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lv200 luminescent microscope  (Olympus)


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    Structured Review

    Olympus lv200 luminescent microscope
    a, b Time-lapse images of the HES7 promoter-luciferase reporter activity in a representative somitoid. The starting point on day 4 was defined as time 0. See also Supplementary Movie . Scale bars: 500 µm. Images were taken by an <t>LV200</t> luminescent microscope. b Enlarged images of several time points are also shown. Arrows indicate the somite boundaries. BF: Bright field. c Kymograph of the HES7 reporter activity measured along the posterior-anterior axis (the white arrow) of the same sample shown in a , b . d Detrended intensity (top) and the oscillation phase (bottom) of the HES7 reporter activity in the same sample shown in a , b . Blue and orange lines indicate the signals measured in the posterior and anterior regions of somitoids, respectively, marked in c . The gaps in the graph correspond to short halts of imaging to adjust the sample position. N = 14 samples showed similar oscillatory patterns. e Relationship between HES7 oscillations and segmentation timings. Detrended HES7 reporter activity of the entire image of the same sample shown in a and b. Arrows indicate the timings of somite formation, and the colors correspond to the arrows in b . f Periods calculated from the HES7 oscillations and somite formation. Mean ± SEM. Each point in the graph indicates an average period of several oscillation peaks or somite formation timings in one sample from day 5 to day 6. N = 14 (HES7 oscillation) and 17 (Somite formation). 5 independent experiments. P -value is from two-sided student’s t-test. Source data are provided as a Source Data file.
    Lv200 Luminescent Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 95/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellsens+imaging+software+program/LV200+Bioluminescence+Imaging+System/pmc09050736-307-6-9
    Average 95 stars, based on 236 article reviews
    lv200 luminescent microscope - by Bioz Stars, 2026-09
    95/100 stars

    Images

    1) Product Images from "Periodic formation of epithelial somites from human pluripotent stem cells"

    Article Title: Periodic formation of epithelial somites from human pluripotent stem cells

    Journal: Nature Communications

    doi: 10.1038/s41467-022-29967-1

    a, b Time-lapse images of the HES7 promoter-luciferase reporter activity in a representative somitoid. The starting point on day 4 was defined as time 0. See also Supplementary Movie . Scale bars: 500 µm. Images were taken by an LV200 luminescent microscope. b Enlarged images of several time points are also shown. Arrows indicate the somite boundaries. BF: Bright field. c Kymograph of the HES7 reporter activity measured along the posterior-anterior axis (the white arrow) of the same sample shown in a , b . d Detrended intensity (top) and the oscillation phase (bottom) of the HES7 reporter activity in the same sample shown in a , b . Blue and orange lines indicate the signals measured in the posterior and anterior regions of somitoids, respectively, marked in c . The gaps in the graph correspond to short halts of imaging to adjust the sample position. N = 14 samples showed similar oscillatory patterns. e Relationship between HES7 oscillations and segmentation timings. Detrended HES7 reporter activity of the entire image of the same sample shown in a and b. Arrows indicate the timings of somite formation, and the colors correspond to the arrows in b . f Periods calculated from the HES7 oscillations and somite formation. Mean ± SEM. Each point in the graph indicates an average period of several oscillation peaks or somite formation timings in one sample from day 5 to day 6. N = 14 (HES7 oscillation) and 17 (Somite formation). 5 independent experiments. P -value is from two-sided student’s t-test. Source data are provided as a Source Data file.
    Figure Legend Snippet: a, b Time-lapse images of the HES7 promoter-luciferase reporter activity in a representative somitoid. The starting point on day 4 was defined as time 0. See also Supplementary Movie . Scale bars: 500 µm. Images were taken by an LV200 luminescent microscope. b Enlarged images of several time points are also shown. Arrows indicate the somite boundaries. BF: Bright field. c Kymograph of the HES7 reporter activity measured along the posterior-anterior axis (the white arrow) of the same sample shown in a , b . d Detrended intensity (top) and the oscillation phase (bottom) of the HES7 reporter activity in the same sample shown in a , b . Blue and orange lines indicate the signals measured in the posterior and anterior regions of somitoids, respectively, marked in c . The gaps in the graph correspond to short halts of imaging to adjust the sample position. N = 14 samples showed similar oscillatory patterns. e Relationship between HES7 oscillations and segmentation timings. Detrended HES7 reporter activity of the entire image of the same sample shown in a and b. Arrows indicate the timings of somite formation, and the colors correspond to the arrows in b . f Periods calculated from the HES7 oscillations and somite formation. Mean ± SEM. Each point in the graph indicates an average period of several oscillation peaks or somite formation timings in one sample from day 5 to day 6. N = 14 (HES7 oscillation) and 17 (Somite formation). 5 independent experiments. P -value is from two-sided student’s t-test. Source data are provided as a Source Data file.

    Techniques Used: Luciferase, Activity Assay, Microscopy, Imaging

    Related Articles

    Microscopy:

    Article Title: NAD + controls circadian rhythmicity during cardiac aging.
    Article Snippet: .. Microscopic real-time bioluminescence analysis Bioluminescence from adult PER2::luc cardiomyocytes was assessed with an LV200 microscope (Olympus) in a humidified chamber under 5% CO2, at 37°C guided by a controller (Tokahit), using a 10x0.3NA Plan Semi Apo objective (Olympus). .. Cells were synchronized with 100 nM dexamethasone for 2h and changed to adult cardiomyocyte culture medium, containing 1mM D-Luciferin Potassium Salt (Promega).

    Article Title: NAD + controls circadian rhythmicity during cardiac aging
    Article Snippet: .. Bioluminescence from adult PER2::luc cardiomyocytes was assessed with an LV200 microscope (Olympus) in a humidified chamber under 5% CO 2 , at 37 °C guided by a controller (Tokahit), using a 10×0.3NA Plan Semi Apo objective (Olympus). .. Cells were synchronized with 100 nM dexamethasone for 2 h and changed to adult cardiomyocyte culture medium, containing 1 mM D-Luciferin Potassium Salt (Promega).

    Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock
    Article Snippet: To block the Na + channels, 1 μM tetrodotoxin (Sigma) was present in the recording medium before treatment with DEX. .. To image the PER2 luminescence signal at the microscopic level, 14 explants were placed in the motorized luminescence microscope Luminoview LV200 (Olympus, Japan) with LUCPLFLN40X objective (Olympus) and the EMCCD camera ImageEM X2 (Hamamatsu, Japan) water cooled by Minichiller 280 (Huber, Germany); luminescence signal was imaged every hour with an exposure time of 10 min and 250 electromagnetic gain. .. One micromole of tetrodotoxin (Sigma) was present in the recording medium to block the Na + channels; to avoid affecting the position of the explant above the objective, instead of adding DEX ( n = 7) or VEH ( n = 7) to the medium after 3 days of imaging, 5 μL of pre‐diluted DEX (200 nM) or VEH was dropped directly on top of the explant.

    Article Title:
    Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Olympus LV200 bioluminescence microscope using a 60× objective (FIGS. 80A-B) for 25, 100, 1000, and 5000 ms. To analyze subcellular localization, N-terminal L27V fusions with the GPCR AT1R (Angiotensin type 1 receptor (SEQ ID NOs: 459 and 460)) with IL-6 secretion sequence (SEQ ID NOs: 461 and 462) or the transcription factor, Nrf2 (SEQ ID NO: 317), were made using a GSSG linker (SEQ ID NOs: 457 and 458) and transfected into U2OS cells as described above (FIGS. 81A-C). ..

    Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock
    Article Snippet: .. To reveal the dynamics of the response to DEX in TTX‐treated adult SCN at the single‐cell level, we used luminescence microscopy (Olympus LV200) and analyzed PER2 levels in approximately cell‐sized regions of interest (rois) recorded every 1 h for 72 h before explants were temporarily treated with either 200 nM DEX or VEH (a drop on top of the explant, Figure ). ..

    Imaging:

    Article Title: Transient molecular chimerism for exploiting xenogeneic organelles.
    Article Snippet: .. For luminescence imaging, the cells were briefly suspended in 100% methanol to immobilize them and increase substrate permeability, and then they were resuspended in IMK medium before imaging with a luminescence imaging system (LV200, Olympus). ..

    Article Title: Transient molecular chimerism for exploiting xenogeneic organelles
    Article Snippet: .. For luminescence imaging, the cells were briefly suspended in 100% methanol to immobilize them and increase substrate permeability, and then they were resuspended in IMK medium before imaging with a luminescence imaging system (LV200, Olympus). ..

    Permeability:

    Article Title: Transient molecular chimerism for exploiting xenogeneic organelles.
    Article Snippet: .. For luminescence imaging, the cells were briefly suspended in 100% methanol to immobilize them and increase substrate permeability, and then they were resuspended in IMK medium before imaging with a luminescence imaging system (LV200, Olympus). ..

    Article Title: Transient molecular chimerism for exploiting xenogeneic organelles
    Article Snippet: .. For luminescence imaging, the cells were briefly suspended in 100% methanol to immobilize them and increase substrate permeability, and then they were resuspended in IMK medium before imaging with a luminescence imaging system (LV200, Olympus). ..

    Incubation:

    Article Title:
    Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Olympus LV200 bioluminescence microscope using a 60× objective (FIGS. 80A-B) for 25, 100, 1000, and 5000 ms. To analyze subcellular localization, N-terminal L27V fusions with the GPCR AT1R (Angiotensin type 1 receptor (SEQ ID NOs: 459 and 460)) with IL-6 secretion sequence (SEQ ID NOs: 461 and 462) or the transcription factor, Nrf2 (SEQ ID NO: 317), were made using a GSSG linker (SEQ ID NOs: 457 and 458) and transfected into U2OS cells as described above (FIGS. 81A-C). ..

    Sequencing:

    Article Title:
    Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Olympus LV200 bioluminescence microscope using a 60× objective (FIGS. 80A-B) for 25, 100, 1000, and 5000 ms. To analyze subcellular localization, N-terminal L27V fusions with the GPCR AT1R (Angiotensin type 1 receptor (SEQ ID NOs: 459 and 460)) with IL-6 secretion sequence (SEQ ID NOs: 461 and 462) or the transcription factor, Nrf2 (SEQ ID NO: 317), were made using a GSSG linker (SEQ ID NOs: 457 and 458) and transfected into U2OS cells as described above (FIGS. 81A-C). ..

    Transfection:

    Article Title:
    Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Olympus LV200 bioluminescence microscope using a 60× objective (FIGS. 80A-B) for 25, 100, 1000, and 5000 ms. To analyze subcellular localization, N-terminal L27V fusions with the GPCR AT1R (Angiotensin type 1 receptor (SEQ ID NOs: 459 and 460)) with IL-6 secretion sequence (SEQ ID NOs: 461 and 462) or the transcription factor, Nrf2 (SEQ ID NO: 317), were made using a GSSG linker (SEQ ID NOs: 457 and 458) and transfected into U2OS cells as described above (FIGS. 81A-C). ..

    other:

    Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock
    Article Snippet: CellSens Dimensions (Olympus) was used to acquire LV200 images and export them as 16‐bit TIFFs.

    Single Cell:

    Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock
    Article Snippet: .. To reveal the dynamics of the response to DEX in TTX‐treated adult SCN at the single‐cell level, we used luminescence microscopy (Olympus LV200) and analyzed PER2 levels in approximately cell‐sized regions of interest (rois) recorded every 1 h for 72 h before explants were temporarily treated with either 200 nM DEX or VEH (a drop on top of the explant, Figure ). ..



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    TaWAKL8-2B positively regulates wheat resistance to Pst. A Disease phenotype of TaWAKL8 - 2B -KO plants inoculated with Pst CYR23. Representative images of disease phenotype were taken at 14 dpi. B Relative fungal biomass in TaWAKL8-2B -KO plants. The relative biomass ratio ( Pst /wheat) was assayed with DNA isolated from the leaves at 14 dpi. TaEF-1α and PstEF were used to normalize the DNA level of wheat and Pst , respectively. C Relative transcription levels of TaPR1 , TaPR2 and TaPR5 in TaWAKL8 - 2B -KO plants inoculated with CYR23. D H 2 O 2 accumulation at the infection sites in TaWAKL8 - 2B -KO plants stained with DAB at 24 and 48 hpi. E Quantification of tissue areas containing H 2 O 2 from D . F Histological observation of Pst growth and development in TaWAKL8 - 2B -KO and Fielder plants. Pst infection structures were stained with wheat germ agglutinin conjugated to Alexa-488 and observed with an Olympus BX-53 microscope. SV, substomatal vesicle; IH, infection hypha; HMC, haustorial mother cell; H, haustorium; (g) Hyphal length was measured by <t>Cellsens</t> software. Values in ( E and G ) represent the mean ± SEM from three independent samples with 90 infection sites. Error bars in ( B and C ) indicate SD from three technical replicates. Experiments were independently repeated three times with similar results. Asterisks indicate significant differences (Student’s t test, * p < 0.05, ** p < 0.01, **** p < 0.0001)
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    Image Search Results


    TaWAKL8-2B positively regulates wheat resistance to Pst. A Disease phenotype of TaWAKL8 - 2B -KO plants inoculated with Pst CYR23. Representative images of disease phenotype were taken at 14 dpi. B Relative fungal biomass in TaWAKL8-2B -KO plants. The relative biomass ratio ( Pst /wheat) was assayed with DNA isolated from the leaves at 14 dpi. TaEF-1α and PstEF were used to normalize the DNA level of wheat and Pst , respectively. C Relative transcription levels of TaPR1 , TaPR2 and TaPR5 in TaWAKL8 - 2B -KO plants inoculated with CYR23. D H 2 O 2 accumulation at the infection sites in TaWAKL8 - 2B -KO plants stained with DAB at 24 and 48 hpi. E Quantification of tissue areas containing H 2 O 2 from D . F Histological observation of Pst growth and development in TaWAKL8 - 2B -KO and Fielder plants. Pst infection structures were stained with wheat germ agglutinin conjugated to Alexa-488 and observed with an Olympus BX-53 microscope. SV, substomatal vesicle; IH, infection hypha; HMC, haustorial mother cell; H, haustorium; (g) Hyphal length was measured by Cellsens software. Values in ( E and G ) represent the mean ± SEM from three independent samples with 90 infection sites. Error bars in ( B and C ) indicate SD from three technical replicates. Experiments were independently repeated three times with similar results. Asterisks indicate significant differences (Student’s t test, * p < 0.05, ** p < 0.01, **** p < 0.0001)

    Journal: Stress Biology

    Article Title: TaWAKL8-2B, a wall-associated receptor-like kinase, mediates wheat rust resistance by linalool and ROS accumulation

    doi: 10.1007/s44154-025-00248-3

    Figure Lengend Snippet: TaWAKL8-2B positively regulates wheat resistance to Pst. A Disease phenotype of TaWAKL8 - 2B -KO plants inoculated with Pst CYR23. Representative images of disease phenotype were taken at 14 dpi. B Relative fungal biomass in TaWAKL8-2B -KO plants. The relative biomass ratio ( Pst /wheat) was assayed with DNA isolated from the leaves at 14 dpi. TaEF-1α and PstEF were used to normalize the DNA level of wheat and Pst , respectively. C Relative transcription levels of TaPR1 , TaPR2 and TaPR5 in TaWAKL8 - 2B -KO plants inoculated with CYR23. D H 2 O 2 accumulation at the infection sites in TaWAKL8 - 2B -KO plants stained with DAB at 24 and 48 hpi. E Quantification of tissue areas containing H 2 O 2 from D . F Histological observation of Pst growth and development in TaWAKL8 - 2B -KO and Fielder plants. Pst infection structures were stained with wheat germ agglutinin conjugated to Alexa-488 and observed with an Olympus BX-53 microscope. SV, substomatal vesicle; IH, infection hypha; HMC, haustorial mother cell; H, haustorium; (g) Hyphal length was measured by Cellsens software. Values in ( E and G ) represent the mean ± SEM from three independent samples with 90 infection sites. Error bars in ( B and C ) indicate SD from three technical replicates. Experiments were independently repeated three times with similar results. Asterisks indicate significant differences (Student’s t test, * p < 0.05, ** p < 0.01, **** p < 0.0001)

    Article Snippet: Quantification of H 2 O 2 -containing areas and Pst growth were performed with the cellSens Entry software program (Olympus, Tokyo, Japan).

    Techniques: Isolation, Infection, Staining, Microscopy, Software