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DIAGENODE DIAGNOSTICS chip buffer
Chip Buffer, supplied by DIAGENODE DIAGNOSTICS, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/chip+buffer/chip+buffer/bio_rxiv__192567-126-22-24
Average 90 stars, based on 1 article reviews
chip buffer - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

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Chromatin Immunoprecipitation:

Article Title: Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcriptional control
Article Snippet: .. The eluates were diluted 1:50 with ChIP buffer from the OneDay ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the OneDay ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C. ..

Article Title: SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4 repeat and LRIF1 promoter yet display different modes of action
Article Snippet: .. Final nuclear pellets were resuspended in the ChIP buffer and sonicated at the highest power output for 25 cycles (1 cycle: 30 sec ON/30 sec OFF) using a Bioruptor instrument (Diagenode). .. For ChIP, chromatin was first pre-cleared with BSA-blocked protein A Sepharose beads (GE Healthcare, #17-5280-21) by rotating for 30–60 min at 4 °C.

Article Title: ICP22-defined condensates mediate RNAPII deubiquitylation by UL36 and promote HSV-1 transcription.
Article Snippet: .. The precipitate was suspended by ChIP buffer and sonicated by Bioruptor Pico (Diagenode SA, Belgium). ..

Article Title: Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery
Article Snippet: .. Finally, pellets were resuspended with 100 μl of 1 × ChIP Buffer and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for five cycles with 30 s ON and 30 s OFF. ..

Article Title: A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Article Snippet: .. Next, the nuclei were pelleted at 15,500 g, 4 °C for 1 min, resuspended in 100 μl of ChIP buffer 1 and kept on ice for 10 min, followed by sonication (3 × 30 s on/off, high output) using Bioruptor sonicator (Diagenode). .. The lysate was cleared by centrifugation at 9200 g , 4 °C for 10 min and transferred to a fresh DNA LoBind 1.5 ml tube (Eppendorf).

Article Title: SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression.
Article Snippet: .. Nuclear pellet was finally resuspended in 100 μL of 1× ChIP Buffer, incubate on ice for 10 min, and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles for 30 s with 30 s between cycles. .. Supernatant was collected and incubated with antibodies (RELA (8242S, Cell Signaling Technology, 1 : 100), H3K4me1 (ab8895, Abcam, 2 μg for 25 μg of chromatin), and H3K27ac (ab177178, Abcam, 2 μg for 25 μg of chromatin)) bound to Magna ChIPTM Protein A+G Magnetic Beads (16-663, Millipore) at 4 °C overnight.

Article Title: Chromatin profiling reveals TFAP4 as a critical transcriptional regulator of bovine satellite cell differentiation
Article Snippet: .. Cell nuclei were sheared in ice-cold ChIP buffer from Diagenode (Denville, NJ, USA) to generate chromatin fragments of 200–1000 bp using a Model 100 sonic dismembrator. ..

Incubation:

Article Title: Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcriptional control
Article Snippet: .. The eluates were diluted 1:50 with ChIP buffer from the OneDay ChIP kit (Diagenode) and subsequently subjected to a second ChIP in accordance with the OneDay ChIP kit manual but performing an overnight antibody incubation under rotation at 4°C. ..

Sonication:

Article Title: SMCHD1 and LRIF1 converge at the FSHD-associated D4Z4 repeat and LRIF1 promoter yet display different modes of action
Article Snippet: .. Final nuclear pellets were resuspended in the ChIP buffer and sonicated at the highest power output for 25 cycles (1 cycle: 30 sec ON/30 sec OFF) using a Bioruptor instrument (Diagenode). .. For ChIP, chromatin was first pre-cleared with BSA-blocked protein A Sepharose beads (GE Healthcare, #17-5280-21) by rotating for 30–60 min at 4 °C.

Article Title: ICP22-defined condensates mediate RNAPII deubiquitylation by UL36 and promote HSV-1 transcription.
Article Snippet: .. The precipitate was suspended by ChIP buffer and sonicated by Bioruptor Pico (Diagenode SA, Belgium). ..

Article Title: Lupus enhancer risk variant causes dysregulation of IRF8 through cooperative lncRNA and DNA methylation machinery
Article Snippet: .. Finally, pellets were resuspended with 100 μl of 1 × ChIP Buffer and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for five cycles with 30 s ON and 30 s OFF. ..

Article Title: A critical role of PRDM14 in human primordial germ cell fate revealed by inducible degrons
Article Snippet: .. Next, the nuclei were pelleted at 15,500 g, 4 °C for 1 min, resuspended in 100 μl of ChIP buffer 1 and kept on ice for 10 min, followed by sonication (3 × 30 s on/off, high output) using Bioruptor sonicator (Diagenode). .. The lysate was cleared by centrifugation at 9200 g , 4 °C for 10 min and transferred to a fresh DNA LoBind 1.5 ml tube (Eppendorf).

Article Title: SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression.
Article Snippet: .. Nuclear pellet was finally resuspended in 100 μL of 1× ChIP Buffer, incubate on ice for 10 min, and subsequently sonicated at 4 °C with a Bioruptor sonicator (Diagenode) at high power for 5 cycles for 30 s with 30 s between cycles. .. Supernatant was collected and incubated with antibodies (RELA (8242S, Cell Signaling Technology, 1 : 100), H3K4me1 (ab8895, Abcam, 2 μg for 25 μg of chromatin), and H3K27ac (ab177178, Abcam, 2 μg for 25 μg of chromatin)) bound to Magna ChIPTM Protein A+G Magnetic Beads (16-663, Millipore) at 4 °C overnight.



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