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circular plasmids puc57 encoding the gene of target mrna  (GenScript corporation)

 
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    GenScript corporation circular plasmids puc57 encoding the gene of target mrna
    Circular Plasmids Puc57 Encoding The Gene Of Target Mrna, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circular+encoder/puc57/pm39400114-71-5-12
    Average 90 stars, based on 1 article reviews
    circular plasmids puc57 encoding the gene of target mrna - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Compositions and methods for treating, preventing or reversing age-associated inflammation and disorder
    Article Snippet: Methods Biochemical Assay The biochemical assay was based on Feng et al. (1996)xxxvii and Repanas et al. (2007).xxxviii Untagged human L1 EN domain (1-239) was purified from E. coli using heparin, cation-exchange, and size exclusion chromatography as previously described by Weichenrieder et al. (2004).xxxix Substrate supercoiled plasmid for the biochemical assay, pUC57, containing the E. coli codon-optimized ORF0 sequence (GenScript), was prepared using a QIAPrep Spin Miniprep Kit (Qiagen).

    Article Title: Supporting Information Selecting Better Biocatalysts by Complementing Recoded Bacteria
    Article Snippet: Plasmid pUC57-3iYaaRS, bearing the gene encoding the 3iY aminoacyl tRNA synthetase (3iYaaRS)[4], the 2MCS synthetic gBlock fragment and the SmLcar coding sequence were purchased from GenScript (USA); pACYCDuet-1 was purchased from MilliporeSigma (Novagen), pULTRA-CNF was a gift from Prof. Dr. Peter Schultz (Addgene plasmid #48215).

    Article Title:
    Article Snippet: The vectors pUC57-[EMCV nt.373-1656] for transcription of wt and mutant EMCV IRES-containing mRNA were made by GenScript and contained a T7 promoter followed by EMCV nt.373-1656, with or without mutations as indicated in the text.

    Article Title: KLF5 controls subtype-independent highly interactive enhancers in pancreatic cancer to regulate cell survival
    Article Snippet: Custom constructs were synthesized and cloned into a pUC57-mini backbone from GenScript.

    Article Title: HMO production
    Article Snippet: In similar ways backbones based on pUC57 (GeneScript) or an any other appropriated vector containing two I-SceI endonuclease sites, separated by two DNA fragments appropriated for homologous recombination into the E. coli genome and a T1 transcriptional terminator sequence could be synthesized.

    Plasmid Preparation:

    Article Title: nuTCRacker: Predicting the Recognition of HLA-I-Peptide Complexes by αβTCRs for Unseen Peptides.
    Article Snippet: .. Codon-optimised sequences were synthesised in a pUC57 plasmid (Genscript, UK), and subsequently cloned into a transfer plasmid belonging to a 2nd generation lentiviral system. ..

    Article Title:
    Article Snippet: .. plasmid pUC57 FusX, containing a ‘FusX cassette’, was obtained through a gene synthesis service (GenScript). .. The FusX cassette consists of a fragment of the canonical TALE N-terminal domain (TALE NT- T), a BsmBI restriction site, the components necessary for blue-white screening (i.e., CAP binding site–lac promoter–lac operator–lacZα), an additional BsmBI restriction site, and a fragment of the TALE C- terminal domain.

    Article Title: HMO production
    Article Snippet: Colony PCR was done using DreamTaq PCR Master Mix in a T100 Thermal Cycler (Bio-Rad). .. For example in one plasmid backbone the gal operon (required for homologous recombination in galk), and a T1 transcriptional terminator sequence (pUC57::gal) was synthesized (GeneScript). ..

    Clone Assay:

    Article Title: nuTCRacker: Predicting the Recognition of HLA-I-Peptide Complexes by αβTCRs for Unseen Peptides.
    Article Snippet: .. Codon-optimised sequences were synthesised in a pUC57 plasmid (Genscript, UK), and subsequently cloned into a transfer plasmid belonging to a 2nd generation lentiviral system. ..

    Homologous Recombination:

    Article Title: HMO production
    Article Snippet: Colony PCR was done using DreamTaq PCR Master Mix in a T100 Thermal Cycler (Bio-Rad). .. For example in one plasmid backbone the gal operon (required for homologous recombination in galk), and a T1 transcriptional terminator sequence (pUC57::gal) was synthesized (GeneScript). ..

    Sequencing:

    Article Title: HMO production
    Article Snippet: Colony PCR was done using DreamTaq PCR Master Mix in a T100 Thermal Cycler (Bio-Rad). .. For example in one plasmid backbone the gal operon (required for homologous recombination in galk), and a T1 transcriptional terminator sequence (pUC57::gal) was synthesized (GeneScript). ..

    Synthesized:

    Article Title: HMO production
    Article Snippet: Colony PCR was done using DreamTaq PCR Master Mix in a T100 Thermal Cycler (Bio-Rad). .. For example in one plasmid backbone the gal operon (required for homologous recombination in galk), and a T1 transcriptional terminator sequence (pUC57::gal) was synthesized (GeneScript). ..



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