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slc27a5  (Boster Bio)


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    Structured Review

    Boster Bio slc27a5
    The expression and function of <t>SLC27A5</t> in HCC. ( A – C ) The transcriptional expression of SLC27A5 in HCC and normal tissues of TCGA-LIHC ( A ), GSE14520 ( B ) and GSE64041 ( C ) datasets. ( D ) The protein level of SLC27A5 in HCC and normal tissues. ( E , F ) CCK-8 assay for HepG2 ( E ) and LM-3 ( F ) cells overexpressing SL27A5. ( G , H ) HepG2 ( G ) and LM-3 ( H ) cells were subject to flow cytometry analysis for cell cycle. ( I ) HCC cells overexpressing SLC27A5 were subject to EdU staining. ( J ) HCC cells overexpressing SLC27A5 were subject to wound healing assay.
    Slc27a5, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cholate/Anti-SLC27A5%2FFatp5+Antibody/pmc10781498-91-10-12
    Average 93 stars, based on 3 article reviews
    slc27a5 - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis"

    Article Title: Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis

    Journal: Aging (Albany NY)

    doi: 10.18632/aging.205334

    The expression and function of SLC27A5 in HCC. ( A – C ) The transcriptional expression of SLC27A5 in HCC and normal tissues of TCGA-LIHC ( A ), GSE14520 ( B ) and GSE64041 ( C ) datasets. ( D ) The protein level of SLC27A5 in HCC and normal tissues. ( E , F ) CCK-8 assay for HepG2 ( E ) and LM-3 ( F ) cells overexpressing SL27A5. ( G , H ) HepG2 ( G ) and LM-3 ( H ) cells were subject to flow cytometry analysis for cell cycle. ( I ) HCC cells overexpressing SLC27A5 were subject to EdU staining. ( J ) HCC cells overexpressing SLC27A5 were subject to wound healing assay.
    Figure Legend Snippet: The expression and function of SLC27A5 in HCC. ( A – C ) The transcriptional expression of SLC27A5 in HCC and normal tissues of TCGA-LIHC ( A ), GSE14520 ( B ) and GSE64041 ( C ) datasets. ( D ) The protein level of SLC27A5 in HCC and normal tissues. ( E , F ) CCK-8 assay for HepG2 ( E ) and LM-3 ( F ) cells overexpressing SL27A5. ( G , H ) HepG2 ( G ) and LM-3 ( H ) cells were subject to flow cytometry analysis for cell cycle. ( I ) HCC cells overexpressing SLC27A5 were subject to EdU staining. ( J ) HCC cells overexpressing SLC27A5 were subject to wound healing assay.

    Techniques Used: Expressing, CCK-8 Assay, Flow Cytometry, Staining, Wound Healing Assay

    SLC27A5 upregulates FDX1 in HCC. ( A , B ) The correlation between the expression of SLC27A5 and that of FDX1 in TCGA-LIHC ( A ) and GSE14520 ( B ) datasets. ( C ) Dot plot showing the dependency scores for SLC27A5 and FDX1 across all tumor cell lines in the Project Achilles/Cancer Dependency Map Portal (DepMap). ( D , E ) The expression of and correlation between SLC27A5 and FDX1 in collected HCC tissue chip. ( F , G ) HepG2 ( F ) and LM-3 ( G ) cells overexpressing SLC27A5 were subject to qRT-PCR analysis. ( H , I ) Western blotting using lysates of HepG2 ( H ) and LM-3 ( I ) cells after overexpressing SLC27A5.
    Figure Legend Snippet: SLC27A5 upregulates FDX1 in HCC. ( A , B ) The correlation between the expression of SLC27A5 and that of FDX1 in TCGA-LIHC ( A ) and GSE14520 ( B ) datasets. ( C ) Dot plot showing the dependency scores for SLC27A5 and FDX1 across all tumor cell lines in the Project Achilles/Cancer Dependency Map Portal (DepMap). ( D , E ) The expression of and correlation between SLC27A5 and FDX1 in collected HCC tissue chip. ( F , G ) HepG2 ( F ) and LM-3 ( G ) cells overexpressing SLC27A5 were subject to qRT-PCR analysis. ( H , I ) Western blotting using lysates of HepG2 ( H ) and LM-3 ( I ) cells after overexpressing SLC27A5.

    Techniques Used: Expressing, Quantitative RT-PCR, Western Blot

    Related Articles

    Western Blot:

    Article Title: Copper metabolism-related risk score identifies hepatocellular carcinoma subtypes and SLC27A5 as a potential regulator of cuproptosis
    Article Snippet: The BCA kit (P0010, Beyotime) was used to determine the protein concentration. .. Western blotting was performed using antibodies against FDX1 (M05441, Boster), SLC27A5 (A09287-2, Boster), GAPDH (BM3876, Boster). .. Horseradish peroxidase-labeled Goat anti-rabbit IgG (H+L) (BA1039, Boster) was used as secondary antibodies.

    other:

    Article Title: SLC27A5 deficiency activates NRF2/TXNRD1 pathway by increased lipid peroxidation in HCC
    Article Snippet: Anti-SLC27A5 (1:200&1:100, BM2177,BOSTER, China), Anti-β-actin (1:2000, BL005B, Biosharp, China), Anti-TXNRD1 (1:100, GTX108727, Gene Tex, USA), Anti-NRF2 (1:1000&1:200, Ab62352, Abcam, USA), Anti-Lamin B1 (1:10000, AP6001, Bioworld Technology, USA), Anti-β-Tublin (1:5000, 66240-1-Ig, Proteintech Group, USA), Anti-Flag (1:5000&1:300, MA1-91878, Invitrogen, USA), Anti-Myc (1:1000, 2278S, Cell Signaling Technology, USA), Anti-4-HNE (1:3000, Ab46545, Abcam, USA), Anti-GAPDH (1:2000, AG019, Beyotime, China), Anti-Ki67 (1:20, PA516785, Thermo Fisher Scientific, USA), Anti-Bax (1:1000, RLT0456, Ruiying Biological, China), Anti-Bcl2 (1:1000 RLM3041, Ruiying Biological, China), and Anti-PARP (1:1000, 9542, Cell Signaling Technology, USA).



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