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citrate buffer  (Thermo Fisher)


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    Structured Review

    Thermo Fisher citrate buffer
    Citrate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/citrate+buffer/Buffer+solution+pH+4%2C+Citrate+buffer/pm41697422-89-2-6
    Average 96 stars, based on 1 article reviews
    citrate buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Stereochemical identity of lipid nanoparticles modulates protein expression via internal lipid organization
    Article Snippet: TriLink CleanCap® 5-methoxyuridine (5moU) modified EGFP-mRNA and CleanCap® AG Reagent were purchased from Tebubio (Heerhugowaard, The Netherlands). .. Quant-ITTM RiboGreen RNA Assay Kit, 0.5 M Citrate Buffer (pH 4.0), Invitrogen reagents for IVT reactions, IVT purification, and agarose gel staining, and Gibco supplements for culture media were purchased from Fisher Emergo B.V. (Landsmeer, The Netherlands). .. DPBS (10×) was obtained from VWR International B.V. (Amsterdam, The Netherlands).

    Article Title: A single-nucleotide change in the Kozak sequence enhances protein expression
    Article Snippet: Afterward, 1 μL of DNase I-XT (NEB) per μg of template DNA was added to the reaction, which was then incubated for 15 min at 37 °C in a thermal cycler. .. Immediately thereafter, the IVT reaction was purified using the Monarch ® Spin RNA Cleanup kit (50 or 500 μg; NEB) and eluted into 1 mM citrate buffer, pH 6.4 (Invitrogen), and kept on ice. .. The integrity and purity of 100 ng of purified IVT mRNA were verified using a 2% agarose gel stained with SYBR TM Gold (Invitrogen) and the High Range ssRNA ladder (NEB), both diluted in the supplied 2× RNA loading dye, following the manufacturer’s sample preparation recommendations.

    Agarose Gel Electrophoresis:

    Article Title: Stereochemical identity of lipid nanoparticles modulates protein expression via internal lipid organization
    Article Snippet: TriLink CleanCap® 5-methoxyuridine (5moU) modified EGFP-mRNA and CleanCap® AG Reagent were purchased from Tebubio (Heerhugowaard, The Netherlands). .. Quant-ITTM RiboGreen RNA Assay Kit, 0.5 M Citrate Buffer (pH 4.0), Invitrogen reagents for IVT reactions, IVT purification, and agarose gel staining, and Gibco supplements for culture media were purchased from Fisher Emergo B.V. (Landsmeer, The Netherlands). .. DPBS (10×) was obtained from VWR International B.V. (Amsterdam, The Netherlands).

    Staining:

    Article Title: Stereochemical identity of lipid nanoparticles modulates protein expression via internal lipid organization
    Article Snippet: TriLink CleanCap® 5-methoxyuridine (5moU) modified EGFP-mRNA and CleanCap® AG Reagent were purchased from Tebubio (Heerhugowaard, The Netherlands). .. Quant-ITTM RiboGreen RNA Assay Kit, 0.5 M Citrate Buffer (pH 4.0), Invitrogen reagents for IVT reactions, IVT purification, and agarose gel staining, and Gibco supplements for culture media were purchased from Fisher Emergo B.V. (Landsmeer, The Netherlands). .. DPBS (10×) was obtained from VWR International B.V. (Amsterdam, The Netherlands).

    Incubation:

    Article Title: Enhancing soil health with Bacillus velezensis UFV 3918 boosts phosphorus and nutrient accumulation in sugarcane
    Article Snippet: .. Soil samples of 1 g were incubated with citrate buffer (pH 6.7) and a 10% urea solution at 37 °C for 2 h. The ammonium nitrogen (N–NH 4 ) released during the reaction was quantified at 600 nm using a spectrophotometer (Thermo Fisher Scientific, BioMateTM 3, Waltham, MA, USA) with the commercial Urea 500 ® kit (Doles Inc., Goiânia, GO, Brazil). ..

    Article Title: Empagliflozin mitigated cisplatin induced renal endothelial dysfunction in rats via repressing oxidative, inflammatory, apoptotic and fibrotic signals.
    Article Snippet: .. For heat-induced epitope retrieval, slides were immersed in citrate buffer (pH 6, Thermo Scientific, Berlin, Germany) then incubated in the microwave at power 100 and 30 for 1 and 9 min, respectively. ..

    Article Title: Comparative evaluation of leucine-rich repeat kinase 2 and isthmin-1 immunoreactivity in malignant mesothelioma and reactive mesothelial hyperplasia
    Article Snippet: .. Following rehydration, antigen retrieval was conducted by microwave heating in citrate buffer (pH 6) at 750 W. After washing with PBS, the sections were incubated in hydrogen peroxide blocking solution for 5 minutes, followed by treatment with Ultra V Block (TA-125-UB; Lab Vision Corp.) for another 5 minutes. .. Primary antibodies applied included ISM-1 (1:200; E-AB-18133, Elabscience Biotechnology, USA) and LRRK2 (orb500678; Biorbyt Ltd., Cambridge, UK).

    Spectrophotometry:

    Article Title: Enhancing soil health with Bacillus velezensis UFV 3918 boosts phosphorus and nutrient accumulation in sugarcane
    Article Snippet: .. Soil samples of 1 g were incubated with citrate buffer (pH 6.7) and a 10% urea solution at 37 °C for 2 h. The ammonium nitrogen (N–NH 4 ) released during the reaction was quantified at 600 nm using a spectrophotometer (Thermo Fisher Scientific, BioMateTM 3, Waltham, MA, USA) with the commercial Urea 500 ® kit (Doles Inc., Goiânia, GO, Brazil). ..

    Blocking Assay:

    Article Title: Comparative evaluation of leucine-rich repeat kinase 2 and isthmin-1 immunoreactivity in malignant mesothelioma and reactive mesothelial hyperplasia
    Article Snippet: .. Following rehydration, antigen retrieval was conducted by microwave heating in citrate buffer (pH 6) at 750 W. After washing with PBS, the sections were incubated in hydrogen peroxide blocking solution for 5 minutes, followed by treatment with Ultra V Block (TA-125-UB; Lab Vision Corp.) for another 5 minutes. .. Primary antibodies applied included ISM-1 (1:200; E-AB-18133, Elabscience Biotechnology, USA) and LRRK2 (orb500678; Biorbyt Ltd., Cambridge, UK).



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