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november 2017 methods elisa plates  (Greiner Bio)


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    Structured Review

    Greiner Bio november 2017 methods elisa plates
    November 2017 Methods Elisa Plates, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 97/100, based on 875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/clearing+method/Microplate+96+Well+Ps+F-Bottom+Clear/us11680942-308-56-61
    Average 97 stars, based on 875 article reviews
    november 2017 methods elisa plates - by Bioz Stars, 2026-11
    97/100 stars

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    96 Well Plates:

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    Article Title: Cross-reactive anti-prophage antibodies and bacterial heteroresistance implicated in phage therapeutic failure.
    Article Snippet: Phage therapy is an exciting strategy against antimicrobial-resistant bacterial infections, but critical knowledge gaps regarding its clinical application persist.. Here we present a case study of a 22-year-old male patient with cystic fibrosis, presenting with a recurrent, invasive and ultimately lethal Bordetella bronchialis infection, who failed compassionate-use phage therapy.. Using longitudinal clinical samples, we found that our patient harbored pre-existing antibodies against active prophages induced from the genome of the infecting pathogen.

    Microscopy:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Incubator:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Control:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Polymerase Chain Reaction:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Adhesive:

    Article Title: Efficient genome editing using CRISPR-Cas9 in reef-building corals.
    Article Snippet: Coral reefs are one of the most biodiverse and productive ecosystems on Earth.. However, corals are currently under threat from increasing ocean temperatures driven by climate change.. Despite the known importance of these fragile ecosystems, our understanding of the molecular mechanisms driving ecologically important traits has been constrained by a lack of genetic tools for functional characterization.

    Incubation:

    Article Title: Turning RuBisCO-enriched snap pea protein concentrates into function: A novel plant protein for fat-free whipped cream alternatives
    Article Snippet: With the growing concerns over the environmental and ethical impacts associated with the use of animal-based protein, there is a need to explore new sustainable alternative protein sources.. In this study, the potential of a novel snap pea protein (SPP) as a functional ingredient for aerated food products development was evaluated.. Through the in-house established and validated miniaturized assay, the techno-functional properties, including emulsifying, foaming, and gelling properties of SPP were evaluated and compared with egg white protein (EWP) and mung bean protein (MBP).

    Saline:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Staining:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Transfection:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Modification:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Cell Culture:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Cell Counting:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Automated:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Sterility:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..

    Qubit:

    Article Title: Protocol for Designing and Interpreting Minigene Assays to Validate Candidate Splice‐Altering Variants Using Long‐read Sequencing
    Article Snippet: .. Complete growth medium (see recipe) Mammalian cell line to transfect (e.g., HEK‐293, ATCC #CRL‐1573) Phosphate‐buffered saline (PBS), pH 7.4 (Gibco, cat. no. 10010023) Trypsin‐EDTA (0.25%), phenol red (Thermo Fisher, cat. no. 25200072) Trypan Blue Stain (Invitrogen, cat no. T10282 ) Lipofectamine 3000 transfection reagent (Invitrogen, cat. no. L3000008) Opti‐MEM reduced serum medium (Gibco, cat. no. 31985062) Minigene plasmids as designed in Basic Protocol Dulbecco's Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) Cycloheximide (Sigma‐Aldrich, cat. no. C4859) RNeasy Mini Kit (Qiagen, cat. no. 74106) SuperScript III First‐Strand Synthesis SuperMix (Invitrogen, cat. no. 18080400) Labculture Class II Type A2 Biological Safety Cabinet (Esco Lifesciences) Julabo Eco Temp TW12 water bath (Sigma‐Aldrich) Cell Culture Flask, 250 mL, 75 cm2, Ps, Red Filter Screw Cap, Clear (Greiner Bio‐One, cat. no. 658175) Tube, 15 mL, Pp, 17/120 Mm, Conical Bottom (Greiner Bio‐One, cat. no. 188271) Heracel VIOS 160i CO 2 Incubator (Thermo Fisher Scientific) Cell Culture Multiwell Plate, 6 Well, Ps, Clear (Greiner Bio‐One, cat. no. 657160) Countess Cell Counting Chamber Slides (Invitrogen, cat. no. C10228 ) Automated Cell Counter ‐ Countess II FL (Life Technologies) Microplate, 96 Well, Ps, F‐Bottom, Clear (Greiner Bio‐One, cat. no. 655101) Mastercycler Nexus Gradient PCR thermocycler (Eppendorf) Centrifuge 5810 R (Eppendorf) Tube 1.5 mL Sterile (SSI Bio, cat. no. 1210‐S0) Reax top test tube shaker (vortex) (Heidolph) MIKRO 220 Microcentrifuge (Hettich) Qubit 4 Fluorometer (Invitrogen) ..



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    Image Search Results


    Journal: STAR Protocols

    Article Title: Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

    doi: 10.1016/j.xpro.2022.101703

    Figure Lengend Snippet:

    Article Snippet: The described protocol is an adaptation of the whole-mount immunostaining protocol developed by ( ) and the RapiClear 1.47 clearing method developed by the SunJin Lab ( ; ).

    Techniques: Recombinant, Fluorsave, Software, Microscopy