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Sunjin Co Ltd rapiclear 1.47 clearing method

Rapiclear 1.47 Clearing Method, supplied by Sunjin Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clearing+method/rapiclear+1+52/pmc09508478-326-17-24
Average 90 stars, based on 1 article reviews
rapiclear 1.47 clearing method - by Bioz Stars, 2026-09
90/100 stars

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1) Product Images from "Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing"

Article Title: Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

Journal: STAR Protocols

doi: 10.1016/j.xpro.2022.101703


Figure Legend Snippet:

Techniques Used: Recombinant, Fluorsave, Software, Microscopy

Related Articles

Incubation:

Article Title: Molecular analyses of individual variation in honey bee sociability
Article Snippet: The samples were washed in PBS and cleared with RapiClear® 1.49 (SunJin Lab Co, Hsinchu City, Taiwan) using an established protocol (Bekkouche et al., 2020).

Article Title: High iASPP (PPP1R13L) expression is an independent predictor of adverse clinical outcome in acute myeloid leukemia (AML).
Article Snippet: Last, immunostained thick femoral slices were incubated in RapiClear 1.52 (SunJin Lab, Taiwan), for a minimum of 6 hours, to increase imaging depth without loss of signal intensity.

Article Title: Fate mapping in mouse demonstrates early secretory differentiation directly from Lgr5+ intestinal stem cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti Human Lysozyme EC 3.2.1.17 (Concentrate) Agilent Dako Cat #: A0099; RRID: AB_2341230 Mouse Monoclonal anti BrdU (MoBU-1) Santa Cruz Cat #: sc-51514; RRID: AB_626519 GFP polyclonal antibody ThermoFisher Cat #: a11122; RRID: AB_221569 Alexa Fluor 647 Donkey anti Rabbit IgG (H+L) ThermoFisher Scientific Cat #: A-31573; RRID: AB_2536183 Alexa Fluor 555 Goat anti Mouse IgG2a (H+L) ThermoFisher Scientific Cat #: A-21137; RRID: AB_2535776 Anti Mouse EpCAM APC (G8.8) eBioscience Cat #: 17-5791-80, RRID: AB_1659714 PE-Cy7 Rat Anti Mouse CD31 BD biosciences Cat #: 561410; RRID: AB_10612003 PE-Cy7 Rat Anti Mouse CD45 BD biosciences Cat #: 552848; RRID: AB_394489 TotalSeq-A0301 anti-mouse Hashtag 1 Antibody Biolegend Cat #: 155801; RRID: AB_2750032 TotalSeq-A0302 anti-mouse Hashtag 2 Antibody Biolegend Cat #: 155803; RRID: AB_2750033 TotalSeq-A0303 anti-mouse Hashtag 3 Antibody Biolegend Cat #: 155805; RRID: AB_2750034 TotalSeq-A0304 anti-mouse Hashtag 4 Antibody Biolegend Cat #: 155807; RRID: AB_2750035 TotalSeq-A0305 anti-mouse Hashtag 5 Antibody Biolegend Cat #: 155809; RRID: AB_2750036 TotalSeq-A0306 anti-mouse Hashtag 6 Antibody Biolegend Cat #: 155811; RRID: AB_2750037 TotalSeq-A0307 anti-mouse Hashtag 7 Antibody Biolegend Cat #: 155813; RRID: AB_2750039 TotalSeq-A0308 anti-mouse Hashtag 8 Antibody Biolegend Cat #: 155815; RRID: AB_2750040 Chemicals, peptides, and recombinant proteins 4-hydroxytamoxifen Sigma-Aldrich Cat #: H6278 EdU (5-ethynyl-20-deoxyuridine) Invitrogen / Thermo Fisher Scientific Cat #: A10044 BrdU (5-Bromo-20-Deoxyuridine) Invitrogen Cat #: 11594167 N,N,N0,N0-Tetrakis(2-hydroxypropyl)ethylenediamine Sigma-Aldrich Cat #: 122262 Urea Sigma-Aldrich Cat #: U5378 Triton x-100 Sigma-Aldrich Cat #: X100 Bovine Serum Albumin Sigma-Aldrich Cat #: A9576 RapiClear 1.52 Sunjin Lab Cat #: RC152002 Normal Goat Serum Sigma-Aldrich Cat #: G9023 HBSS Ca2+Mg2+ free ThermoFischer Scientific Cat #: 14175095 UltraPure 0.5M EDTA Invitrogen Cat #: 15575-020 HEPES buffer Gibco/Life Technologies Cat #: 15630-056 TrypLE Express ThermoFisher Scientific Cat #: 12605036 Y-27632 dihydrochloride Sigma-Aldrich Cat #: Y0503 N-Acetyl-L-cysteine Sigma-Aldrich Cat #: A9165 DNaseI Roche Cat #: 10104159001 aDMEM/F12 Gibco/Life Technologies Cat #: 12634-028 0.1%BSA PBS Sigma-Aldrich Cat #: A4503-100 DAPI Sigma-Aldrich Cat #: D9542 (Continued on next page) e1 Developmental Cell 60, 1–9.e1–e6, May 5, 2025

Article Title: Very Low‐Intensity Ultrasound Facilitates Glymphatic Influx and Clearance via Modulation of the TRPV4‐AQP4 Pathway
Article Snippet: After washing three times with PBS, the tissues were cleared and incubated with RapiClear® 1.49 (SunJin Lab Co., Hsinchu City, Taiwan) for 1 h to promote transparency and were then mounted with fresh RapiClear®.

Article Title: PIEZO2-dependent rapid pain system in humans.
Article Snippet: The free-floating skin sections were stained and cleared with RapiClear 1.47 (Sunjin Lab, Hsinchu City, Taiwan), according to the manufacturer’s protocol.

Article Title: The Formation of Human Arteriovenous Malformation Organoids and Their Characteristics
Article Snippet: The samples were then dehydrated in increasing concentrations of methanol (50%, 80%, and 100%) and incubated with RapiClear ® 1.47 (RC147001, SUNJin Lab, Hsinchu City, Taiwan) for clearing at a temperature of 25 °C overnight (>16 h) in the dark.

Article Title: The Formation of Human Arteriovenous Malformation Organoids and Their Characteristics.
Article Snippet: The samples were then dehydrated in increasing concentrations of methanol (50%, 80%, and 100%) and incubated with RapiClear® 1.47 (RC147001, SUNJin Lab, Hsinchu City, Taiwan) for clearing at a temperature of 25 ◦C overnight (>16 h) in the dark.

Article Title: Axonal Selectivity of Myelination by Single Oligodendrocytes Established During Development in Mouse Cerebellar White Matter.
Article Snippet: The slices were washed three times in PBS for 10 min each at room temperature (RT), and immunostaining was performed per the manufacturer's protocol of RapiClear 1.52 (RC152001, Sunjin Laboratory, Taiwan, China) with minor modifications.

Imaging:

Article Title: Molecular analyses of individual variation in honey bee sociability
Article Snippet: The samples were washed in PBS and cleared with RapiClear® 1.49 (SunJin Lab Co, Hsinchu City, Taiwan) using an established protocol (Bekkouche et al., 2020).

Article Title: High iASPP (PPP1R13L) expression is an independent predictor of adverse clinical outcome in acute myeloid leukemia (AML).
Article Snippet: Last, immunostained thick femoral slices were incubated in RapiClear 1.52 (SunJin Lab, Taiwan), for a minimum of 6 hours, to increase imaging depth without loss of signal intensity.

Article Title: Fate mapping in mouse demonstrates early secretory differentiation directly from Lgr5+ intestinal stem cells.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit Polyclonal anti Human Lysozyme EC 3.2.1.17 (Concentrate) Agilent Dako Cat #: A0099; RRID: AB_2341230 Mouse Monoclonal anti BrdU (MoBU-1) Santa Cruz Cat #: sc-51514; RRID: AB_626519 GFP polyclonal antibody ThermoFisher Cat #: a11122; RRID: AB_221569 Alexa Fluor 647 Donkey anti Rabbit IgG (H+L) ThermoFisher Scientific Cat #: A-31573; RRID: AB_2536183 Alexa Fluor 555 Goat anti Mouse IgG2a (H+L) ThermoFisher Scientific Cat #: A-21137; RRID: AB_2535776 Anti Mouse EpCAM APC (G8.8) eBioscience Cat #: 17-5791-80, RRID: AB_1659714 PE-Cy7 Rat Anti Mouse CD31 BD biosciences Cat #: 561410; RRID: AB_10612003 PE-Cy7 Rat Anti Mouse CD45 BD biosciences Cat #: 552848; RRID: AB_394489 TotalSeq-A0301 anti-mouse Hashtag 1 Antibody Biolegend Cat #: 155801; RRID: AB_2750032 TotalSeq-A0302 anti-mouse Hashtag 2 Antibody Biolegend Cat #: 155803; RRID: AB_2750033 TotalSeq-A0303 anti-mouse Hashtag 3 Antibody Biolegend Cat #: 155805; RRID: AB_2750034 TotalSeq-A0304 anti-mouse Hashtag 4 Antibody Biolegend Cat #: 155807; RRID: AB_2750035 TotalSeq-A0305 anti-mouse Hashtag 5 Antibody Biolegend Cat #: 155809; RRID: AB_2750036 TotalSeq-A0306 anti-mouse Hashtag 6 Antibody Biolegend Cat #: 155811; RRID: AB_2750037 TotalSeq-A0307 anti-mouse Hashtag 7 Antibody Biolegend Cat #: 155813; RRID: AB_2750039 TotalSeq-A0308 anti-mouse Hashtag 8 Antibody Biolegend Cat #: 155815; RRID: AB_2750040 Chemicals, peptides, and recombinant proteins 4-hydroxytamoxifen Sigma-Aldrich Cat #: H6278 EdU (5-ethynyl-20-deoxyuridine) Invitrogen / Thermo Fisher Scientific Cat #: A10044 BrdU (5-Bromo-20-Deoxyuridine) Invitrogen Cat #: 11594167 N,N,N0,N0-Tetrakis(2-hydroxypropyl)ethylenediamine Sigma-Aldrich Cat #: 122262 Urea Sigma-Aldrich Cat #: U5378 Triton x-100 Sigma-Aldrich Cat #: X100 Bovine Serum Albumin Sigma-Aldrich Cat #: A9576 RapiClear 1.52 Sunjin Lab Cat #: RC152002 Normal Goat Serum Sigma-Aldrich Cat #: G9023 HBSS Ca2+Mg2+ free ThermoFischer Scientific Cat #: 14175095 UltraPure 0.5M EDTA Invitrogen Cat #: 15575-020 HEPES buffer Gibco/Life Technologies Cat #: 15630-056 TrypLE Express ThermoFisher Scientific Cat #: 12605036 Y-27632 dihydrochloride Sigma-Aldrich Cat #: Y0503 N-Acetyl-L-cysteine Sigma-Aldrich Cat #: A9165 DNaseI Roche Cat #: 10104159001 aDMEM/F12 Gibco/Life Technologies Cat #: 12634-028 0.1%BSA PBS Sigma-Aldrich Cat #: A4503-100 DAPI Sigma-Aldrich Cat #: D9542 (Continued on next page) e1 Developmental Cell 60, 1–9.e1–e6, May 5, 2025

Article Title: Very Low‐Intensity Ultrasound Facilitates Glymphatic Influx and Clearance via Modulation of the TRPV4‐AQP4 Pathway
Article Snippet: After washing three times with PBS, the tissues were cleared and incubated with RapiClear® 1.49 (SunJin Lab Co., Hsinchu City, Taiwan) for 1 h to promote transparency and were then mounted with fresh RapiClear®.

Article Title: PIEZO2-dependent rapid pain system in humans.
Article Snippet: The free-floating skin sections were stained and cleared with RapiClear 1.47 (Sunjin Lab, Hsinchu City, Taiwan), according to the manufacturer’s protocol.

Article Title: The Formation of Human Arteriovenous Malformation Organoids and Their Characteristics
Article Snippet: The samples were then dehydrated in increasing concentrations of methanol (50%, 80%, and 100%) and incubated with RapiClear ® 1.47 (RC147001, SUNJin Lab, Hsinchu City, Taiwan) for clearing at a temperature of 25 °C overnight (>16 h) in the dark.

Article Title: The Formation of Human Arteriovenous Malformation Organoids and Their Characteristics.
Article Snippet: The samples were then dehydrated in increasing concentrations of methanol (50%, 80%, and 100%) and incubated with RapiClear® 1.47 (RC147001, SUNJin Lab, Hsinchu City, Taiwan) for clearing at a temperature of 25 ◦C overnight (>16 h) in the dark.

Article Title: Axonal Selectivity of Myelination by Single Oligodendrocytes Established During Development in Mouse Cerebellar White Matter.
Article Snippet: The slices were washed three times in PBS for 10 min each at room temperature (RT), and immunostaining was performed per the manufacturer's protocol of RapiClear 1.52 (RC152001, Sunjin Laboratory, Taiwan, China) with minor modifications.



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Image Search Results


Journal: STAR Protocols

Article Title: Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

doi: 10.1016/j.xpro.2022.101703

Figure Lengend Snippet:

Article Snippet: The described protocol is an adaptation of the whole-mount immunostaining protocol developed by ( ) and the RapiClear 1.47 clearing method developed by the SunJin Lab ( ; ).

Techniques: Recombinant, Fluorsave, Software, Microscopy