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clonepix2 colony picker  (Danaher Inc)


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    Structured Review

    Danaher Inc clonepix2 colony picker
    Clonepix2 Colony Picker, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clonepix2/ClonePix+2/us12435159-532-15-18
    Average 93 stars, based on 51 article reviews
    clonepix2 colony picker - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Suspension:

    Article Title: Endogenous Retrovirus-Like Particle-Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell-Surface Expression of Retroviral Envelope Protein.
    Article Snippet: suspended at a concentration of 2–5 × 105 input myeloma cells per mL in semi‐solid ClonaCell‐HY hybridoma selection medium D (StemCell Technologies, Vancouver, BC, Canada), supplemented with 5% H‐I FBS, 1 ng/mL mouse IL‐6, and 10 μg/mL fluorescein isothiocyanate (FITC)‐labeled F(ab′)2 goat anti‐mouse IgG (Jackson ImmunoResearch, Cedarlane, Burlington, ON) .. The cell suspension was plated in Nunc OmniTrays (Thermo Scientific) and incubated for 6 days at 37°C, 5% CO2. .. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐ secretor hybridoma clones into sterile 96‐well plates containing 200 μL of selection medium, with the HAT supplement replaced by the hypoxanthine‐thymidine (HT) supplement.

    Incubation:

    Article Title: Endogenous Retrovirus-Like Particle-Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell-Surface Expression of Retroviral Envelope Protein.
    Article Snippet: suspended at a concentration of 2–5 × 105 input myeloma cells per mL in semi‐solid ClonaCell‐HY hybridoma selection medium D (StemCell Technologies, Vancouver, BC, Canada), supplemented with 5% H‐I FBS, 1 ng/mL mouse IL‐6, and 10 μg/mL fluorescein isothiocyanate (FITC)‐labeled F(ab′)2 goat anti‐mouse IgG (Jackson ImmunoResearch, Cedarlane, Burlington, ON) .. The cell suspension was plated in Nunc OmniTrays (Thermo Scientific) and incubated for 6 days at 37°C, 5% CO2. .. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐ secretor hybridoma clones into sterile 96‐well plates containing 200 μL of selection medium, with the HAT supplement replaced by the hypoxanthine‐thymidine (HT) supplement.

    Clone Assay:

    Article Title: Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope Protein
    Article Snippet: The cell suspension was plated in Nunc OmniTrays (Thermo Scientific) and incubated for 6 days at 37°C, 5% CO2. .. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐secretor hybridoma clones into sterile 96‐well plates containing 200 μL of selection medium, with the HAT supplement replaced by the hypoxanthine‐thymidine (HT) supplement. ..

    Sterility:

    Article Title: Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope Protein
    Article Snippet: The cell suspension was plated in Nunc OmniTrays (Thermo Scientific) and incubated for 6 days at 37°C, 5% CO2. .. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐secretor hybridoma clones into sterile 96‐well plates containing 200 μL of selection medium, with the HAT supplement replaced by the hypoxanthine‐thymidine (HT) supplement. ..

    Selection:

    Article Title: Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope Protein
    Article Snippet: The cell suspension was plated in Nunc OmniTrays (Thermo Scientific) and incubated for 6 days at 37°C, 5% CO2. .. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐secretor hybridoma clones into sterile 96‐well plates containing 200 μL of selection medium, with the HAT supplement replaced by the hypoxanthine‐thymidine (HT) supplement. ..

    HAT Assay:

    Article Title: Endogenous Retrovirus‐Like Particle‐Deficient CHO Cells Can be Generated by CRISPR or shRNA and Enriched Based on Cell‐Surface Expression of Retroviral Envelope Protein
    Article Snippet: The cell suspension was plated in Nunc OmniTrays (Thermo Scientific) and incubated for 6 days at 37°C, 5% CO2. .. A robotized mammalian cell clone picker (Clonepix2; Molecular Devices, Boston, MA) was used to pick and transfer fluorescent antibody‐secretor hybridoma clones into sterile 96‐well plates containing 200 μL of selection medium, with the HAT supplement replaced by the hypoxanthine‐thymidine (HT) supplement. ..

    Colony Assay:

    Article Title: Anti-human GRP94 antibodies and uses
    Article Snippet: .. Growth plates were imaged by bright light, FITC, and AF647 fluorescent detection channels in the ClonePix2 colony picker (Molecular Devices, UK) and hybridoma colonies were picked into 200 microliters of ClonaCell-HY recovery medium (Stem Cell Technologies, UK). .. Grp94 ELISA 50 microliter/well of 2 microgram/ml Grp94 protein in PBS buffer pH=7.4 was used to coat a MICROLON medium binding ELISA plate (Greiner Bio One Ltd) and incubated at 4° C. overnight.



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