pre cooled chip immunoprecipitation buffer (Thermo Fisher)
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pre cooled chip immunoprecipitation buffer
Pre Cooled Chip Immunoprecipitation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip+buffer/HEPES/pmc13006433-397-27-64
Average 99 stars, based on 1 article reviews
Pre Cooled Chip Immunoprecipitation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip+buffer/HEPES/pmc13006433-397-27-64
Average 99 stars, based on 1 article reviews
pre cooled chip immunoprecipitation buffer - by Bioz Stars,
2026-10
99/100 stars
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Immunoprecipitation:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: Staining:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM Polymerase Chain Reaction:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: Western Blot:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: Recombinant:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: Software:Article Title: The basic domain of Suv39h2 buffers mitoxantrone-induced heterochromatin destabilization. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies goat polyclonal α-GFP Rockland Cat#: 600-141-215; RRID: AB_1961516 rabbit polyclonal α-GFP Invitrogen Cat#: A11122; RRID: AB_221569 mouse monoclonal α-GAPDH SantaCruz Biotechnology Cat#: sc-32233; RRID: AB_627679 rabbit monoclonal α-HP1α Abcam Cat#: ab109028; RRID: AB_10858495 rabbit polyclonal α-H3K9me3 Abcam Cat#: 8898; RRID: AB_306848 mouse polyclonal α-H2A.X (Ser139) Millipore Cat#: 05-636; RRID: AB_309864 rabbit monoclonal α-H3 Abcam Cat#: ab176842; RRID: AB_2493104 rabbit polyclonal α-H3K9me3 Jenuwein lab # 1926 mouse monoclonal α-Nucleophosmin Abcam Cat#: ab10530; RRID: AB_297271 Chemicals, peptides, and recombinant proteins High-glucose Dulbecco’s Modified Eagle Medium (DMEM) Sigma-Aldrich Cat#: D6171 HyClone Characterized Fetal bovine serum Cytiva Cat#: SH30071.03 L-glutamine Sigma-Aldrich Cat#: G7513 β-mercaptoethanol Gibco Cat#: 31350-010 MEM Non-essential Amino Acid Solution (100×) Sigma-Aldrich Cat#: M7145 sodium pyruvate solution Sigma-Aldrich Cat#: S8636 penicillin-streptomycin Sigma-Aldrich Cat#: P4333 puromycin dihydrochloride Sigma-Aldrich Cat#: P9620 XhoI restriction enzyme New England Biolabs Cat#: R0146S AflII restriction enzyme New England Biolabs Cat#: R05020S PvuI restriction enzyme New England Biolabs Cat#: R3150S XFect Transfection reagent Takara Cat#: 631317 DPBS Gibco Cat#: 14190-094 RIPA Pierce Cat#: 89900 cOmplete EDTA-free protease inhibitors Roche Cat#: 11873580001 paraformaldehyde Santa Cruz Cat#: sc-281692 triton X-100 Sigma-Aldrich Cat#: X100 VECTASHIELD Antifade Mounting Medium with DAPI Vector Leboratories Cat#: H-1200 donkey serum Jackson ImmunoResearch Cat#: 017-000-121 phenol red-free DMEM Gibco Cat#: 21063029 1,6-hexanediol Sigma-Aldrich Cat#: 240117 low melting point agarose Bethesda Scientific Cat#: V3841 RNaseA Thermo Scientific Cat#: EN0531 mitoxantrone Selleckchem Cat#: S2485 cbl-0137 Selleckchem Cat#: S0507 formaldehyde Sigma-Aldrich Cat#: F8775 glycine Sigma-Aldrich Cat#: G8898 SDS Sigma-Aldrich Cat#: L3771 EDTA Sigma-Aldrich Cat#: 798681 tris-HCl Sigma-Aldrich Cat#: 108315 (Continued on next page) iScience 29, 115626, May 15, 2026 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER NaCl Sigma-Aldrich Cat#: S5886 dynabeads Protein G for Immunoprecipitation Invitrogen Cat#: 10003D LiCl Merck Cat#: 310468 sodium deoxycholate Sigma-Aldrich Cat#: D6750 NaHCO3 Merck Cat#: S6297 proteinase K Thermo Scientific Cat#: EO0491 MNase Thermo Scientific Cat#: EN0181 SYBR Select Master Mix Applied Biosystems Cat#: 4472908 SYBR Safe DNA Gel Stain Invitrogen Cat#: other:Article Title: Establishment of a broiler jejunal organoid model based on optimized activators or optimized two-step media and its assessment Article Snippet: Media compositions were as follows: CMDM: 50% ( v / v ) CM, 37% ( Isolation:Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM Cell Culture:Article Title: T-tubular remodeling occurs in a cardiomyocyte-autonomous manner following pathological mechanical stiffness exposure Article Snippet: .. Isolated cardiomyocytes were plated onto 10 μg/ml laminin-coated surfaces and maintained in M199 (ThermoFisher Scientific) supplemented with 1× insulin-transferrinselenium-X (Sigma), 1 μg/μl primocin (InvivoGen), and 20 mM Article Title: The influence of simulated microgravity on MG-63 osteoblast-like cells cultured on polymeric scaffold Article Snippet: .. All cell culture media and supplements were purchased from PAN-Biotech, except for non-essential amino acids (NEAA), sodium pyruvate, and (4-( |