cellsens digital imaging software  (Carl Zeiss)

Journal: Stress Biology

Article Title: TaWAKL8-2B, a wall-associated receptor-like kinase, mediates wheat rust resistance by linalool and ROS accumulation

doi: 10.1007/s44154-025-00248-3

Figure Lengend Snippet: TaWAKL8-2B positively regulates wheat resistance to Pst. A Disease phenotype of TaWAKL8 - 2B -KO plants inoculated with Pst CYR23. Representative images of disease phenotype were taken at 14 dpi. B Relative fungal biomass in TaWAKL8-2B -KO plants. The relative biomass ratio ( Pst /wheat) was assayed with DNA isolated from the leaves at 14 dpi. TaEF-1α and PstEF were used to normalize the DNA level of wheat and Pst , respectively. C Relative transcription levels of TaPR1 , TaPR2 and TaPR5 in TaWAKL8 - 2B -KO plants inoculated with CYR23. D H 2 O 2 accumulation at the infection sites in TaWAKL8 - 2B -KO plants stained with DAB at 24 and 48 hpi. E Quantification of tissue areas containing H 2 O 2 from D . F Histological observation of Pst growth and development in TaWAKL8 - 2B -KO and Fielder plants. Pst infection structures were stained with wheat germ agglutinin conjugated to Alexa-488 and observed with an Olympus BX-53 microscope. SV, substomatal vesicle; IH, infection hypha; HMC, haustorial mother cell; H, haustorium; (g) Hyphal length was measured by Cellsens software. Values in ( E and G ) represent the mean ± SEM from three independent samples with 90 infection sites. Error bars in ( B and C ) indicate SD from three technical replicates. Experiments were independently repeated three times with similar results. Asterisks indicate significant differences (Student’s t test, * p < 0.05, ** p < 0.01, **** p < 0.0001)

Article Snippet: Quantification of H 2 O 2 -containing areas and Pst growth were performed with the cellSens Entry software program (Olympus, Tokyo, Japan).

Techniques: Isolation, Infection, Staining, Microscopy, Software