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m6a-circrna epitranscriptomic microarray  (Arraystar inc)

 
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    Arraystar inc m6a-circrna epitranscriptomic microarray
    Differentially expressed <t>m6A</t> modified circRNAs and mRNA. (A) Hierarchical clustering heatmap of differentially m6A modified circRNAs. (B) Volcano plots of differentially mRNA based on TCGA-COAD. (C) Volcano plots of differentially mRNA based on GSE106582. (D) The intersected genes between <t>microarray,</t> COAD-TCGA and GSE106582.
    M6a Circrna Epitranscriptomic Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circrna+microarray+arraystar+mouse+circrna+array+v2/m6a+circrna+epitranscriptomic+microarray/pmc09373052-31-1-0
    Average 90 stars, based on 1 article reviews
    m6a-circrna epitranscriptomic microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Microarray and bioinformatic analysis reveal the parental genes of m6A modified circRNAs as novel prognostic signatures in colorectal cancer"

    Article Title: Microarray and bioinformatic analysis reveal the parental genes of m6A modified circRNAs as novel prognostic signatures in colorectal cancer

    Journal: Frontiers in Oncology

    doi: 10.3389/fonc.2022.939790

    Differentially expressed m6A modified circRNAs and mRNA. (A) Hierarchical clustering heatmap of differentially m6A modified circRNAs. (B) Volcano plots of differentially mRNA based on TCGA-COAD. (C) Volcano plots of differentially mRNA based on GSE106582. (D) The intersected genes between microarray, COAD-TCGA and GSE106582.
    Figure Legend Snippet: Differentially expressed m6A modified circRNAs and mRNA. (A) Hierarchical clustering heatmap of differentially m6A modified circRNAs. (B) Volcano plots of differentially mRNA based on TCGA-COAD. (C) Volcano plots of differentially mRNA based on GSE106582. (D) The intersected genes between microarray, COAD-TCGA and GSE106582.

    Techniques Used: Modification, Microarray

    Information on circRNAs formed by nine parental genes.
    Figure Legend Snippet: Information on circRNAs formed by nine parental genes.

    Techniques Used:

    Related Articles

    Microarray:

    Article Title: WTAP-Mediated N6-Methyladenosine of RNAs Facilitate the Pathophysiology of Atopic Dermatitis.
    Article Snippet: 1058 WTAP-Mediated N6-Methyladenosine of RNAs Facilitate the Pathophysiology of Atopic Dermatitis Lina Tan, Xue Chen, Siyu Yan, Aiyuan Guo, Lihua Gao, Lu Zhou, Yanping Zhou, Jinrong Zeng and Jianyun Lu N6-methyladenosine (mA) is the most abundant dynamic and reversible internal chemical modification of RNA in eukaryotic cells and is essential in multiple pathophysiological processes.. However, it has not been reported in atopic dermatitis (AD).. We used Arraystar mAemRNA epitranscriptomic microarray to screen for differentially expressed genes and their mA levels and mA-related enzymes in patients with AD.

    Article Title: Morphine-Driven m6A Epitranscriptomic Neuroadaptations in Primary Cortical Cultures.
    Article Snippet: .. Cells were transfected on div 7 and harvested after 72 h on div 10. m6A Epitranscriptomic Microarray Analysis Evaluation of m6A hyper- and hypomethylation in RNA samples from primary cortical cultures was performed by Arraystar Inc. (Rockville, MD, USA), as previously described [29]. ..

    Transfection:

    Article Title: Morphine-Driven m6A Epitranscriptomic Neuroadaptations in Primary Cortical Cultures.
    Article Snippet: .. Cells were transfected on div 7 and harvested after 72 h on div 10. m6A Epitranscriptomic Microarray Analysis Evaluation of m6A hyper- and hypomethylation in RNA samples from primary cortical cultures was performed by Arraystar Inc. (Rockville, MD, USA), as previously described [29]. ..

    other:

    Article Title: The m7G Methyltransferase Mettl1 Drives Cardiac Hypertrophy by Regulating SRSF9-Mediated Splicing of NFATc4.
    Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense Arraystar m7G-mRNA Epitranscriptomic Microarray: Total RNA from each sample was quantified using the NanoDrop ND-1000 and RNA integrity was assessed by Bioanalyzer 2100 or Mops electrophoresis.

    Article Title: Circular RNA circDhx32 promotes cardiac inflammatory responses in mouse cardiac ischemia-reperfusion injury via binding to FOXO1 competed with AdipoR1.
    Article Snippet: The circDhx32 and GAPDH expression levels were subsequently determined by qRT‒PCR. m6A-circRNA epitranscriptomic microarray and bioinformatic analysis Sample preparation and microarray hybridization were performed according to Arraystar’s standard protocols.

    Methylation:

    Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway.
    Article Snippet: .. In this research, we applied Arraystar m6A lncRNA epitranscriptomic microarrays to screen differentially m6Amethylated lncRNAs in lung cancer and adjacent tissues and then identified the priority candidate genes SCIRT. m6A methylation and SCIRT expression were upregulated in a METTL3-dependent manner in NSCLC cells. ..

    Expressing:

    Article Title: M 6 A -mediated lncRNA SCIRT stability promotes NSCLC progression through binding to SFPQ and activating the PI3K/Akt pathway.
    Article Snippet: .. In this research, we applied Arraystar m6A lncRNA epitranscriptomic microarrays to screen differentially m6Amethylated lncRNAs in lung cancer and adjacent tissues and then identified the priority candidate genes SCIRT. m6A methylation and SCIRT expression were upregulated in a METTL3-dependent manner in NSCLC cells. ..



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    circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)  (Arraystar inc)
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    Arraystar inc circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)
    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show <t>circRNA</t> expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.
    Circrna Expression Microarray Slide Arraystar Mouse Circrna Array V2 (8 × 15k), supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    circrna microarray arraystar mouse circrna array v2  (Arraystar inc)
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    Arraystar inc circrna microarray arraystar mouse circrna array v2
    The primers used in qRT-PCR experiments.
    Circrna Microarray Arraystar Mouse Circrna Array V2, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circrna+microarray+arraystar+mouse+circrna+array+v2/circrna+microarray+arraystar+human+circrna+array+v2/pmc06697070-120-1-6
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Expressing, Control, Quantitative Proteomics

    RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Quantitative RT-PCR, Microarray, Quantitative Proteomics

    circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    The primers used in qRT-PCR experiments.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: The primers used in qRT-PCR experiments.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray

    DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques: