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CombiMatrix array cgh
Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
Array Cgh, supplied by CombiMatrix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cgh+array/pmc03751902-83-3-14?v=CombiMatrix
Average 90 stars, based on 1 article reviews
array cgh - by Bioz Stars, 2026-07
90/100 stars

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1) Product Images from "Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities"

Article Title: Comprehensive characterization of malignant phyllodes tumor by whole genomic and proteomic analysis: biological implications for targeted therapy opportunities

Journal: Orphanet Journal of Rare Diseases

doi: 10.1186/1750-1172-8-112

Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma
Figure Legend Snippet: Summary of molecular profiling analyses from various CLIA certified methods in a patient with malignant phyllodes sarcoma

Techniques Used: Sequencing, Hybridization, Immunohistochemistry, Staining

Imaging studies, histopathology and FISH studies: UA and B: CT of the chest showing metastatic masses in the lung. C Hematoxylin and Eosin stained section of the tumor from the right mastectomy shows a malignant sarcoma/malignant phyllodes tumor that has high stromal cellularity and a mitotic index of 11 per 10 high power fields. D HER - 2 / neu by Fluorescence in Situ Hybridization (FISH) HER-2 gene amplification was assessed utilizing the PathVysion assay (Vysis Corp., Downers Grove, Illinois). The identification probes for the HER-2 (SpectrumOrange) and alpha satellite DNA sequence at the centromeric region of chromosome 17 (SpectrumGreen) were hybridized according to the manufacturer’s guidelines. At least twenty non-overlapping nuclei containing at least one orange and one green signal were enumerated. The ratio of orange signals (HER-2 gene) to green signals (chromosome 17) was calculated. A ratio greater than or equal to 2.0 is considered as amplified based on the FDA approval in this kit. The CAP HER-2 consensus conference 2002 suggested that a ratio of 1.8-2.2 be considered as borderline.
Figure Legend Snippet: Imaging studies, histopathology and FISH studies: UA and B: CT of the chest showing metastatic masses in the lung. C Hematoxylin and Eosin stained section of the tumor from the right mastectomy shows a malignant sarcoma/malignant phyllodes tumor that has high stromal cellularity and a mitotic index of 11 per 10 high power fields. D HER - 2 / neu by Fluorescence in Situ Hybridization (FISH) HER-2 gene amplification was assessed utilizing the PathVysion assay (Vysis Corp., Downers Grove, Illinois). The identification probes for the HER-2 (SpectrumOrange) and alpha satellite DNA sequence at the centromeric region of chromosome 17 (SpectrumGreen) were hybridized according to the manufacturer’s guidelines. At least twenty non-overlapping nuclei containing at least one orange and one green signal were enumerated. The ratio of orange signals (HER-2 gene) to green signals (chromosome 17) was calculated. A ratio greater than or equal to 2.0 is considered as amplified based on the FDA approval in this kit. The CAP HER-2 consensus conference 2002 suggested that a ratio of 1.8-2.2 be considered as borderline.

Techniques Used: Imaging, Histopathology, Staining, Fluorescence, In Situ Hybridization, Amplification, Sequencing



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