genomes, stLFR + ONT genomes, Canu- assembled genomes, and NGS genomes when compared with the complete genomes assembled by Unicycler using NGS and ONT reads for the strains E. coli K-12, Rufibacter sp. LB8, D. wulumuqiensis R12, and J. melonis M714.

Article Snippet: Different from previous studies, in which stLFR was applied for animal or plant genomics, our method allowed the pooling and parallel sequencing of a large number of samples, which cannot be achieved by 10X Genomics read cloud sequencing technology (Goodwin et al., 2016).

Techniques: Sequencing, Comparison