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Dojindo Labs sodium cholate amount described dojindo
Sodium Cholate Amount Described Dojindo, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 92/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cholate/Sodium+cholate/us11208686-164-58-62
Average 92 stars, based on 19 article reviews

sodium cholate amount described dojindo - by Bioz Stars, 2026-11

92/100 stars

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Recombinant:

Article Title: Structural insights into the G protein selectivity revealed by the human EP3-G i signaling complex.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins FLAG peptide Scrum N/A EDTA-free complete inhibitor cocktail tablets Roche Cat# 05056489001 PGE2 Cayman Cat#14010 DDM (n-Dodecyl-b-D-Maltopyranoside), Sol-Grade Anatrace Cat#D310S LMNG (Lauryl Maltose Neopentyl Glycol) Anatrace Cat#NG310 GDN Anatrace Cat#GDN101 Sodium Cholate Dojindo CatC321 cholesterol hemi-succinate Sigma Cat#C6512 Cholesterol Sigma Cat#C8667 Iodoacetamide Wako Cat#093-02892 Ni-NTA Superflow Qiagen Cat#30450 Anti-FLAG M1 agarose affinity Gel Sigma Cat#4596 Sf9 expression medium WAKO Cat#160-25851 DMEM Nacalai tesque Cat#08456-65 PEI MAX Polyscience Cat#24765 D-luciferin Wako Cat#126-05116 PF-04418948 Cayman Cat#15016 Forskolin Wako Cat#067-02191 Deposited data EP3-Gi signaling complex This paper PDB ID: 7WU9 EMD-32824 EMPIAR-11119 Software and algorithms SerialEM Schorb et al. (2019) https://bio3d.colorado.edu/SerialEM Chimera N/A phenix.real_space_refine Adams et al. (2010) https://www.phenix-online.org/ Coot Emsley et al. (2010) https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ RELION-3.1 Zivanov et al. (2018) https://www3.mrc-lmb.cam.ac.uk/relion Gctf Zhang (2016) https://www2.mrc-lmb.cam.ac.uk/research/locally- developed-software/zhang-software/#gctf MotionCor2 Zheng et al. (2017) https://emcore.ucsf.edu/ucsf-software SIDESPLITTER Ramlaul et al. (2020) https://github.com/StructuralBiology-ICLMedicine/ SIDESPLITTER ..

Article Title: Crystal Structures of Human Orexin 2 Receptor Bound to the Subtype-Selective Antagonist EMPA.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, Peptides, and Recombinant Proteins FLAG peptide Scrum N/A EDTA-free complete inhibitor cocktail tablets Roche Cat# 05056489001 .. DDM (n-Dodecyl-b-D-Maltopyranoside), Sol-Grade Anatrace Cat#D310S L-MNG (Lauryl Maltose Neopentyl Glycol) Anatrace Cat#NG310 Sodium Cholate Dojindo CatC321 cholesterol hemi-succinate Sigma Cat#C6512 Monoolein(1-Oleoyl-rac-glycerol) Sigma Cat#M7765 Choresterol Sigma Cat#C8667 Iodoacetamide Wako Cat#093-02892 3H-EMPA GE helthcare N/A 3H-ZM241385 GE helthcare N/A Deposited Data OX2R-EMPA (SACLA) PDB, This Study 5WS3 OX2R-EMPA (Synchrotron) PDB, This Study 5WQC Experimental Models: Cell Lines Sf9 cells Invitrogen Cat#11496-015 Recombinant DNA OX2R-PGS GeneScript N/A Software and Algorithms PyMOL 1.8 Schrödinger http://www.pymol.org/ SHIKA Ueno et al., 2016 N/A KUMA Hirata et al., 2016 N/A KAMO N/A https://github.com/keitaroyam/yamtbx/blob/ master/doc/kamo-en.md BLEND Foadi et al., 2013 http://www.ccp4.ac.uk/ XDS Kabsch, 2010 http://xds.mpimf-heidelberg.mpg.de XSCALE Kabsch, 2010 http://xds.mpimf-heidelberg.mpg.de/html_ doc/xscale_program.html Cheetah Barty et al., 2014 http://www.desy.de/ barty/cheetah/Cheetah/ CrystFEL 0.6.2 White et al., 2012, 2016 http://www.desy.de/ twhite/crystfel/ Dirax Duisenberg, 1992 http://www.crystal.chem.uu.nl/distr/dirax/ Molrep Vagin and Teplyakov, 2000 http://www.ccp4.ac.uk/ phenix.refine Adams et al., 2010 https://www.phenix-online.org/ Coot Emsley et al., 2010 https://www2.mrc-lmb.cam.ac.uk/personal/ pemsley/coot/ PROMALS3D web server Pei and Grishin, 2007 http://prodata.swmed.edu/promals3d/ promals3d.php CHARMM-GUI Jo et al., 2008 www.charm-gui.org RESP Bayly et al., 1993 http://ambermd.org/ GAUSSIAN 09 software package Frisch, 2009 http://gaussian.com/glossary/g09/ GAFF Wang et al., 2004 http://ambermd.org/ ANTECHAMBER module Wang et al., 2006 http://ambermd.org/ (Continued on next page) e1 Structure 26, 1–13.e1–e5, January 2, 2018 .. REAGENT or RESOURCE SOURCE IDENTIFIER AMBER FF14SB Maier et al., 2015 http://ambermd.org/ LIPID14 Dickson et al., 2014 http://ambermd.org/ The modified PMEMD.CUDA program Case et al., 2014 http://ambermd.org/ Desmond version 2.3 Bowers et al., 2006 https://www.deshawresearch.com/ Maestro Schrödinger https://www.schrodinger.com/maestro Delphi package Liu et al., 2012 http://compbio.clemson.edu/delphi Other Ni-NTA Superflow Qiagen Cat#30450 Anti-FLAG M1 agarose affinity Gel Sigma Cat#4596 Sf9 expression mediam Expression Systems Cat#96-001-01 Whatman GF/B filters BRANDEL Cat#FPD136

Expressing:

Software:

Clear Native PAGE:

Article Title: TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria.
Article Snippet: .. Clear-native PAGE was performed as described previously.30 For the native antibody-based mobility shift (NAMOS) assay, the digitonin-solubilized mitochondrial proteins were incubated at 4 Cwith the indicated antibody, and then subjected to electrophoresis on 4%–16% gradient gels (Thermo Fisher) in the running buffer (50 mM tricine [pH 7.0] and 7.5 mM imidazole) containing 0.05% sodium cholate (Dojindo Laboratories) and 0.01% n-heptyl-b-D-thioglucoside (Dojindo Laboratories). ..

Mobility Shift:

Incubation:

Electrophoresis:

Mutagenesis:

Article Title: Spatially restricted substrate-binding site of cortisol-synthesizing CYP11B1 limits multiple hydroxylations and hinders aldosterone synthesis.
Article Snippet: Purification by column chromatography was carried out using Ni-NTA Superflow (Qiagen), DEAE Sepharose FF, and SP Sepharose FF (GE Healthcare). .. CYP11B1 proteins were eluted from SP Sepharose columns with a buffer consisting of 40 mM potassium phosphate (pH 7.4), 20% glycerol, 10 mM imidazole (Nacalai Tesque), 1% sodium cholate (Dojindo Molecular Technologies), 0.1% Tween 20, 0.1 mM PMSF, 0.1 mM Na EDTA, 0.1 mM DTT, and 75 mM NaCl for the wild type or 120 mM NaCl for the mutant. .. The eluates were analyzed using SDS-polyacrylamide gel (Extra PAGE One Precast Gel 7.5–15%, Nacalai Tesque) with molecular weight markers (LMW marker kit, GE Healthcare), and proteins were stained with Coomassie Brilliant blue.

Article Title: Spatially restricted substrate-binding site of cortisol-synthesizing CYP11B1 limits multiple hydroxylations and hinders aldosterone synthesis
Article Snippet: Purification by column chromatography was carried out using Ni-NTA Superflow (Qiagen), DEAE Sepharose FF, and SP Sepharose FF (GE Healthcare). .. CYP11B1 proteins were eluted from SP Sepharose columns with a buffer consisting of 40 mM potassium phosphate (pH 7.4), 20% glycerol, 10 mM imidazole (Nacalai Tesque), 1% sodium cholate (Dojindo Molecular Technologies), 0.1% Tween 20, 0.1 mM PMSF, 0.1 mM Na EDTA, 0.1 mM DTT, and 75 mM NaCl for the wild type or 120 mM NaCl for the mutant. .. The eluates were analyzed using SDS-polyacrylamide gel (Extra PAGE One Precast Gel 7.5–15%, Nacalai Tesque) with molecular weight markers (LMW marker kit, GE Healthcare), and proteins were stained with Coomassie Brilliant blue.

Membrane:

Article Title: Monomeric structure of an active form of bovine cytochrome c oxidase
Article Snippet: .. Fifty milliliters of 40% (w/v) sodium cholate (Dojindo, highest grade) was added to the mitochondrial membrane fraction, and then ammonium sulfate was added to 33% saturation at 0 °C while stirring (30 min). ..

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