cli095 (MedChemExpress)

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MedChemExpress cli095

Figure 2. Lipotoxicity mediated by TLR4 is closely linked to TMEM24 (A) β-Cells were treated with a TLR4 inhibitor <t>(CLI095)</t> or agonist (LPS), and TLR4 protein levels were detected by western blot analysis. (B) β-Cells were treated with CLI095 or LPS, and TMEM24 protein levels were detected by western blot analysis. (C) Insulin secretion from β-cells treated with CLI095 or LPS. (D) Apoptosis rates were determined by flow cytometry across groups. (E) β-Cells were transfected with siRNA for 24 h. TLR4 knockdown efficiency was then determined by western blot analysis. (F) TMEM24 protein expression across groups was detected by western blot analysis. (G) Insulin secretion across groups. (H) Apoptosis rates were determined by flow cytometry across groups. *P < 0.05 vs the NC group and #P < 0.05 vs the PA group. Data are presented as the mean ± SD (n = 3).

Cli095, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cli095/Resatorvid/pm40170616-65-14-15
Average 98 stars, based on 666 article reviews

cli095 - by Bioz Stars, 2026-10

98/100 stars

Images

1) Product Images from "TLR4 mediates lipotoxic β-cell dysfunction by inhibiting the TMEM24/PI3K/AKT pathway."

Article Title: TLR4 mediates lipotoxic β-cell dysfunction by inhibiting the TMEM24/PI3K/AKT pathway.

Journal: Acta biochimica et biophysica Sinica

doi: 10.3724/abbs.2025045

Figure 2. Lipotoxicity mediated by TLR4 is closely linked to TMEM24 (A) β-Cells were treated with a TLR4 inhibitor (CLI095) or agonist (LPS), and TLR4 protein levels were detected by western blot analysis. (B) β-Cells were treated with CLI095 or LPS, and TMEM24 protein levels were detected by western blot analysis. (C) Insulin secretion from β-cells treated with CLI095 or LPS. (D) Apoptosis rates were determined by flow cytometry across groups. (E) β-Cells were transfected with siRNA for 24 h. TLR4 knockdown efficiency was then determined by western blot analysis. (F) TMEM24 protein expression across groups was detected by western blot analysis. (G) Insulin secretion across groups. (H) Apoptosis rates were determined by flow cytometry across groups. *P < 0.05 vs the NC group and #P < 0.05 vs the PA group. Data are presented as the mean ± SD (n = 3).

Figure Legend Snippet: Figure 2. Lipotoxicity mediated by TLR4 is closely linked to TMEM24 (A) β-Cells were treated with a TLR4 inhibitor (CLI095) or agonist (LPS), and TLR4 protein levels were detected by western blot analysis. (B) β-Cells were treated with CLI095 or LPS, and TMEM24 protein levels were detected by western blot analysis. (C) Insulin secretion from β-cells treated with CLI095 or LPS. (D) Apoptosis rates were determined by flow cytometry across groups. (E) β-Cells were transfected with siRNA for 24 h. TLR4 knockdown efficiency was then determined by western blot analysis. (F) TMEM24 protein expression across groups was detected by western blot analysis. (G) Insulin secretion across groups. (H) Apoptosis rates were determined by flow cytometry across groups. *P < 0.05 vs the NC group and #P < 0.05 vs the PA group. Data are presented as the mean ± SD (n = 3).

Techniques Used: Western Blot, Flow Cytometry, Transfection, Knockdown, Expressing

Control:

Article Title: HMGB1-mediated endoplasmic reticulum stress in monocyte-derived dendritic cells regulates CD4+ T cell immune responses in myasthenia gravis.
Article Snippet: 4μ8C (Catalog No. HY19707, MedChemExpress, Princeton, USA) was utilized to inhibit IRE1 endoribonuclease (RNase) activity. .. On day 6, moDCs were pretreated with the TLR4 inhibitor TAK-242 and/or the RAGE inhibitor FPS-ZM1 (Catalog No. HY-11109/HY-19370, MedChemExpress, Princeton, NJ, USA) for 1 h. MoDCs were then stimulated with PBS (Control), HMGB1 (1 μg/mL), 4μ8C (20 μM), or HMGB1 plus 4μ8C in fresh culture medium for 48 h. Splenic monocytes and mononuclear cells (SMCs) from mice were isolated via gradient centrifugation using the extraction kit (Catalog No. TBD2011MPK, TBD Science, Tianjin, China). .. Red blood cells were removed by red blood cell lysis buffer (Cat. No. G2015-500ML, Servicebio, Wuhan, China).

Isolation:

Gradient Centrifugation:

Extraction:

Concentration Assay:

Article Title: Integrated Single‐Cell and TCR Profiling Reveals Protection‐Associated CD8 + T Cell Subsets Linked to Viral Control in PRRSV
Article Snippet: IFN‐γ‐secreting cells were detected using a biotinylated anti‐pig IFN‐γ detection antibody and visualized using an ImmunoSpot image analyzer. .. For the TLR‐4 or TLR‐8 loss‐function assay, the CD14‐deleted cells were pre‐incubated with TAK‐242 (MCE, #243984‐11‐4) or CU‐CPT8m (MCE, #HY‐112050) at a final concentration of 5 μM for 2 h, followed by the addition of virus, and ELISpot was then performed. ..

Virus:

Enzyme-linked Immunospot:

other:

Article Title: Allicin alleviates myocardial PANoptosis during ischemia-reperfusion by inhibiting TLR4 activation.
Article Snippet: Background: PANoptosis is a newly identified form of programmed cell death characterized by necroptosis, pyroptosis, and apoptosis.. However, the mechanism of myocardial PANoptosis in myocardial ischemiareperfusion (MI/R) remains unclear.. Allicin is a promising drug for MI/R treatment, and the targets for myocardial PANoptosis remain to be explored.

Biomarker Discovery:

Article Title: Liqi Huoxue dripping pills alleviate myocardial ischemia-reperfusion injury with improved mitochondrial biogenesis and reduced ferroptosis: Involvement of S100A8/A9-TLR4-ERK signaling.
Article Snippet: Recombinant rat S100A8 protein (HY-P71275) and S100A9 protein (PDER100157) were purchased from MedChemExpress (Monmouth Junction, NJ, USA) and Elabscience (Wuhan, China), respectively, and used for rescue experiments (1000 ng/ml each). .. U0126 (10 μM; Thermo Scientific Chemicals, Waltham, MA, USA; J61246.MB), ferrostatin-1 (Fer-1; 2 μM; Macklin, Shanghai, China; CAS No 347174-05–4), and TAK-242 (3 mg⋅kg–1⋅d–1, i.p.; MedChemExpress, Shanghai, China; HY-11109) were used for pharmacological pathway validation experiments. .. Primary antibodies used in this study included: anti-S100A8/A9 (ab288715, 1:2000), anti-PGC-1α (ab191838, 1:2000), anti-GPX4 (ab125066, 1:2000), anti-SLC7A11 (ab307601, 1:2000), anti-ACSL4 (ab155282, 1:2000), and anti-Cyt-c (ab133504, 1:1000), anti-p-p65 (ab184032, 1:1000) from Abcam (Cambridge, UK); anti-TLR4 (19,811- 1-AP, 1:2000), anti-NDUFA9 (20,312-1-AP, 1:2000), anti-FTH1 (11,682-1-AP, 1:2000), and anti-GAPDH (10,494-1-AP, 1:5000) from Proteintech (Wuhan, China); anti-TFR1 (bs-41400R, 1:1000) from BIOSS (Beijing, China); anti-p65 (AF2006, 1:5000) from Affinity Biosciences (Changzhou, China); anti-p-TLR4 (PA5–105,713, 1:1000) from Thermo Fisher Scientific (Waltham, MA, USA); anti-ERK1/2 (YM8336, 1:4000), anti-p-ERK1/2 (YM8452, 1:2000), and anti-NRF1 (YM8513, 1:2000) from Immunoway (Plano, TX, USA).

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