chip assays (Rockland Immunochemicals)

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Rockland Immunochemicals chip assays

FIGURE 8. In situ binding of RREB-1 or HDAC1 to the HLA-G pro- moter in a repressive and active-type chromatin. A and B, <t>ChIP</t> performed with JEG-3 (HLA-G ) and M8 (HLA-G ) cells using anti-RREB-1 and anti-HDAC1 Abs on distal and proximal promoter regions (A) Abs target- ing RNA polymerase II <t>(RNApolII),</t> <t>acetylated</t> histone H3 (AcH3), and phosphorylated histone H3 (AcH3 P) on proximal promoter region (B). Immunoprecipitated HLA-G promoter regions are analyzed on agarose gels by semiquantitative HLA-G-speciﬁc PCRs targeting proximal and distal HLA-G promoter. Input chromatin (Input) used as PCR control and IgG () are shown. The absence of RREB-1 and HDAC1 binding observed in JEG-3 cells and the absence of RNA polymerase II, acetylated histone H3, and phosphorylated histone H3 binding in M8 cells validate the speciﬁcity of Abs used in ChIP assays.

Chip Assays, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-seq+kit/Transcription+Factor+Targeted+ChIP-seq+Kit/pm19890057-127-0-11
Average 92 stars, based on 4 article reviews

chip assays - by Bioz Stars, 2026-10

92/100 stars

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1) Product Images from "RREB-1 is a transcriptional repressor of HLA-G."

Article Title: RREB-1 is a transcriptional repressor of HLA-G.

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi: 10.4049/jimmunol.0902053

FIGURE 8. In situ binding of RREB-1 or HDAC1 to the HLA-G pro- moter in a repressive and active-type chromatin. A and B, ChIP performed with JEG-3 (HLA-G ) and M8 (HLA-G ) cells using anti-RREB-1 and anti-HDAC1 Abs on distal and proximal promoter regions (A) Abs target- ing RNA polymerase II (RNApolII), acetylated histone H3 (AcH3), and phosphorylated histone H3 (AcH3 P) on proximal promoter region (B). Immunoprecipitated HLA-G promoter regions are analyzed on agarose gels by semiquantitative HLA-G-speciﬁc PCRs targeting proximal and distal HLA-G promoter. Input chromatin (Input) used as PCR control and IgG () are shown. The absence of RREB-1 and HDAC1 binding observed in JEG-3 cells and the absence of RNA polymerase II, acetylated histone H3, and phosphorylated histone H3 binding in M8 cells validate the speciﬁcity of Abs used in ChIP assays.

Figure Legend Snippet: FIGURE 8. In situ binding of RREB-1 or HDAC1 to the HLA-G pro- moter in a repressive and active-type chromatin. A and B, ChIP performed with JEG-3 (HLA-G ) and M8 (HLA-G ) cells using anti-RREB-1 and anti-HDAC1 Abs on distal and proximal promoter regions (A) Abs target- ing RNA polymerase II (RNApolII), acetylated histone H3 (AcH3), and phosphorylated histone H3 (AcH3 P) on proximal promoter region (B). Immunoprecipitated HLA-G promoter regions are analyzed on agarose gels by semiquantitative HLA-G-speciﬁc PCRs targeting proximal and distal HLA-G promoter. Input chromatin (Input) used as PCR control and IgG () are shown. The absence of RREB-1 and HDAC1 binding observed in JEG-3 cells and the absence of RNA polymerase II, acetylated histone H3, and phosphorylated histone H3 binding in M8 cells validate the speciﬁcity of Abs used in ChIP assays.

Techniques Used: In Situ, Binding Assay, Immunoprecipitation, Control

Activity Assay:

Article Title: Established and novel NF-κB inhibitors lead to downregulation of TLR3 and the proliferation and cytokine secretion in HNSCC.
Article Snippet: 1368-8375/$ see front matter 2011 Elsevier Ltd. A doi:10.1016/j.oraloncology.2011.06.010 ⇑ Corresponding author.. Address: UK-SH, Klinik für 23538 Lübeck, Germany.. Tel.

Article Title: Deletion of angiotensin-converting enzyme 2 promotes the development of atherosclerosis and arterial neointima formation.
Article Snippet: . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Deletion of angiotensin-converting enzyme 2 promotes the development of atherosclerosis and arterial neointima formation

Transcription Factor Assay:

Knockdown:

Chromatin Immunoprecipitation:

Article Title: RREB-1 is a transcriptional repressor of HLA-G.
Article Snippet: .. ChIP assays were performed as previously described (51) using antiRREB-1 from Rockland; anti-acetylated histone H3 (06-599) and antiphosphorylated Ser10 histone H3 (07-081) from Upstate Biotechnology Associates; and anti-RNApolII (C-21) and anti-HDAC1 (H-51) from Santa Cruz Biotechnology. ..

Article Title: Metformin Treatment Leads to Increased HIV Transcription and Gene Expression through Increased CREB Phosphorylation and Recruitment to the HIV LTR Promoter
Article Snippet: The protein concentration of the cleared cell lysates was also determined using a Bio-Rad DC protein assay kit (BioRad) and used to normalize the luciferase activity, which was expressed as relative luminescence/light unit (rlu). .. The ChIP assay was performed according to the protocol established by Rockland Immunochemicals, Inc. (Pottstown, PA) with some modifications. .. Briefly, cells were washed with ice-cold PBS twice, added freshly made 5 mM dimethyl 3,3′-dithio-bis (propionimidate) dihydrochloride (#38285-78-8, Sigma-Aldrich), incubated on ice for 30 min, added ice-cold quenching buffer (100 mM Tris.HCl pH 8.0, 150 mM NaCl), and then washed with ice-cold PBS twice.

Review