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Applichem inc ches buffer
Ches Buffer, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ches+buffer/ches/pmc09867336-73-50-53
Average 90 stars, based on 1 article reviews
ches buffer - by Bioz Stars, 2026-11
90/100 stars

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Article Title: Application of Empirical Phase Diagrams for Multidimensional Data Visualization of High-Throughput Microbatch Crystallization Experiments.
Article Snippet: Accepted Manuscript Application Of Empirical Phase Diagrams For Multidimensional Data Visualization Of High Throughput Microbatch Crystallization Experiments Marieke E. Klijn, Jürgen Hubbuch PII: S0022-3549(18)30221-1 DOI: 10.1016/j.xphs.2018.04.018 Reference: XPHS 1143 To appear in: Journal of Pharmaceutical Sciences Received Date: 15 March 2018 Accepted Date: 20 April 2018 Please cite this article as: Klijn ME, Hubbuch J, Application Of Empirical Phase Diagrams For Multidimensional Data Visualization Of High Throughput Microbatch Crystallization Experiments, Journal of Pharmaceutical Sciences (2018), doi: 10.1016/j.xphs.2018.04.018.. This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

Article Title: Design of antibody variable fragments with reduced reactivity to preexisting anti-drug antibodies
Article Snippet: Composition of the mobile phase has previously been reported and consist of 15.6 mM CAPS (Sigma-Aldrich, #C2632), 9.4 mM CHES (AppliChem, #A1065), 4.6 mM TAPS (Sigma-Aldrich, #M8389), 9.9 mM HEPPSO (Molekula, #44974537), 8.7 mM MOPSO (Sigma-Aldrich, #M8389), 11 mM MES (AppliChem, #A0689), 13 mM acetic acid (AppliChem, #361008), and 9.9 mM formic acid (Honeywell, #09676). pH was adjusted to 4 and 11 using NaOH.

Article Title: Light Modulation for Bioactive Pigment Production in Synechocystis salina
Article Snippet: The growth of S. salina strain LEGE 06155 was performed in 2 L-round flasks (working volume of 1.8 L) in BG 11 culture medium [ ], supplemented with 10 g·L −1 of NaCl, with pH 8.9 ± 0.1 previously adjusted with CHES buffer (PanReach, AppliChem, Chicago, IL, USA), with temperature maintained at 23 °C.

Article Title: Light Modulation for Bioactive Pigment Production in Synechocystis salina .
Article Snippet: The growth of S. salina strain LEGE 06155 was performed in 2 L-round flasks (working volume of 1.8 L) in BG11 culture medium [27], supplemented with 10 g·L−1 of NaCl, with pH 8.9± 0.1 previously adjusted with CHES buffer (PanReach, AppliChem, Chicago, IL, USA), with temperature maintained at 23 ◦C.

Article Title: Design of antibody variable fragments with reduced reactivity to preexisting anti-drug antibodies.
Article Snippet: Composition of the mobile phase has previously been reported and consist of 15.6 mM CAPS (Sigma-Aldrich, #C2632), 9.4 mM CHES (AppliChem, #A1065), 4.6 mM TAPS (Sigma-Aldrich, #M8389), 9.9 mM HEPPSO (Molekula, #44974537), 8.7 mM MOPSO (SigmaAldrich, #M8389), 11 mM MES (AppliChem, #A0689), 13 mM acetic acid (AppliChem, #361008), and 9.9 mM formic acid (Honeywell, #09676). pH was adjusted to 4 and 11 using NaOH.30 Species eluting prior to or after the main peak were grouped as acidic and basic variants, respectively.

Article Title: Modeling of bispecific antibody elution in mixed-mode cation-exchange chromatography.
Article Snippet: The increasing demand for cost-efficient manufacturing of biopharmaceuticals has been the main driving force for the development of novel chromatography resins, which resulted in the development of multimodal or mixed-mode chromatographic resins.. Most of them combine electrostatic and hydrophobic functionalities and are designed to deliver unique selectivity and increased binding capacities also at increased ionic strength.. However, the mechanism of the protein–resin interaction in mixed-mode chromatography is still not fully understood.

Article Title: Correlating multidimensional short-term empirical protein properties to long-term protein physical stability data via empirical phase diagrams.
Article Snippet: Accepted Manuscript Correlating Multidimensional Short-Term Empirical Protein Properties to Long-Term Protein Physical Stability Data Via Empirical Phase Diagrams Marieke E. Klijn, Jürgen Hubbuch PII: S0378-5173(19)30129-2 DOI: https://doi.org/10.1016/j.ijpharm.2019.02.006 Reference: IJP 18144 To appear in: International Journal of Pharmaceutics Received Date: 22 November 2018 Revised Date: 31 January 2019 Accepted Date: 1 February 2019 Please cite this article as: M.E.. Klijn, J. Hubbuch, Correlating Multidimensional Short-Term Empirical Protein Properties to Long-Term Protein Physical Stability Data Via Empirical Phase Diagrams, International Journal of Pharmaceutics (2019), doi: https://doi.org/10.1016/j.ijpharm.2019.02.006 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.

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Article Title: Effects of Temperature, pH, and NaCl Concentration on Biomass and Bioactive Compound Production by Synechocystis salina
Article Snippet: Each run was established with an initial optical density (OD) of 0.1 (OD = λ 680 nm − λ 750 nm ), and the experiments were carried out in two L-flat bottom round flasks (1.8 L of working volume) maintained in batch over 22 d. The light was provided by fluorescent lamps (BIOLUX, OSRAM) with an intensity of 150 μmol photons ·m −2 ·s −1 and an L/D cycle of 16 h/8 h. Agitation was provided via continuous air bubbling, with an airflow of 0.75 L air ·L −1 ·min −1 . .. To maintain pH in the medium, different buffer solutions were added to a final concentration of 30 mM MES buffer (VWR Chemicals, Radnor, PA, USA) was used to keep the pH at 6.5, HEPES buffer (PanReach, AppliChem, Chicago, IL, USA) was used to keep the pH at 8, and, finally, CHES buffer (PanReach, AppliChem, Chicago, IL, USA) was used to keep the pH at 9.5. .. The pH was duly adjusted at the beginning of each experiment, with a tolerance range of ±0.5, and was periodically monitored (on days 0, 1, 4, 6, 8, 11, 13, 15, 18, 20, and 22) with a pH meter (Hanna Instruments, model HI2210, Woonsocket, RI, USA).



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