Oligomer Restriction:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Immunohistochemistry:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Incubation:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Blocking Assay:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Staining:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Plasmid Preparation:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Activity Assay:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Saline:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
Formalin-fixed Paraffin-Embedded:Article Title: COVID-19 triggers pulmonary tumor outgrowth through hypoxia- and inflammation-induced VEGF-dependent angiogenesis
Article Snippet: Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).Briefly, paraffin-embedded tissues were sectioned at 5 μm, deparaffinized in Tissue-Clear (1466, Sakura), and rehydrated through graded ethanol (99%, 95%, and 70%).. Antigen retrieval was performed by boiling the sections for 20 min in unmasking solution (H3300, Vector).. After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9 (1:300, NB100-417, Novus Biologicals), HIF1α (1:200, NB100-449, Novus Biologicals), F4/80 (1:200, 14-4801-82, Invitrogen), CD80 (1:200, 14-0081-82, Invitrogen), CD206 (1:200, 141702, Biolegend), CD3 (1:100, 17-0032-80, Thermo Fisher Scientific), CD4 (1:100, 550280, BD Pharmingen).After blocking with 4% serum at room temperature, the sections were incubated overnight at 4 °C with primary antibodies against CD31 (1:100, 553370, BD Pharmingen), CD163 (1:100; 14-1631-82, Thermo Fisher Scientific), EpCAM (1:100; 14-5791-81, Thermo Fisher Scientific), CD86 (1:100, 19589, Cell Signaling), FAP (1:200, MAB9727, R&D Systems), α-SMA (1:200, MA5-11547, Thermo Fisher Scientific), desmin (1:200, MAB3430, Millipore), CA9..
Article Title: Constitutive neuronal expression and disease-associated upregulation of chitinases in amyotrophic lateral sclerosis
Article Snippet: Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).Paraffin-embedded sections of 4 μm thickness were first deparaffinized using 100% RotiHistol (2×, 5 min) and then rehydrated along an ethanol gradient (100% twice, 96%, 80%, 70%; 1 min each).. Antigen retrieval was performed by boiling for 10 min in preheated antigen unmasking solution (Vector Laboratories, H-3300, 1:100).. Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.Sections were briefly washed in distilled water, and autofluorescence was quenched (EMD Millipore, 2160, 1 min); following this, sections were incubated in freshly prepared 3% H 2 O 2 for 10 min.
Article Title: A canonical role of SMAD4 in safeguarding 3D genome architecture to suppress lung tumorigenesis.
Article Snippet: sin (Solarbio, G1100). Enhanced iron staining was performed using a Prussian Blue Staining kit (Solarbio, G1428). For IHC, antigen retrieval was conducted by microwaving in Antigen Unmasking Solution (Vector Laboratories, H-3300-250) or 1× TE Buffer at 100 °C for 15min. Endogenous peroxidase activity was blocked with an IHC Kit (ZSGB Bio, PV-6000), followed by 1 h of blocking in 1% BSA at room temp
Article Title: Combined disulfiram and MK-8776 treatment reveals cell line–dependent antitumor activity in different 3D ovarian cancer subtypes
Article Snippet: AR TIC LE IN PR ES S ARTICLE IN PRESS retrieval was performed using Antigen Unmasking Solution, Citrate-Based (H-3300-250, Vector Laboratories), for 30 min at 95 °C.. The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.The protocol was then divided into immunohistochemical (IHC) and immunofluorescence (IF) staining workflows.
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