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circrna microarray  (Arraystar inc)

 
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    Arraystar inc circrna microarray
    Top 10 differentially expressed circRNAs (degenerated vs. non-degenerated)
    Circrna Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circrna+microarray+arraystar+mouse+circrna+array+v2/pmc05938034-67-7-9?v=Arraystar+inc
    Average 90 stars, based on 1 article reviews
    circrna microarray - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "Circular RNA circ-4099 is induced by TNF-α and regulates ECM synthesis by blocking miR-616-5p inhibition of Sox9 in intervertebral disc degeneration"

    Article Title: Circular RNA circ-4099 is induced by TNF-α and regulates ECM synthesis by blocking miR-616-5p inhibition of Sox9 in intervertebral disc degeneration

    Journal: Experimental & Molecular Medicine

    doi: 10.1038/s12276-018-0056-7

    Top 10 differentially expressed circRNAs (degenerated vs. non-degenerated)
    Figure Legend Snippet: Top 10 differentially expressed circRNAs (degenerated vs. non-degenerated)

    Techniques Used:

    a Scatter plot of circRNA expression. The circRNAs above the top green line and below the bottom green line indicated more than a twofold change between the degenerated and normal IVD NP tissues. b Volcano Plot of the differentially expressed circRNAs. The red points in the plot represent differentially expressed circRNAs with statistical significance. c Hierarchical clustering shows the distinguishable circRNA expression profile between the two groups and homogeneity within the groups. d The sequence of the amplified Circ-4099 product was completely consistent with CircBase. e Co-localization between circ-4099 (red) and miR-616-5p (green) was observed by RNA in situ hybridization in hNP cells after co-transfection with circ-4099-expressing and miR-616-5p-expressing vectors. The nuclei were stained with DAPI (blue). Scale bar = 10 µm. f The expression levels of circ-4099 are significantly higher in degenerated NP tissues than in non-degenerated NP tissues. g – i The expression levels of circ-68610/2069/92328 are higher in degenerated NP tissues than in non-degenerated NP tissues. j qRT-PCR analysis shows that TNF-α upregulates the expression of circ-4099 in a dose-dependent and time-dependent manner. For time-dependent studies, 50 ng/ml TNF-α was used. k hNP cells were transfected with the circ-4099-Luc promoter and the induction in luciferase activity was measured following TNF-α treatment ( P < 0.001)
    Figure Legend Snippet: a Scatter plot of circRNA expression. The circRNAs above the top green line and below the bottom green line indicated more than a twofold change between the degenerated and normal IVD NP tissues. b Volcano Plot of the differentially expressed circRNAs. The red points in the plot represent differentially expressed circRNAs with statistical significance. c Hierarchical clustering shows the distinguishable circRNA expression profile between the two groups and homogeneity within the groups. d The sequence of the amplified Circ-4099 product was completely consistent with CircBase. e Co-localization between circ-4099 (red) and miR-616-5p (green) was observed by RNA in situ hybridization in hNP cells after co-transfection with circ-4099-expressing and miR-616-5p-expressing vectors. The nuclei were stained with DAPI (blue). Scale bar = 10 µm. f The expression levels of circ-4099 are significantly higher in degenerated NP tissues than in non-degenerated NP tissues. g – i The expression levels of circ-68610/2069/92328 are higher in degenerated NP tissues than in non-degenerated NP tissues. j qRT-PCR analysis shows that TNF-α upregulates the expression of circ-4099 in a dose-dependent and time-dependent manner. For time-dependent studies, 50 ng/ml TNF-α was used. k hNP cells were transfected with the circ-4099-Luc promoter and the induction in luciferase activity was measured following TNF-α treatment ( P < 0.001)

    Techniques Used: Expressing, Sequencing, Amplification, RNA In Situ Hybridization, Cotransfection, Staining, Quantitative RT-PCR, Transfection, Luciferase, Activity Assay

    a Plasmid map of the Circ-4099 over-expression plasmid. b The qPCR products were used to confirm the Circ-4099 sequence. c , d hNP cells were transfected with Circ-4099, NC (PLCDH-ciR) or negative control, and total RNA was collected. qPCR was used to detect the expression of Circ-4099 and its linear RNA (DENND5A) (linear + circRNA). The results showed that the Circ-4099 over-expression plasmid can extremely enhance Circ-4099 expression. These data confirm the validity and efficiency of the Circ-4099 over-expression plasmid. e , f qRT-PCR showed that Circ-4099 over-expression led to increased Collagen II and Aggrecan mRNA expression, which was suppressed by treatment with miR-616-5p mimics, while MMP3 and ADAMTS5 showed few significant differences in regulation. g , h Western blotting also showed that he Collagen II and Aggrecan protein expression levels were significantly increased by circ-4099 over-expression treatment. We next tested the effects of Circ-4099 on expression of inflammatory cytokines in hNP. i , j The IL-1β, TNF-α, and PGE2 mRNA levels were significantly suppressed in hNP cells transduced with Circ-4099 over-expression plasmid, which was inhibited by treatment with miR-616-5p mimics. k , l IL-1β, TNF-α, IL-6, and PGE2 cytokine release into medium was detected by ELISA, which showed similar results
    Figure Legend Snippet: a Plasmid map of the Circ-4099 over-expression plasmid. b The qPCR products were used to confirm the Circ-4099 sequence. c , d hNP cells were transfected with Circ-4099, NC (PLCDH-ciR) or negative control, and total RNA was collected. qPCR was used to detect the expression of Circ-4099 and its linear RNA (DENND5A) (linear + circRNA). The results showed that the Circ-4099 over-expression plasmid can extremely enhance Circ-4099 expression. These data confirm the validity and efficiency of the Circ-4099 over-expression plasmid. e , f qRT-PCR showed that Circ-4099 over-expression led to increased Collagen II and Aggrecan mRNA expression, which was suppressed by treatment with miR-616-5p mimics, while MMP3 and ADAMTS5 showed few significant differences in regulation. g , h Western blotting also showed that he Collagen II and Aggrecan protein expression levels were significantly increased by circ-4099 over-expression treatment. We next tested the effects of Circ-4099 on expression of inflammatory cytokines in hNP. i , j The IL-1β, TNF-α, and PGE2 mRNA levels were significantly suppressed in hNP cells transduced with Circ-4099 over-expression plasmid, which was inhibited by treatment with miR-616-5p mimics. k , l IL-1β, TNF-α, IL-6, and PGE2 cytokine release into medium was detected by ELISA, which showed similar results

    Techniques Used: Plasmid Preparation, Over Expression, Sequencing, Transfection, Negative Control, Expressing, Quantitative RT-PCR, Western Blot, Transduction, Enzyme-linked Immunosorbent Assay



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    circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)  (Arraystar inc)
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    Arraystar inc circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k)
    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show <t>circRNA</t> expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.
    Circrna Expression Microarray Slide Arraystar Mouse Circrna Array V2 (8 × 15k), supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circrna+microarray+arraystar+mouse+circrna+array+v2/pmc07015875-65-15-19?v=Arraystar+inc
    Average 90 stars, based on 1 article reviews
    circrna expression microarray slide arraystar mouse circrna array v2 (8 × 15k) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    circrna microarray arraystar mouse circrna array v2  (Arraystar inc)
    90
    Arraystar inc circrna microarray arraystar mouse circrna array v2
    The primers used in qRT-PCR experiments.
    Circrna Microarray Arraystar Mouse Circrna Array V2, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circrna+microarray+arraystar+mouse+circrna+array+v2/pmc06697070-120-1-6?v=Arraystar+inc
    Average 90 stars, based on 1 article reviews
    circrna microarray arraystar mouse circrna array v2 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Expressing, Control, Quantitative Proteomics

    RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Quantitative RT-PCR, Microarray, Quantitative Proteomics

    circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    The primers used in qRT-PCR experiments.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: The primers used in qRT-PCR experiments.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: Top 10 up- and down-regulated DEcircRNAs in the hippocampus of Nrf2 (−/−) mice.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques:

    QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: QRT-PCR validation of the expression levels of candidate circRNAs (A) and lncRNAs (B) . * p < 0.05 and ∗∗ p < 0.01. The deep red column indicates the expression status of lncRNAs through microarray analyses; the blue column indicates the expression status of lncRNAs through qRT-PCR experiments. n = 3.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques: Quantitative RT-PCR, Biomarker Discovery, Expressing, Microarray

    DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

    Journal: Frontiers in Molecular Neuroscience

    Article Title: Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

    doi: 10.3389/fnmol.2019.00196

    Figure Lengend Snippet: DEcircRNA-miRNA-DEceRNA interaction subnetworks of up-regulated circRNAs and down-regulated circRNAs in the Nrf2 (–/–) hippocampus. (A) Subnetwork of mmu_circRNA_44531 in the Nrf2 (–/–) hippocampus. (B) Subnetwork of mmu_circRNA_34132 in the Nrf2 (–/–) hippocampus. (C) Subnetwork of mmu_circRNA_000903 in the Nrf2 (–/–) hippocampus. (D) Subnetwork of mmu_circRNA_018676 in the Nrf2 (–/–) hippocampus. (E) Subnetwork of mmu_circRNA_45901 in the Nrf2 (–/–) hippocampus. (F) Subnetwork of mmu_circRNA_33836 in the Nrf2 (–/–) hippocampus. (G) Subnetwork of mmu_circRNA_34137 in the Nrf2 (–/–) hippocampus. (H) Subnetwork of mmu_circRNA_34106 in the Nrf2 (–/–) hippocampus. (I) Subnetwork of mmu_circRNA_008691 in the Nrf2 (–/–) hippocampus. (J) Subnetwork of mmu_circRNA_003237 in the Nrf2 (–/–) hippocampus. Yellow nodes indicate DEcircRNAs. Magenta and green nodes indicate miRNAs sponged by DEcircRNAs and the gene ID of their DEceRNAs, respectively. Edges represent interactions.

    Article Snippet: The circRNA microarray was analyzed using Arraystar Mouse circRNA Array V2 analysis (Arraystar, Inc., United States) by Kangchen BioTech, Inc. (Shanghai, China).

    Techniques: