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Structured Review

Click Chemistry Tools c10269 click it cell reaction buffer kit
C10269 Click It Cell Reaction Buffer Kit, supplied by Click Chemistry Tools, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/click+reaction+buffer/buffer+chemistry+kit+reaction/pm41219002-167-30-36
Average 86 stars, based on 1 article reviews
c10269 click it cell reaction buffer kit - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

other:

Article Title: Functional Super-Resolution Microscopy and Its Applications in Biophysics and Cell Biology
Article Snippet: Cells on the surface of the plate were then lysed in the Triton lysis buffer (1% TritonX-100 [Sigma-Aldrich T8787], 137 mM NaCl [Sigma-Aldrich S9888], 50 mM Tris-HCl pH 7.5, 1x protease inhibitor cocktail [Thermo Scientific 87786], and 1x phosphatase inhibitor cocktail [Sigma-Aldrich P0044] in water) for 30 min on ice.

Labeling:

Article Title: Potent Inhibition of Necroptosis by Simultaneously Targeting Multiple Effectors of the Pathway.
Article Snippet: .. Click labeling was then performed with TAMRA-azide (Click Chemistry Tools) using the Click Chemistry Protein Reaction Buffer Kit (Click Chemistry Tools) according to the manufacturer’s protocol, but scaled down by half. ..

Blocking Assay:

Article Title: Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neurons.
Article Snippet: .. After blocking, neurons were incubated for 3 h with the FUNCAT reaction, following the kit’s instructions to tag AHA-incorporated proteins with an alkynefluorophore Alexa Fluor 555 through the click reaction (C10269; CLICK-iT Cell Reaction Buffer Kit, Click Chemistry Tools). ..

Incubation:

Article Title: Group 1 mGluR stimulation rescues APOE4-mediated translation defects in neurons.
Article Snippet: .. After blocking, neurons were incubated for 3 h with the FUNCAT reaction, following the kit’s instructions to tag AHA-incorporated proteins with an alkynefluorophore Alexa Fluor 555 through the click reaction (C10269; CLICK-iT Cell Reaction Buffer Kit, Click Chemistry Tools). ..

Article Title: Exploring single-cell metabolism and its control on cell growth signals using fluorescent biosensors.
Article Snippet: To estimate global nascent protein synthesis rate, we pulse-labeled cells with the puromycin analog O-propargyl-puromycin (OPP; Click Chemistry Tools #1407) at 10 μM final concentration for 30 minutes before the end of live-cell imaging. .. After fixation, permeabilization, and fluorophore bleaching as described earlier, cells were then incubated with click chemistry reaction buffer (10 μM Azide dye + 4mM CuSO4 + 50 mM Ascorbic acid in 100 mM Tris Buffer pH 8.5) containing Alexa 647 Azide dye (Click Chemistry Tools #1299) for 1 hour. ..



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