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cfps kit rts 100 e. coli hy  (biotechrabbit)

 
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    biotechrabbit cfps kit rts 100 e. coli hy
    Cfps Kit Rts 100 E. Coli Hy, supplied by biotechrabbit, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cfps+kit/rts+100+e++coli+hy+kit/pm39957478-90-3-13
    Average 90 stars, based on 1 article reviews
    cfps kit rts 100 e. coli hy - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Plasmid Preparation:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    In Vitro:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Microarray:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Inhibition:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Luciferase:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Expressing:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Polymerase Chain Reaction:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Control:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Concentration Assay:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].

    Produced:

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP‐His concentration of 0.014 ± 0.001 mg mL −1 for CFPS from the linear DNA template after 4 h ( Figure ).

    Article Title: Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 n m PCR product or 5 n m plasmid DNA as a DNA template and no DNA as control.

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: The RTS 100 E. coli HY Kit (biotechrabbit GmbH, Berlin, Germany) was used by following the instructions provided by the manufacturer and using 5 nm PCR product or 5 nm plasmid DNA as a DNA template and no DNA as control.

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma
    Article Snippet: Proteins encoded by each purified plasmid were expressed in vitro (RTS 100 E. coli HY kit – Biotechrabbit, Berlin, Germany) according to the manufacturer’s instructions and printed onto eight-pad nitrocellulose-coated AVID glass slides (Grace Biolabs, Bend, OR, USA) with an Omnigrid 100 microarray printer (Genomic Solutions, Ann Arbor, MI, USA).

    Article Title: Regulating Protein Immobilization During Cell-Free Protein Synthesis in Hyaluronan Microgels.
    Article Snippet: First, a commercial CFPS kit, namely RTS 100 E. coli HY kit from biotechrabbit GmbH, was used, which showed a GFP-His concentration of 0.014 ± 0.001 mg mL−1 for CFPS from the linear DNA template after 4 h (Figure 3).

    Article Title: Immunomics-guided biomarker discovery for human liver fluke infection and infection-associated cholangiocarcinoma.
    Article Snippet: The ES products and MV mass spectrometry proteomics data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifiers PXD056031 and PXD02035, respectively.

    Article Title: Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System.
    Article Snippet: We tested this dialysis with lysate reactions (RTS 500 E. coli HY (Biotechrabbit GmbH)) and PURE reactions (PURExpress (NEB)) combined with a homemade energy solution.

    Article Title: Designing New Chimeric Proline-Rich Antimicrobial Peptides to Enhance Efficacy Toward the ESKAPE+E: Beyond Sequence Extension.
    Article Snippet: To assess translation inhibition by the compounds in this study, an E. coli cell-free lysate-based translation system (RTS100 E. coli HY; biotechrabbit) was employed for firefly luciferase (fluc) expression, as previously described for other translation-inhibiting antibiotics [24].



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    Image Search Results


    SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a protein synthesis template in the CFPS reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.

    Journal: Heliyon

    Article Title: Immunoproteomic approach identifies a putative virulence chaperone DnaK protein as a candidate diagnostic marker and therapeutic target for Pythium insidiosum infection

    doi: 10.1016/j.heliyon.2025.e42487

    Figure Lengend Snippet: SDS-PAGE and Western blot analysis of P. insidiosum 's synthesized proteins. Nineteen coding sequences of the recruited target proteins S01-S21 (except S03 and S04) serve as a protein synthesis template in the CFPS reaction, and the resulting products are checked for their presence using SDS-PAGE stained with Coomassie blue dye ( A ) and their immunoreactivity using Western blot analysis probed against the mouse anti-6x-His tag antibody ( B ). Green fluorescent protein (GFP) is a positive control for the CFPS reaction. An arrowhead indicates a target synthesized protein. The molecular weight markers (M) are shown on the left of each figure. The original unprocessed images of are available as supplementary files: Original_Fig2A_S01-02_S11-S14_GFP.tif, Original_Fig2A_S05-06.tif, Original_Fig2A_S07-10.tif, Original_Fig2A_S015-21.tif, Original_Fig2B_S01-S02_S11-S14_GFP.tif, Original_Fig2B_S05-06.tif, Original_Fig2B_S07-10.tif, and Original_Fig2B_S015-21.tif.

    Article Snippet: Up to 16 different proteins can be synthesized simultaneously using a second-round PCR product as a template for protein synthesis and a commercial CFPS kit from Bioneer with an automated protein synthesis machine from the same company.

    Techniques: SDS Page, Western Blot, Synthesized, Staining, Positive Control, Molecular Weight