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Gallus BioPharmaceuticals h3 trimethylation chip-seq analysis
<t>H3</t> <t>trimethylation</t> loci. The total number of peaks significantly identified were categorized according to their regional chromosomes (A). Each color bar presented in the legend was described as following: , peaks shared among all samples with either K4 or K27 trimethylation (All_shared_K4_and_K27); , peaks presented only among samples with K4 trimethylation (All_shared_K4_but_not_K27); , peaks presented only among samples with K27 trimethylation (All_shared_K27_but_not_K4); , peaks presented only in Lat_K4 (Unique_Lat_K4); , peaks presented only in Med_K4 (Unique_Med_K4); , peaks presented only in PS_K4 (Unique_PS_K4); , peaks presented only in RS_K4 (Unique_RS_K4); , peaks presented only in PS_K27 (Unique_PS_K27); , peaks presented only in RS_K27 (Unique_RS_K27), and , other peaks not in the previously mentioned groups. Distribution of precipitated DNA fragments acquired from sample ChIP-seq data according to chicken gene transcription start site was also demonstrated (B). Aggregation of fragments within 0–1 kilobases ( ) and 1–3 kilobases ( ) are presented.
H3 Trimethylation Chip Seq Analysis, supplied by Gallus BioPharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-seq+analysis/pmc05933975-122-9-16?v=Gallus+BioPharmaceuticals
Average 90 stars, based on 1 article reviews
h3 trimethylation chip-seq analysis - by Bioz Stars, 2026-08
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1) Product Images from "A demonstration of the H3 trimethylation ChIP-seq analysis of galline follicular mesenchymal cells and male germ cells"

Article Title: A demonstration of the H3 trimethylation ChIP-seq analysis of galline follicular mesenchymal cells and male germ cells

Journal: Asian-Australasian Journal of Animal Sciences

doi: 10.5713/ajas.17.0744

H3 trimethylation loci. The total number of peaks significantly identified were categorized according to their regional chromosomes (A). Each color bar presented in the legend was described as following: , peaks shared among all samples with either K4 or K27 trimethylation (All_shared_K4_and_K27); , peaks presented only among samples with K4 trimethylation (All_shared_K4_but_not_K27); , peaks presented only among samples with K27 trimethylation (All_shared_K27_but_not_K4); , peaks presented only in Lat_K4 (Unique_Lat_K4); , peaks presented only in Med_K4 (Unique_Med_K4); , peaks presented only in PS_K4 (Unique_PS_K4); , peaks presented only in RS_K4 (Unique_RS_K4); , peaks presented only in PS_K27 (Unique_PS_K27); , peaks presented only in RS_K27 (Unique_RS_K27), and , other peaks not in the previously mentioned groups. Distribution of precipitated DNA fragments acquired from sample ChIP-seq data according to chicken gene transcription start site was also demonstrated (B). Aggregation of fragments within 0–1 kilobases ( ) and 1–3 kilobases ( ) are presented.
Figure Legend Snippet: H3 trimethylation loci. The total number of peaks significantly identified were categorized according to their regional chromosomes (A). Each color bar presented in the legend was described as following: , peaks shared among all samples with either K4 or K27 trimethylation (All_shared_K4_and_K27); , peaks presented only among samples with K4 trimethylation (All_shared_K4_but_not_K27); , peaks presented only among samples with K27 trimethylation (All_shared_K27_but_not_K4); , peaks presented only in Lat_K4 (Unique_Lat_K4); , peaks presented only in Med_K4 (Unique_Med_K4); , peaks presented only in PS_K4 (Unique_PS_K4); , peaks presented only in RS_K4 (Unique_RS_K4); , peaks presented only in PS_K27 (Unique_PS_K27); , peaks presented only in RS_K27 (Unique_RS_K27), and , other peaks not in the previously mentioned groups. Distribution of precipitated DNA fragments acquired from sample ChIP-seq data according to chicken gene transcription start site was also demonstrated (B). Aggregation of fragments within 0–1 kilobases ( ) and 1–3 kilobases ( ) are presented.

Techniques Used: ChIP-sequencing

Chromatin histone 3 (H3) trimethylation state and transcription of cellular retinoic acid binding protein 1 ( CRABP1 ), growth differentiation factor 10 ( GDF10 ), and gremlin 1 ( GREM1 ) genes. The heatmaps of CRABP1, GDF10 , and GREM1 genes were acquired from the integrative genomics viewer (IGV) interface. The chicken full genome sequences was used to identify the chromatin loci of these genes with transcription direction indicated by the arrow heads presented in the genome along with the associated promoters in the area (galGal5 and Promoter regions at the bottom of the figure). The figure show 3 types of data—ChIP-seq, RNA-seq, and Peak files acquired from each sample cell type. In brief, ChIP-seq data show the density of DNA fragments aligned to a chromatin region (aggregated DNA fragments) in blue heatmap. The RNA-seq data show the density of transcript fragments aligned to the gene regions representing level of gene expressions by red heatmap. Finally, the peak file of each cell sample indicated the chromatin region with significant peaks identifed by peak calling analysis.
Figure Legend Snippet: Chromatin histone 3 (H3) trimethylation state and transcription of cellular retinoic acid binding protein 1 ( CRABP1 ), growth differentiation factor 10 ( GDF10 ), and gremlin 1 ( GREM1 ) genes. The heatmaps of CRABP1, GDF10 , and GREM1 genes were acquired from the integrative genomics viewer (IGV) interface. The chicken full genome sequences was used to identify the chromatin loci of these genes with transcription direction indicated by the arrow heads presented in the genome along with the associated promoters in the area (galGal5 and Promoter regions at the bottom of the figure). The figure show 3 types of data—ChIP-seq, RNA-seq, and Peak files acquired from each sample cell type. In brief, ChIP-seq data show the density of DNA fragments aligned to a chromatin region (aggregated DNA fragments) in blue heatmap. The RNA-seq data show the density of transcript fragments aligned to the gene regions representing level of gene expressions by red heatmap. Finally, the peak file of each cell sample indicated the chromatin region with significant peaks identifed by peak calling analysis.

Techniques Used: Binding Assay, ChIP-sequencing, RNA Sequencing

UpSet plot of intersected peaks among cell types. The intersected peaks shared among cell types (Lat_K4, Med_K4, PS_K4, RS_K4, PS_K27, and RS_K27) were presented by UpSet plot. The connected lines among cell types shown in the lower panel of the plot represented the group of intersected peaks among them. The upper panel bar graph of the plot presented the number of peaks found in each group. The shared peak number among all samples with either K4 or K27 trimethylation and only K4 trimethylation are in red ( ) and orange ( ) color, accordingly. The horizontal leftmost bar graph (olive green color, ) show to total number of peaks presented in each cell type.
Figure Legend Snippet: UpSet plot of intersected peaks among cell types. The intersected peaks shared among cell types (Lat_K4, Med_K4, PS_K4, RS_K4, PS_K27, and RS_K27) were presented by UpSet plot. The connected lines among cell types shown in the lower panel of the plot represented the group of intersected peaks among them. The upper panel bar graph of the plot presented the number of peaks found in each group. The shared peak number among all samples with either K4 or K27 trimethylation and only K4 trimethylation are in red ( ) and orange ( ) color, accordingly. The horizontal leftmost bar graph (olive green color, ) show to total number of peaks presented in each cell type.

Techniques Used:



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