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human circrna microarray slides v2.0  (Arraystar inc)

 
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    Arraystar inc human circrna microarray slides v2.0
    Differentially expressed circRNAs in the plasma of ESCC patients.
    Human Circrna Microarray Slides V2.0, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/circrna+expression+microarray+slides/human+circrna+array+v2/pmc07966720-101-19-27
    Average 90 stars, based on 1 article reviews
    human circrna microarray slides v2.0 - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma"

    Article Title: Systematic Identification of circRNA–miRNA–mRNA Regulatory Network in Esophageal Squamous Cell Carcinoma

    Journal: Frontiers in Genetics

    doi: 10.3389/fgene.2021.580390

    Differentially expressed circRNAs in the plasma of ESCC patients.
    Figure Legend Snippet: Differentially expressed circRNAs in the plasma of ESCC patients.

    Techniques Used: Clinical Proteomics

    Differentially expressed circRNAs in ESCC patients. (A) The scatter plot of differentially expressed circRNAs. Green lines represent fold change of 2.0. (B) Volcano plot of differentially expressed circRNAs. The green vertical lines correspond to a fold change of 2.0, and the green horizontal line corresponds to a P -value of 0.05. (C) Hierarchical clustering of differentially expressed circRNAs. Each column represents a sample and each row represents a circRNA. The color reflects the expression level of circRNAs and changes from red (high) to black (medium) to green (low). (D) Bar plot shows the distributions of the differentially expressed circRNAs in human chromosomes.
    Figure Legend Snippet: Differentially expressed circRNAs in ESCC patients. (A) The scatter plot of differentially expressed circRNAs. Green lines represent fold change of 2.0. (B) Volcano plot of differentially expressed circRNAs. The green vertical lines correspond to a fold change of 2.0, and the green horizontal line corresponds to a P -value of 0.05. (C) Hierarchical clustering of differentially expressed circRNAs. Each column represents a sample and each row represents a circRNA. The color reflects the expression level of circRNAs and changes from red (high) to black (medium) to green (low). (D) Bar plot shows the distributions of the differentially expressed circRNAs in human chromosomes.

    Techniques Used: Expressing

    CircRNA–miRNA–mRNA interaction network in ESCC. CircRNAs are represented by diamonds; miRNAs are shown as triangles; the DEGs are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA; ESCC, esophageal squamous cell carcinoma; DEGs, differentially expressed genes.
    Figure Legend Snippet: CircRNA–miRNA–mRNA interaction network in ESCC. CircRNAs are represented by diamonds; miRNAs are shown as triangles; the DEGs are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA; ESCC, esophageal squamous cell carcinoma; DEGs, differentially expressed genes.

    Techniques Used: Expressing

    Sub-network of circRNAs, miRNAs, and hub genes. circRNAs are represented by diamonds; miRNAs are shown as triangles; hub genes are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA.
    Figure Legend Snippet: Sub-network of circRNAs, miRNAs, and hub genes. circRNAs are represented by diamonds; miRNAs are shown as triangles; hub genes are represented by ellipses. Red represents upregulated expression, and blue color represents downregulated expression. circRNA, circular RNA; miRNA, microRNA.

    Techniques Used: Expressing

    Related Articles

    Labeling:

    Article Title: The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.
    Article Snippet: Using random primers as per the Super RNA Labeling protocol by Arraystar Inc., the total RNA from each specimen was amplified and then converted into fluorescent cRNA (Arraystar Inc.). .. The labeled cRNAs were then hybridized to the Arraystar Human circRNA Array. .. After the washing steps, an Axon GenePix 4000B microarray scanner was used to scan the arrays.

    Article Title: Activation of the circAGFG1/miR-195-5p/PD-L1 axis induces lung injury in sepsis.
    Article Snippet: The enriched circular RNAs were then amplified and transcribed into fluorescent cRNA using a random priming method provided by the Arraystar Super RNA Labeling Kit. .. The labeled cRNAs were subsequently hybridized onto the Arraystar Human circRNA Array V2 (8 × 15 K, Arraystar). .. Following slide washing, the arrays were scanned using the Agilent Scanner G2505C.

    Significance Assay:

    Article Title: Interferon-stimulated circHOMER1 attenuates antiviral innate immunity.
    Article Snippet: .. Using the human CircRNA Array V2 (8 × 15K, Arraystar) Month XXXX Volume 0 Issue 0 10.1128/mbio.01497-25 2 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/m bi o on 1 7 Ju ly 2 02 5 by 2 a0 9: ba c1 :3 4c 0: 18 ::1 f1 :2 11 . with a total of 10,926 circRNA probes, we identified 82 upregulated and 20 downregula ted circRNAs with a threshold of P-value < 0.05 and a fold change cutoff of 2 by volcano plots (Fig. 1A) and heatmap (Fig. 1B). ..

    Expressing:

    Article Title: Circular RNA TFRC/ SCD1 mRNA interaction regulates ferroptosis and metastasis in gastric cancer
    Article Snippet: .. CircRNA expression profiling was performed using the Arraystar Human CircRNA microarray V1 ( GSE93541 , GSE83521 ) and V2 ( GSE194384 ) on GPL19978 and GPL21825 platforms, respectively. ..

    Article Title: Circular RNA TFRC/SCD1 mRNA interaction regulates ferroptosis and metastasis in gastric cancer.
    Article Snippet: .. CircRNA expression profiling was performed using the Arraystar Human CircRNA microarray V1 (GSE93541, GSE83521) and V2 (GSE194384) on GPL19978 and GPL21825 platforms, respectively. ..

    Microarray:

    Article Title: Circular RNA TFRC/ SCD1 mRNA interaction regulates ferroptosis and metastasis in gastric cancer
    Article Snippet: .. CircRNA expression profiling was performed using the Arraystar Human CircRNA microarray V1 ( GSE93541 , GSE83521 ) and V2 ( GSE194384 ) on GPL19978 and GPL21825 platforms, respectively. ..

    Article Title: Circular RNA TFRC/SCD1 mRNA interaction regulates ferroptosis and metastasis in gastric cancer.
    Article Snippet: .. CircRNA expression profiling was performed using the Arraystar Human CircRNA microarray V1 (GSE93541, GSE83521) and V2 (GSE194384) on GPL19978 and GPL21825 platforms, respectively. ..

    Spectrophotometry:

    Article Title: The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.
    Article Snippet: .. METHODS Arraystar human circRNA array analysis Each sample’s total RNA was measured with a NanoDrop ND-1000 spectrophotometer. .. The guidelines given by Arraystar dictated the steps for sample preparation and microarray hybridization.

    other:

    Article Title: The molecular axis hnRNPU/circKCNK2/EDC4/IL-11 aggravates osteolytic bone metastasis of RCC.
    Article Snippet: RESULTS Increased expression of circKCNK2 was associated with RCC progression and bone metastasis Utilizing the Arraystar human circRNA (V2.0) platform, we performed a differential analysis between five RCC-primary tumors Oncogene and five RCC-bone metastases (Bone Met).



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    Image Search Results


    Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

    Journal: The Journal of International Medical Research

    Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

    doi: 10.1177/03000605211024501

    Figure Lengend Snippet: Primer details for quantitative real-time reverse transcription–polymerase chain reaction of circRNA_000585.

    Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Sequencing, Amplification

    Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

    Journal: The Journal of International Medical Research

    Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

    doi: 10.1177/03000605211024501

    Figure Lengend Snippet: Heatmap of circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens (P1–3) and matched para-cancer specimens (C1–3) from three patients with CCA. Each block represents different circRNA expression levels (red represents high expression; green represents low expression).

    Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

    Techniques: RNA Expression, Blocking Assay, Expressing

    Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

    Journal: The Journal of International Medical Research

    Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

    doi: 10.1177/03000605211024501

    Figure Lengend Snippet: Fold change in circular (circ)RNA expression levels in cholangiocarcinoma (CCA) tumour specimens and matched para-cancer specimens from three patients with CCA. (A) scatter plot (dots above the upper line represent fold-change >1.5, dots below the lower line represent fold-change >–1.5); and (B) volcano plot of circRNA expression (red dots represent fold-change >1.5 or >–1.5).

    Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

    Techniques: RNA Expression, Expressing

    Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

    Journal: The Journal of International Medical Research

    Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

    doi: 10.1177/03000605211024501

    Figure Lengend Snippet: Vertical scatter plot showing elevated circRNA_000585 expression in cholangiocarcinoma (CCA) tumour tissue versus matched para-cancer tissue from 15 patients with CCA (central horizontal line represents mean, upper and lower horizonal lines represent SD; P = 0.003 between groups).

    Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

    Techniques: Expressing

    Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

    Journal: The Journal of International Medical Research

    Article Title: Potential mechanism of circRNA_000585 in cholangiocarcinoma

    doi: 10.1177/03000605211024501

    Figure Lengend Snippet: Association between circRNA_000585 expression and clinicopathological characteristics in 15 patients with cholangiocarcinoma.

    Article Snippet: The labelled cRNA mixture was diluted by adding 25 μl of 2× hybridization buffer, and 50 μl of the hybridization solution was transferred into the gasket slide, which was then assembled onto the circRNA expression microarray slide (Arraystar Human circRNA Array V2 [8 × 15 K]).

    Techniques: Expressing

    The list of differential expressed circRNAs in plasma exosome samples from patients with GD and healthy control subjects

    Journal: Biological Research

    Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

    doi: 10.1186/s40659-020-00299-y

    Figure Lengend Snippet: The list of differential expressed circRNAs in plasma exosome samples from patients with GD and healthy control subjects

    Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

    Techniques: Clinical Proteomics, Control

    The circRNA-miRNA-mRNA regulatory network of hsa_circRNA_000102. CircRNA, miRNA, and mRNA are indicated as spheres in brown, red, blue color, respectively. CircRNA circular RNA, miRNA microRNA, mRNA messenger RNA

    Journal: Biological Research

    Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

    doi: 10.1186/s40659-020-00299-y

    Figure Lengend Snippet: The circRNA-miRNA-mRNA regulatory network of hsa_circRNA_000102. CircRNA, miRNA, and mRNA are indicated as spheres in brown, red, blue color, respectively. CircRNA circular RNA, miRNA microRNA, mRNA messenger RNA

    Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

    Techniques:

    The top 10 significantly enriched terms of biological process by GO analysis of hsa_circRNA_000102 associated genes. GO analysis was divided into three parts: biological process, cell component and molecular function

    Journal: Biological Research

    Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

    doi: 10.1186/s40659-020-00299-y

    Figure Lengend Snippet: The top 10 significantly enriched terms of biological process by GO analysis of hsa_circRNA_000102 associated genes. GO analysis was divided into three parts: biological process, cell component and molecular function

    Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

    Techniques:

    The top 10 significant enriched pathways by KEGG pathway analysis of hsa_circRNA_000102 associated genes

    Journal: Biological Research

    Article Title: Microarray profiling and functional analysis of differentially expressed plasma exosomal circular RNAs in Graves’ disease

    doi: 10.1186/s40659-020-00299-y

    Figure Lengend Snippet: The top 10 significant enriched pathways by KEGG pathway analysis of hsa_circRNA_000102 associated genes

    Article Snippet: The circRNA expression microarray slide (AS-S-CR-H-V2.0, Arraystar Human circRNA Arrays V2, 8 × 15 K; Arraystar Inc.) was used.

    Techniques:

    Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Characterization of the expression profile of circular RNAs in blood samples of MCAO-treated mice. (A) Normalized intensities of all circular RNAs expressed in the blood in sham and 5 min, 3-h, and 24-h MCAO-treated mice; n = 3 per group. (B) The scatter plots show the differentially expressed circRNAs in the 5-min, 3-h, and 24-h MCAO groups compared with sham. circRNAs in the scatter plot above and below the diagonal line indicate upregulation and downregulation, respectively. (C) Volcano plots show circRNA expression profiles in the 5-min, 3-h, and 24-h MCAO groups compared with sham control. Red dots represent differentially expressed circRNAs ( p < 0.05 and fold-change ≥ 2.0). (D) Distribution of different types of differentially expressed circRNAs, including those consisting of exon, intron, intergenic region, sense, and antisense sequences. (E) Venn diagram shows the overlapping differentially expressed circRNA probes among the three groups compared with sham control. The total numbers of probes exhibiting differential expression in 5 min, 3 h, and 24 h are 1051, 782, and 2721, respectively.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Expressing, Control, Quantitative Proteomics

    RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: RT-qPCR verification of the microarray data from mouse blood. Representative circRNAs with significant differential expression at 5 min (upper panel), 3 h (middle panel), and 24 h of MCAO (lower panel) were verified by RT-qPCR. Left and right panels show the circRNAs with upregulation and downregulation, respectively. Values are mean ± SEM ( n = 3 per group). ∗ p < 0.05, ∗∗ p < 0.05, and ∗∗∗ p < 0.001 compared with sham (independent samples t -test, single-tailed).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: Quantitative RT-PCR, Microarray, Quantitative Proteomics

    circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: circRNA-miRNA interaction. Diagrams show the predicted miRNAs (square boxes) that bind to the verified differentially expressed circRNAs (round circles) at the 5-min (A) , 3-h (B) , and 24-h (C) time points of MCAO in mice. Blue lines represent upregulation; red lines represent downregulation.

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: Gene ontology analysis. Gene ontology classifications of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h, and (C) 24-h time points of MCAO. Color key represents log( p -value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques:

    KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Journal: Frontiers in Neuroscience

    Article Title: Identification of Blood Circular RNAs as Potential Biomarkers for Acute Ischemic Stroke

    doi: 10.3389/fnins.2020.00081

    Figure Lengend Snippet: KEGG pathway analysis of circRNA-miRNA target genes. (A) KEGG pathway analysis of the circRNA-miRNA target genes at the (A) 5-min, (B) 3-h and (C) 24-h time points of MCAO. Color key represents log( p value).

    Article Snippet: Fifty microliters of hybridization solution was dispensed into the gasket slide and assembled on the circRNA expression microarray slide [Arraystar Mouse circRNA Array v2 (8 × 15K, Arraystar)].

    Techniques: