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Tokyo Chemical Industry cubic tissue clearing method
Cubic Tissue Clearing Method, supplied by Tokyo Chemical Industry, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/clearing+method/tissue+clearing+reagent+cubic+l/pm36435035-101-4-15
Average 90 stars, based on 1 article reviews
cubic tissue clearing method - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Staining:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.

Refractive Index:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.

Fluorescence:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.

Incubation:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.

Blocking Assay:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.

Recombinant:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.

Software:


Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The samples were immersed in the tissue-clearing reagent CUBIC-L (Tokyo Chemical Industry, Tokyo, Japan) overnight, permeabilized with 1% Triton X-100 in PBS for 2 h, and then incubated in a blocking solution for 2 h (1% bovine serum albumin, 3% normal goat serum, and 0.2% Triton X-100 in PBS).

Article Title: Lysosomal “TRAP”: a neotype modality for clearance of viruses and variants
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidin-FITC.

Article Title: Lysosomal "TRAP": a neotype modality for clearance of viruses and variants.
Article Snippet: The collected lungs were processed with Tissue-Clearing Reagent CUBIC (TCI) for tissue-clearing according to the manufacturer’s instructions, which followed the fluorescence staining of lung trachea by streptavidinFITC.

Article Title: A triple growth factor strategy for optimizing bone augmentation in mice.
Article Snippet: Funding information Japan Society for the Promotion of Science, Grant/Award Numbers: 20K10007, 22KK0138 Abstract With dental implant treatment becoming the gold standard, the need for effective bone augmentation prior to implantation has grown.. This study aims to evaluate a bone augmentation strategy integrating three key growth factors: bone morphogenetic protein-2 (BMP-2), insulin-like growth factor 1 (IGF-1), and vascular endothelial growth factor (VEGF).. Collagen scaffolds incorporating BMP-2, IGF-1, or VEGF were fabricated and categorized into five groups based on their content: scaffold alone; BMP-2 alone (BMP-2); BMP-2 and IGF-1 (BI); BMP-2, IGF-1, and VEGF (BIV); and BMP-2 and IGF-1 with an earlier release of VEGF (BI + V).

Article Title: Age-Dependent Differences in Radiation-Induced DNA Damage Responses in Intestinal Stem Cells
Article Snippet: The specimens were transferred to 96-well plates and immersed in tissue-clearing reagent CUBIC-R (Tokyo Chemical Industry, Tokyo, Japan) for image acquisition.

Article Title: Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo
Article Snippet: Other (tissue-clearing reagent) , CUBIC-L , Tokyo Chemical Industry , Cat:T3740 , used for tissue clearing of the mouse brain section.



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Image Search Results


Journal: STAR Protocols

Article Title: Determining glioma cell invasion and proliferation in ex vivo organotypic mouse brain slices using whole-mount immunostaining and tissue clearing

doi: 10.1016/j.xpro.2022.101703

Figure Lengend Snippet:

Article Snippet: The described protocol is an adaptation of the whole-mount immunostaining protocol developed by ( ) and the RapiClear 1.47 clearing method developed by the SunJin Lab ( ; ).

Techniques: Recombinant, Fluorsave, Software, Microscopy