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qiaamp viral rna mini kit  (Qiagen)


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    Qiagen qiaamp viral rna mini kit
    Summary table of available protocols posted to the WHO’s website.
    Qiaamp Viral Rna Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 5497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+transcriptomic+sequencing+dataset/QIAamp+Viral+RNA+Mini+Kit/pmc07354519-149-73-78
    Average 99 stars, based on 5497 article reviews
    qiaamp viral rna mini kit - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2"

    Article Title: Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

    Journal: Viruses

    doi: 10.3390/v12060582

    Summary table of available protocols posted to the WHO’s website.
    Figure Legend Snippet: Summary table of available protocols posted to the WHO’s website.

    Techniques Used: Amplification, Concentration Assay, RNA Extraction, Virus, Sequencing, Extraction, Diagnostic Assay, Positive Control, Reverse Transcription, Reverse Transcription Polymerase Chain Reaction, One Step RT-PCR, Cell Culture

    Related Articles

    Isolation:

    Article Title: Host species-specific mutations in the thumb domain of the 3D pol polymerase are required for efficient replication of human hepatitis A virus in mice
    Article Snippet: Total RNA was extracted from cultured cells using the RNeasy Kit (Qiagen, #74104), and from mouse liver tissue using TRIzol reagent (ThermoFisher, #15596026). .. Fecal RNA was isolated using the QIAamp Viral RNA Isolation Kit (Qiagen, #52906). cDNA synthesis was carried out using the SuperScript III First-Strand Synthesis System (ThermoFisher, #18080051). .. Primers targeting the 5’ untranslated region of the HAV genome were used to quantify HAV RNA genome equivalents (GE) in a SYBR Green Real-Time qPCR assay (BioRad, #1725121) against a synthetic RNA standard, as described previously [ ].

    Article Title: Host species-specific mutations in the thumb domain of the 3Dpol polymerase are required for efficient replication of human hepatitis A virus in mice.
    Article Snippet: Antibodies used in this study included: rabbit monoclonal anti-HA-Tag, C29F4 (Cell Signaling Technology, #3724); recombinant rabbit anti-Hsc70 (HSPA8) (Proteintech, #81719–2-RR); rabbit anti-BAG2 (Proteintech, #29820–1-AP); rabbit anti-hnRNPH1 (Proteintech, #14774–1-AP); rabbit anti-RACK1 (Proteintech, 27592–1-AP); rabbit anti-Ku70 (XRCC6), a gift from Dale Ramsden, The University of North Carolina at Chapel Hill; recombinant rabbit anti-ANT1/2 (SLC25A5) (Proteintech, #83104.1); rabbit monoclonal anti-GAPDH (14C10) (Cell Signaling Technologies, #2118); rabbit anti-β-actin (Cell Signaling Technologies, #4967); and, IRDye 800CW donkey anti-rabbit IgG (LICOR, #926–32213). .. Fecal RNA was isolated using the QIAamp Viral RNA Isolation Kit (Qiagen, #52906). cDNA synthesis was carried out using the SuperScript III First-Strand Synthesis System (ThermoFisher, #18080051). .. The S-isomer of the antiviral compound RG7834 was purchased from MedChemExpress (#HY-117650A), and used to assess HAV replication at a concentration of 200nM.

    cDNA Synthesis:

    Article Title: Host species-specific mutations in the thumb domain of the 3D pol polymerase are required for efficient replication of human hepatitis A virus in mice
    Article Snippet: Total RNA was extracted from cultured cells using the RNeasy Kit (Qiagen, #74104), and from mouse liver tissue using TRIzol reagent (ThermoFisher, #15596026). .. Fecal RNA was isolated using the QIAamp Viral RNA Isolation Kit (Qiagen, #52906). cDNA synthesis was carried out using the SuperScript III First-Strand Synthesis System (ThermoFisher, #18080051). .. Primers targeting the 5’ untranslated region of the HAV genome were used to quantify HAV RNA genome equivalents (GE) in a SYBR Green Real-Time qPCR assay (BioRad, #1725121) against a synthetic RNA standard, as described previously [ ].

    Article Title: Host species-specific mutations in the thumb domain of the 3Dpol polymerase are required for efficient replication of human hepatitis A virus in mice.
    Article Snippet: Antibodies used in this study included: rabbit monoclonal anti-HA-Tag, C29F4 (Cell Signaling Technology, #3724); recombinant rabbit anti-Hsc70 (HSPA8) (Proteintech, #81719–2-RR); rabbit anti-BAG2 (Proteintech, #29820–1-AP); rabbit anti-hnRNPH1 (Proteintech, #14774–1-AP); rabbit anti-RACK1 (Proteintech, 27592–1-AP); rabbit anti-Ku70 (XRCC6), a gift from Dale Ramsden, The University of North Carolina at Chapel Hill; recombinant rabbit anti-ANT1/2 (SLC25A5) (Proteintech, #83104.1); rabbit monoclonal anti-GAPDH (14C10) (Cell Signaling Technologies, #2118); rabbit anti-β-actin (Cell Signaling Technologies, #4967); and, IRDye 800CW donkey anti-rabbit IgG (LICOR, #926–32213). .. Fecal RNA was isolated using the QIAamp Viral RNA Isolation Kit (Qiagen, #52906). cDNA synthesis was carried out using the SuperScript III First-Strand Synthesis System (ThermoFisher, #18080051). .. The S-isomer of the antiviral compound RG7834 was purchased from MedChemExpress (#HY-117650A), and used to assess HAV replication at a concentration of 200nM.

    Digital PCR:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Extraction:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Amplification:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Countercurrent Chromatography:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Reverse Transcription:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: .. 6.11.4 Viral RNA Extraction and Reverse Transcription Quantitative PCR Viral RNA was extracted by Viral RNA Mini Kit (QIAGEN, Cat #52906, USA) according to the manufacturer's instructions. .. Extracted RNA were treated with DNase I (QIAGEN, Cat #79254, USA) endonuclease according to the manufacturer's protocol and purified by RNeasy Mini Kit (QIAGEN, Cat #74106, USA) for RNA Purification to exclude plasmid DNA contamination.

    Concentration Assay:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Control:

    Article Title: Filamentous morphology of influenza A virus confers enhanced stability in aerosols.
    Article Snippet: .. Quantification of IAV genome copies by dPCR RNA was extracted by QIAamp Viral RNA Mini extraction kit (Qiagen, 52906) and eluted in 80 μL of elution buffer following manufacturer’s instructions. dPCR was performed using the QIAcuity Digital PCR System and QIAcuity OneStep Advanced Probe Kit AR TI CL E IN P RE SS (#250132, Qiagen) with the following primers targeting a 110-base amplicon of the IAV M segment (Microsynth): Forward primer 5’- TGG AAT GGC TAA AGA CAA GAC CAA T-3’ Reverse primer 5’- AAA GCG TCT ACG CTG CAG TCC-3’ Probe- TTT GTK TTC ACG CTC ACC GTG CCC A 12 μL single reaction mixture was prepared with 3 μL 4× OneStep Advanced Probe Mastermix, 0.12 μL 100× OneStep Advanced reverse transcriptase (RT) Mix, 1.2 μL Primer-Probe working solution (0.8 μM final concentration of each primer and 0.4 μM final concentration for probe), 1.5 μL Enhancer GC, 3.18 μL RNase-free water, and 3 μL of RNA sample diluted within dPCR detection range (<104 copies/μL) or non-template control. .. The reactions were transferred into QIAcuity Nanoplate 8.5k 24-well or 96-well (#250011 or #250021, Qiagen) and dPCR was conducted by QIAcuity One Digital PCR System (#911020, QIAcuity one, 5-plex, Qiagen).

    Virus:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    Recombinant:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    FACS:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    Flow Cytometry:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    Plasmid Preparation:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    Quantitative RT-PCR:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    Generated:

    Article Title: An atlas of sarbecovirus infectivity across diverse ACE2 orthologs.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies GAPDH Mouse mAb ABclonal Cat# AC033; RRID: AB_2769570 Mouse anti-DDDDK-Tag mAb ABclonal Cat# AE005; RRID: AB_2770401 Mouse anti-His-Tag mAb ABclonal Cat# AE003; RRID: AB_2728734 Anti-VSV-M [23H12] antibody Kerafast Cat# EB0011; RRID: AB_2734773 Bacterial and virus strains G*ΔG vesicular stomatitis virus Kerafast N/A Chemicals, peptides, and recombinant proteins Lipofectamine 3000 Invitrogen Cat# L3000015 High-glucose DMEM HyClone Cat# SH30243.01B Fetal bovine serum HyClone Cat# SH30406.05 Penicillin-streptomycin Gibco Cat# 15140122 PBS HyClone Cat# SH30256.01B 0.05% Trypsin-EDTA Gibco Cat# 25300054 Bright-GloTM PerkinElmer Cat# 6066769 Opti-MEM Gibco Cat# 31985088 PrimeSTAR Max DNA Polymerase Takara Cat# R045B SARS-CoV Spike/RBD Protein Sino Biological Cat# 40150-V05H SARS-CoV-2 (2019-nCoV) Spike RBD-mFc Recombinant Protein Sino Biological Cat# 40592-V05H Human ACE2 Protein Sino Biological Cat# 10108-H08H M. schreibersii ACE2 recombinant protein This paper/Sino Biological N/A BD Shutdown Solution BD Cat# 334224 BD FACS Flow BD Cat# 342003 BD FACS Cleaning Solution BD Cat# 340345 Critical commercial assays QIAamp Viral RNA Mini Kit Qiagen Cat# 52906 QIAGEN Plasmid Plus Maxi Kit Qiagen Cat# 12965 SuperScript III First-Strand Synthesis System and RT-qPCR Kit Invitrogen Cat# 18080–051 TB Green Premix Ex Taq II TaKaRa Cat# RR820A Deposited data Data generated in this study This paper; Figshare https://doi.org/10.6084/m9.figshare. .. 32981705 Sequence datasets used in this study GenBank/NCBI GenBank accession numbers: Tables S1–S4 Experimental models: Cell Lines 293T cells ATCC CRL-3216 BHK-21 cells ATCC CCL-10 Oligonucleotides Miniopterus ACE2 point mutation primer(s) This paper/SinoGenoMax Table S5 Recombinant DNA pcDNA3.1-based sarbecovirus S-expression plasmids This paper/General Biol Table S1 (Continued on next page) 12 iScience 29, 117769, October 16, 2026

    RNA Extraction:

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: .. 6.11.4 Viral RNA Extraction and Reverse Transcription Quantitative PCR Viral RNA was extracted by Viral RNA Mini Kit (QIAGEN, Cat #52906, USA) according to the manufacturer's instructions. .. Extracted RNA were treated with DNase I (QIAGEN, Cat #79254, USA) endonuclease according to the manufacturer's protocol and purified by RNeasy Mini Kit (QIAGEN, Cat #74106, USA) for RNA Purification to exclude plasmid DNA contamination.

    Real-time Polymerase Chain Reaction:

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: .. 6.11.4 Viral RNA Extraction and Reverse Transcription Quantitative PCR Viral RNA was extracted by Viral RNA Mini Kit (QIAGEN, Cat #52906, USA) according to the manufacturer's instructions. .. Extracted RNA were treated with DNase I (QIAGEN, Cat #79254, USA) endonuclease according to the manufacturer's protocol and purified by RNeasy Mini Kit (QIAGEN, Cat #74106, USA) for RNA Purification to exclude plasmid DNA contamination.



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    cell transcriptomic sequencing dataset  (10X Genomics)
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    10X Genomics cell transcriptomic sequencing dataset
    <t>Transcriptomic</t> and TME characteristics associated with ECMSig in TCGA-GBM cohort (A) Volcano plot showing DEGs between ECMSig-high and ECMSig-low groups. Red dots: upregulated in high-risk; blue dots: upregulated in low-risk. Benjamini-Hochberg adjusted. (B) Gene set enrichment analysis (GSEA) plots showing enrichment of hallmark pathways. Pathways enriched in ECMSig-high and ECMSig-low groups are shown with their running enrichment scores (ESs) and ranked gene lists. Benjamini-Hochberg adjusted. (C) Heatmap showing the activity scores of selected oncogenic and tumor-related signaling pathways (rows) across TCGA-GBM samples (columns), annotated by ECMSig group and ECMSig score. Red indicates high activity, blue indicates low activity. ∗ p < 0.05. Wilcoxon signed-rank test. (D) Heatmap depicting the estimated infiltration levels of various immune and stromal cell types (rows) in TCGA-GBM samples (columns), stratified by ECMSig group and score. Red indicates high infiltration, blue indicates low infiltration. Cells significantly highly infiltrated in ECMSig-high are labeled in red, and those high in ECMSig-low group are in blue. ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001. Wilcoxon signed-rank test. Benjamini-Hochberg adjusted. (E and F) Scatterplots showing the spearman correlation between ECMSig score and (E) Macrophage_XCELL infiltration score and (F) immune_score_XCELL. The blue line represents the linear regression fit with 95% confidence interval bands. Spearman correlation test.
    Cell Transcriptomic Sequencing Dataset, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transcriptomic and TME characteristics associated with ECMSig in TCGA-GBM cohort (A) Volcano plot showing DEGs between ECMSig-high and ECMSig-low groups. Red dots: upregulated in high-risk; blue dots: upregulated in low-risk. Benjamini-Hochberg adjusted. (B) Gene set enrichment analysis (GSEA) plots showing enrichment of hallmark pathways. Pathways enriched in ECMSig-high and ECMSig-low groups are shown with their running enrichment scores (ESs) and ranked gene lists. Benjamini-Hochberg adjusted. (C) Heatmap showing the activity scores of selected oncogenic and tumor-related signaling pathways (rows) across TCGA-GBM samples (columns), annotated by ECMSig group and ECMSig score. Red indicates high activity, blue indicates low activity. ∗ p < 0.05. Wilcoxon signed-rank test. (D) Heatmap depicting the estimated infiltration levels of various immune and stromal cell types (rows) in TCGA-GBM samples (columns), stratified by ECMSig group and score. Red indicates high infiltration, blue indicates low infiltration. Cells significantly highly infiltrated in ECMSig-high are labeled in red, and those high in ECMSig-low group are in blue. ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001. Wilcoxon signed-rank test. Benjamini-Hochberg adjusted. (E and F) Scatterplots showing the spearman correlation between ECMSig score and (E) Macrophage_XCELL infiltration score and (F) immune_score_XCELL. The blue line represents the linear regression fit with 95% confidence interval bands. Spearman correlation test.

    Journal: iScience

    Article Title: Multi-omics profiling-derived signature links cellular ecosystem to glioblastoma prognosis

    doi: 10.1016/j.isci.2026.115982

    Figure Lengend Snippet: Transcriptomic and TME characteristics associated with ECMSig in TCGA-GBM cohort (A) Volcano plot showing DEGs between ECMSig-high and ECMSig-low groups. Red dots: upregulated in high-risk; blue dots: upregulated in low-risk. Benjamini-Hochberg adjusted. (B) Gene set enrichment analysis (GSEA) plots showing enrichment of hallmark pathways. Pathways enriched in ECMSig-high and ECMSig-low groups are shown with their running enrichment scores (ESs) and ranked gene lists. Benjamini-Hochberg adjusted. (C) Heatmap showing the activity scores of selected oncogenic and tumor-related signaling pathways (rows) across TCGA-GBM samples (columns), annotated by ECMSig group and ECMSig score. Red indicates high activity, blue indicates low activity. ∗ p < 0.05. Wilcoxon signed-rank test. (D) Heatmap depicting the estimated infiltration levels of various immune and stromal cell types (rows) in TCGA-GBM samples (columns), stratified by ECMSig group and score. Red indicates high infiltration, blue indicates low infiltration. Cells significantly highly infiltrated in ECMSig-high are labeled in red, and those high in ECMSig-low group are in blue. ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001. Wilcoxon signed-rank test. Benjamini-Hochberg adjusted. (E and F) Scatterplots showing the spearman correlation between ECMSig score and (E) Macrophage_XCELL infiltration score and (F) immune_score_XCELL. The blue line represents the linear regression fit with 95% confidence interval bands. Spearman correlation test.

    Article Snippet: The single-cell transcriptomic sequencing dataset utilizing technology from the 10X Genomics platform was available under the accession number GEO: GSE182109 at the Gene Expression Omnibus (GEO) repository.

    Techniques: Activity Assay, Protein-Protein interactions, Labeling

    Single-cell RNA sequencing analysis revealing ECMSig expression across cell types and identification of prognostically relevant cell states in GBM (A) UMAP visualization of major cell types identified in GBM scRNA-seq data. (B) Dot plot showing the scaled average expression (color intensity) and percentage of cells expressing (dot size) canonical marker genes for each major cell type. (C) Dot plot showing the scaled average expression and percentage of cells expressing the seven ECMSig genes across major cell types. (D) UMAP plots showing the expression levels of individual ECMSig genes and overall ECMSig score across all cells. (E–G) UMAP plots illustrating Scissor-identified prognostically unfavorable (Scissor_Pos, red dashed circle) and favorable (Scissor_Neg, blue dashed circle; Scissor_Others, gray) cell subpopulations within (E) tumor cells, (F) myeloid cells, and (G) endothelial cells. (H–K) Violin plots comparing ECMSig scores among tumor cells grouped by Scissor status (H) and tumor type (I), and myeloid cells (J) or endothelial cells (K) grouped by Scissor status. ∗∗∗∗ p < 0.0001. Wilcoxon signed-rank test. (L) Dot plot showing differentially expressed marker genes between myeloid Scissor_Pos and other myeloid cells. Dot size indicates the fraction of cells in the group expressing the gene; color indicates average expression level.

    Journal: iScience

    Article Title: Multi-omics profiling-derived signature links cellular ecosystem to glioblastoma prognosis

    doi: 10.1016/j.isci.2026.115982

    Figure Lengend Snippet: Single-cell RNA sequencing analysis revealing ECMSig expression across cell types and identification of prognostically relevant cell states in GBM (A) UMAP visualization of major cell types identified in GBM scRNA-seq data. (B) Dot plot showing the scaled average expression (color intensity) and percentage of cells expressing (dot size) canonical marker genes for each major cell type. (C) Dot plot showing the scaled average expression and percentage of cells expressing the seven ECMSig genes across major cell types. (D) UMAP plots showing the expression levels of individual ECMSig genes and overall ECMSig score across all cells. (E–G) UMAP plots illustrating Scissor-identified prognostically unfavorable (Scissor_Pos, red dashed circle) and favorable (Scissor_Neg, blue dashed circle; Scissor_Others, gray) cell subpopulations within (E) tumor cells, (F) myeloid cells, and (G) endothelial cells. (H–K) Violin plots comparing ECMSig scores among tumor cells grouped by Scissor status (H) and tumor type (I), and myeloid cells (J) or endothelial cells (K) grouped by Scissor status. ∗∗∗∗ p < 0.0001. Wilcoxon signed-rank test. (L) Dot plot showing differentially expressed marker genes between myeloid Scissor_Pos and other myeloid cells. Dot size indicates the fraction of cells in the group expressing the gene; color indicates average expression level.

    Article Snippet: The single-cell transcriptomic sequencing dataset utilizing technology from the 10X Genomics platform was available under the accession number GEO: GSE182109 at the Gene Expression Omnibus (GEO) repository.

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    Spatial transcriptomic analysis revealing co-localization of ECMSig, hypoxia, Scissor-Positive cells, and pericytes in GBM (A) Spatial feature plots for four GBM samples. Each row represents a sample. Columns show spatial heatmaps of: ECMSig score, hypoxia signature score, tumor Scissor_Pos signature score, myeloid Scissor_Pos signature score, endothelial Scissor Pos signature score, and pericyte marker signature score. Color scale indicates scaled expression or score (low to high). Each dot represents a spatial barcoded spot.

    Journal: iScience

    Article Title: Multi-omics profiling-derived signature links cellular ecosystem to glioblastoma prognosis

    doi: 10.1016/j.isci.2026.115982

    Figure Lengend Snippet: Spatial transcriptomic analysis revealing co-localization of ECMSig, hypoxia, Scissor-Positive cells, and pericytes in GBM (A) Spatial feature plots for four GBM samples. Each row represents a sample. Columns show spatial heatmaps of: ECMSig score, hypoxia signature score, tumor Scissor_Pos signature score, myeloid Scissor_Pos signature score, endothelial Scissor Pos signature score, and pericyte marker signature score. Color scale indicates scaled expression or score (low to high). Each dot represents a spatial barcoded spot.

    Article Snippet: The single-cell transcriptomic sequencing dataset utilizing technology from the 10X Genomics platform was available under the accession number GEO: GSE182109 at the Gene Expression Omnibus (GEO) repository.

    Techniques: Marker, Expressing