Review



chromatin extraction buffer (1 m m edta, 0.5 m m egta, 10 m m tris-hcl, ph 8.1)  (DIAGENODE DIAGNOSTICS)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    DIAGENODE DIAGNOSTICS chromatin extraction buffer (1 m m edta, 0.5 m m egta, 10 m m tris-hcl, ph 8.1)
    Chromatin Extraction Buffer (1 M M Edta, 0.5 M M Egta, 10 M M Tris Hcl, Ph 8.1), supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/np+chip+buffer++150+mm+nacl++5+mm+edta++0+5++igepal+ca+630++1++triton+x+100+in+50+mm+tris+hcl+ph+7+5+/pmc03537039-122-8-37
    Average 90 stars, based on 1 article reviews
    chromatin extraction buffer (1 m m edta, 0.5 m m egta, 10 m m tris-hcl, ph 8.1) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    other:

    Article Title: The uncharacterized SANT and BTB domain-containing protein SANBR inhibits class switch recombination
    Article Snippet: Cells were isolated by centrifugation at 135 g for 6 min and sonicated in IP buffer (30 mM Tris pH 8, 100 mM NaCl, 5 mM EDTA, 0.02% NaN3, 0.3% SDS, 1.7% TritonX-100) using Bioruptor Pico (Diagenode) for ten cycles (30 s on, 30 s off).

    Article Title: Dynamic control of gene expression by ISGF3 and IRF1 during IFNβ and IFNγ signaling.
    Article Snippet: Pellets were mixed with 3ml of LB3 (10mM Tris, 100mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% deoxycholate and 0.5% N-lauroylsarcosine) and split into two in 15-ml polypropylene tubes suitable for the Bioruptor® Pico (Diagenode, Catalog # C01020031).

    Article Title: A proteomics study identifying interactors of the FSHD2 gene product SMCHD1 reveals RUVBL1-dependent DUX4 repression.
    Article Snippet: Cells were collected and lysed in NP-ChIP-buffer (150 mM NaCl, 5 mm EDTA, 0.5% Igepal CA-630, 1% Triton X-100 in 50 mM Tris–HCl pH 7.5) to isolate cell nuclei, which were sheared using a Bioruptor Pico (Diagenode) (Myotubes: 25 cycles, 30 s on/ off in 1.5 ml Bioruptor Pico sonication tubes (Diagenode)).

    Article Title: CTCF confers local nucleosome resiliency after DNA replication and during mitosis
    Article Snippet: Mitotic and interphase cells were then treated in parallel to sonicate the chromatin in 300 μl of TSE150 (0.1% SDS, 1% Triton X-100, 2 mM EDTA, 20 mM Tris-HCl pH8, 150 mM NaCl; freshly supplemented with 1 × PIC) using 1.5 ml tubes (Diagenode) and a Bioruptor Pico (Diagenode; seven cycles divided into 30 s ON–30 s OFF sub-cycles at maximum power, in circulating ice-cold water).

    Article Title: Formin 2 links neuropsychiatric phenotypes at young age to an increased risk for dementia
    Article Snippet: In brief, tissue was lysed in RIPA-SDS (140mM NaCl, 1mM EDTA, 1% Triton-X-100, 0.1% sodium deoxycholate, 10mM Tris-HCl, pH8, 1%SDS) buffer for 10min at 4C and then sonicated for 20min in a Bioruptor plus NGS (Diagenode).

    Extraction:

    Article Title: PA1 Protein, a New Competitive Decelerator Acting at More than One Step to Impede Glucocorticoid Receptor-mediated Transactivation
    Article Snippet: .. The pellets were resuspended in 300 μl of chromatin extraction buffer (1 m m EDTA, 0.5 m m EGTA, 10 m m Tris-HCl, pH 8.1) per dish (0 °C for ≥10 min) and sonicated with a BIORUPTOR (DIAGENODE; 6 cycles of 30 s on and 30 s off for 5 min at high power immersed in ice-water bath). .. After centrifugation (15,000 × g for 10 min at 4 °C), the supernatant was diluted into 5.4 ml of chromatin extraction buffer with 1% Triton X-100 and 0.1% sodium deoxycholate and treated with 200 μl of preblocked protein A beads (GE Healthcare) with gentle mixing (4 °C for 1 h on a rotating drum at 4 rpm).

    Sonication:

    Article Title: PA1 Protein, a New Competitive Decelerator Acting at More than One Step to Impede Glucocorticoid Receptor-mediated Transactivation
    Article Snippet: .. The pellets were resuspended in 300 μl of chromatin extraction buffer (1 m m EDTA, 0.5 m m EGTA, 10 m m Tris-HCl, pH 8.1) per dish (0 °C for ≥10 min) and sonicated with a BIORUPTOR (DIAGENODE; 6 cycles of 30 s on and 30 s off for 5 min at high power immersed in ice-water bath). .. After centrifugation (15,000 × g for 10 min at 4 °C), the supernatant was diluted into 5.4 ml of chromatin extraction buffer with 1% Triton X-100 and 0.1% sodium deoxycholate and treated with 200 μl of preblocked protein A beads (GE Healthcare) with gentle mixing (4 °C for 1 h on a rotating drum at 4 rpm).



    Similar Products

    99
    Thermo Fisher chip sonication buffer
    Chip Sonication Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/HEPES/pmc12993448-105-5-46
    Average 99 stars, based on 1 article reviews
    chip sonication buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc 1x chip buffer
    1x Chip Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/pmc13019507-442-12-15
    Average 86 stars, based on 1 article reviews
    1x chip buffer - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc chip lysis buffer
    Chip Lysis Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/pmc13043960-89-4-7
    Average 86 stars, based on 1 article reviews
    chip lysis buffer - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    99
    Thermo Fisher pre cooled chip immunoprecipitation buffer
    Pre Cooled Chip Immunoprecipitation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/HEPES/pmc13006433-397-27-64
    Average 99 stars, based on 1 article reviews
    pre cooled chip immunoprecipitation buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology pre cooled chip immunoprecipitation buffer
    Pre Cooled Chip Immunoprecipitation Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/Triton+X-100/pmc13006433-397-27-76
    Average 96 stars, based on 1 article reviews
    pre cooled chip immunoprecipitation buffer - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    99
    Thermo Fisher chip immunoprecipitation buffer
    Chip Immunoprecipitation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/Protease+Inhibitor+Cocktail/pmc13006433-387-5-42
    Average 99 stars, based on 1 article reviews
    chip immunoprecipitation buffer - by Bioz Stars, 2026-09
    99/100 stars
      Buy from Supplier

    86
    Cell Signaling Technology Inc chip elution buffer
    Chip Elution Buffer, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chip+buffer/pm41839241-105-4-7
    Average 86 stars, based on 1 article reviews
    chip elution buffer - by Bioz Stars, 2026-09
    86/100 stars
      Buy from Supplier

    Image Search Results