lv200 luminescent microscope (Olympus)
Structured Review

Lv200 Luminescent Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 95/100, based on 236 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cellsens+imaging+software+program/LV200+Bioluminescence+Imaging+System/pmc09050736-307-6-9
Average 95 stars, based on 236 article reviews
Images
1) Product Images from "Periodic formation of epithelial somites from human pluripotent stem cells"
Article Title: Periodic formation of epithelial somites from human pluripotent stem cells
Journal: Nature Communications
doi: 10.1038/s41467-022-29967-1
Figure Legend Snippet: a, b Time-lapse images of the HES7 promoter-luciferase reporter activity in a representative somitoid. The starting point on day 4 was defined as time 0. See also Supplementary Movie . Scale bars: 500 µm. Images were taken by an LV200 luminescent microscope. b Enlarged images of several time points are also shown. Arrows indicate the somite boundaries. BF: Bright field. c Kymograph of the HES7 reporter activity measured along the posterior-anterior axis (the white arrow) of the same sample shown in a , b . d Detrended intensity (top) and the oscillation phase (bottom) of the HES7 reporter activity in the same sample shown in a , b . Blue and orange lines indicate the signals measured in the posterior and anterior regions of somitoids, respectively, marked in c . The gaps in the graph correspond to short halts of imaging to adjust the sample position. N = 14 samples showed similar oscillatory patterns. e Relationship between HES7 oscillations and segmentation timings. Detrended HES7 reporter activity of the entire image of the same sample shown in a and b. Arrows indicate the timings of somite formation, and the colors correspond to the arrows in b . f Periods calculated from the HES7 oscillations and somite formation. Mean ± SEM. Each point in the graph indicates an average period of several oscillation peaks or somite formation timings in one sample from day 5 to day 6. N = 14 (HES7 oscillation) and 17 (Somite formation). 5 independent experiments. P -value is from two-sided student’s t-test. Source data are provided as a Source Data file.
Techniques Used: Luciferase, Activity Assay, Microscopy, Imaging
Related Articles
Microscopy:Article Title: NAD + controls circadian rhythmicity during cardiac aging. Article Snippet: .. Microscopic real-time bioluminescence analysis Bioluminescence from adult PER2::luc cardiomyocytes was assessed with an Article Title: NAD + controls circadian rhythmicity during cardiac aging Article Snippet: .. Bioluminescence from adult PER2::luc cardiomyocytes was assessed with an Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock Article Snippet: To block the Na + channels, 1 μM tetrodotoxin (Sigma) was present in the recording medium before treatment with DEX. .. To image the PER2 luminescence signal at the microscopic level, 14 explants were placed in the motorized Article Title: Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock Article Snippet: .. To reveal the dynamics of the response to DEX in TTX‐treated adult SCN at the single‐cell level, we used Imaging:Article Title: Transient molecular chimerism for exploiting xenogeneic organelles. Article Snippet: .. For Article Title: Transient molecular chimerism for exploiting xenogeneic organelles Article Snippet: .. For Permeability:Article Title: Transient molecular chimerism for exploiting xenogeneic organelles. Article Snippet: .. For Article Title: Transient molecular chimerism for exploiting xenogeneic organelles Article Snippet: .. For Incubation:Article Title: Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Sequencing:Article Title: Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an Transfection:Article Title: Article Snippet: The reaction mixture was incubated at 30° C. for 120 min. An OgLuc activity assay was performed by adding to the 50 μL OgLuc translation mixture, 50 μL OgLuc Glo Reagent (100 mM MES (pH 6.0), 1 mM CDTA, 150 mM KCl, 35 mM thiourea, 2 mM DTT, 0.25% TERGITOL NP-9 (v/v), 0.025% MAZU DF 204, and 20 μM PBI-3939) with or without 100 μM cAMP, and performing a kinetic read for 30 min (TECAN INFINITE F500 Plate Reader). .. After a 30 min incubation at 37° C., unfiltered images were captured on an other:Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock Article Snippet: Single Cell:Article Title: Suprachiasmatic Nuclei Possess Glucocorticoid Receptors That Activate Downstream Signaling Pathways but Do Not Entrain Their Circadian Clock Article Snippet: .. To reveal the dynamics of the response to DEX in TTX‐treated adult SCN at the single‐cell level, we used |
