chip seq analysis (Arraystar inc)
90
Structured Review
Arraystar inc
chip seq analysis
Chip Seq Analysis, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-seq+analysis/chip+seq+analysis/pmc08997160-54-8-11
Average 90 stars, based on 1 article reviews
Chip Seq Analysis, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip-seq+analysis/chip+seq+analysis/pmc08997160-54-8-11
Average 90 stars, based on 1 article reviews
chip seq analysis - by Bioz Stars,
2026-09
90/100 stars
Images
1) Product Images from "SMYD3 Promotes Cell Cycle Progression by Inducing Cyclin D3 Transcription and Stabilizing the Cyclin D1 Protein in Medulloblastoma"
Article Title: SMYD3 Promotes Cell Cycle Progression by Inducing Cyclin D3 Transcription and Stabilizing the Cyclin D1 Protein in Medulloblastoma
Journal: Cancers
doi: 10.3390/cancers14071673
Figure Legend Snippet: Chromatin immunoprecipitation (ChIP) analysis of transcriptomic and genome-wide SMYD3 binding profiles in MB. ( A ) Heat map showing the distribution and peak intensity of SMYD3 genomic occupancy from 5 kb downstream to 5 kb upstream. ( B ) Schematic representation of peaks into promoter peaks, upstream peaks, intron peaks, exon peaks, and intergenic peaks; pie diagram showing the distribution of peaks. ( C ) Graph depicting the ChIP peak at the global TSS (green) vs. Input peak (orange). ( D ) Pathway analysis using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showing SMYD3 enrichment scores. ( E ) List of genes and their representative pathways whose promoters were bound by SMYD3, as predicted by KEGG pathway analysis.
Techniques Used: Chromatin Immunoprecipitation, Genome Wide, Binding Assay
Figure Legend Snippet: SMYD3 binds to the Cyclin D3 promoter. ( A ) Representation of the cyclin D3 (CCND3) gene promoter sequence; (red circles) the EPD software detected four SMYD3 consensus binding sites (−1166, −890, −247, −121 bps) on the cyclin D3 gene promoter. (Blue box) Region −311 to +367 was identified by SMYD3_ChIP-seq analysis. ( B ) Agarose gel showing the PCR amplification of RI (ChIP identified sequence), RII (SMYD3 binding sites within RI), and RIII regions (negative control). ( C ) Scheme depicting the RI, RII, and RIII regions cloned into the pXPG reporter vector. ( D ) Sequence peaks showing the SMYD3 binding sites on the cloned CCND3 promoter regions within the pXPG plasmid. ( E , F ) Effect of SMYD3 on Cyclin D3 (RI region) promoter activity by luciferase reporter assay in D458 (top) and MB002 (bottom) cells. D458 and MB002 cells were transfected with shRNA or overexpression vectors targeting SMYD3 for 48 h or treated with BCI-121 (80 μM) for 24 h. The statistical significance * p < 0.05, ** p < 0.01, *** p < 0.001.
Techniques Used: Sequencing, Software, Binding Assay, ChIP-sequencing, Agarose Gel Electrophoresis, Amplification, Negative Control, Clone Assay, Plasmid Preparation, Activity Assay, Luciferase, Reporter Assay, Transfection, shRNA, Over Expression
Related Articles
Chromatin Immunoprecipitation:Article Title: SMYD3 Promotes Cell Cycle Progression by Inducing Cyclin D3 Transcription and Stabilizing the Cyclin D1 Protein in Medulloblastoma Article Snippet: ChIP assay was performed with the ChIP-IT Express Kit (Active Motif, Carlsbad, CA, USA) using standard protocol [ ]. .. The anti-SMYD3 antibody ChIP-enriched DNA was sent for |