Review



nebnext ultratm ii dna library prep kit for illumina new england biolabs cat  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 99
      Buy from Supplier

    Structured Review

    New England Biolabs nebnext ultratm ii dna library prep kit for illumina new england biolabs cat
    Nebnext Ultratm Ii Dna Library Prep Kit For Illumina New England Biolabs Cat, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 12134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+transcriptomic+sequencing+dataset/NEBNext+Ultra+II+DNA+Library+Prep+Kit+for+Illumina/pm40614739-319-89-98
    Average 99 stars, based on 12134 article reviews
    nebnext ultratm ii dna library prep kit for illumina new england biolabs cat - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Sequencing:

    Article Title: Gut virome remodeling after fecal microbiota transplantation is associated with virus-bacteria co-variation and metabolic response in obesity and type 2 diabetes.
    Article Snippet: .. Sequencing libraries were prepared from 1 μg DNA per sample using the NEBNext Ultra DNA Library Prep Kit (Illumina) and sequenced on an Illumina NovaSeq 6000 platform with pairedend 150 bp reads, generating a median of 49.8 million reads per sample. ..

    Article Title: A comprehensive HA-tagged PRC1 toolkit enables standardized chromatin profiling and proteomic analysis in Drosophila melanogaster.
    Article Snippet: Following decrosslinking, DNA was purified using 220 MicroChIP DiaPure columns (Diagenode). .. DNA libraries for sequencing were prepared using 221 the NEBNext Ultra II DNA Library Prep Kit for Illumina. .. Paired-end sequencing (sequencing 222 (150 bp; approximately 2 Gb per sample) was performed by Novogene 223 (https://en.novogene.com/).

    Article Title: Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota
    Article Snippet: The product was quantified using the Quant‐iT PicoGreen dsDNA Assay Kit. .. Sequencing libraries were constructed using the NEB Next Ultra II DNA Library Prep Kit (New England Biolabs, Inc., USA). .. After passing quality control, the library was sequenced at both ends on the Illumina NovaSeq 6000 platform (Illumina, Inc., USA).

    Article Title: Identical consensus genomes of bovine coronavirus in respiratory and enteric specimens from a single cow and sequence variation after cell culture passage.
    Article Snippet: 1 Laboratory of Veterinary Microbiology, Joint Department of Veterinary Medicine, Gifu University, Yanagido, Gifu 501-1193, Japan 2 Joint Graduate School of Veterinary Sciences, Gifu University, Yanagido, Gifu 501-1193, Japan 3 Education and Research Center for Food Animal Health, Gifu University, Yanagido, Gifu 501-1193, Japan 4 Center for One Medicine Innovative Translational Research, Gifu University, Yanagido, Gifu 501-1193, Japan 5 Gifu Prefectural Chuo Livestock Hygiene Service Center, Yanagido, Gifu 501-1193, Japan 6 Joint Graduate School of Veterinary Sciences, Gifu University, 1-1, Yanagido, Gifu 501-1193, Japan Abstract Bovine coronavirus (BCoV) causes respiratory and enteric diseases in cattle, but the relationship between viral genetic variation and anatomical distribution remains unclear.. We compared BCoV genomes detected in a tracheal swab and diarrheic intestinal content from the same adult cow.. The two clinical specimens had identical 31,015-nt consensus genomes.

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Construct:

    Article Title: Electroacupuncture Alleviates Comorbid Depression‐Like Behaviors and Dyspepsia‐Like Symptoms in Rats Accompanied by Alterations in Hippocampal Neuroinflammation and Gut Microbiota
    Article Snippet: The product was quantified using the Quant‐iT PicoGreen dsDNA Assay Kit. .. Sequencing libraries were constructed using the NEB Next Ultra II DNA Library Prep Kit (New England Biolabs, Inc., USA). .. After passing quality control, the library was sequenced at both ends on the Illumina NovaSeq 6000 platform (Illumina, Inc., USA).

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    DNA Library Preparation:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Amplification:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Sonication:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Purification:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.

    Single Cell:

    Article Title: Clonal circulating tumor cell mobilization following chimeric antigen receptor T-cell therapy in metastatic castration-resistant prostate cancer
    Article Snippet: Afterward, DNA was purified with a QIAquick PCR Purification Kit (Qiagen, Cat#. 28106) with elution in 50 μL of Tris-EDTA buffer. .. Libraries for sequencing were constructed using the NEBNext Ultra DNA Library Preparation Kit from Illumina (New England Biolabs, Cat#. E7370L), which amplified and sonicated 50 μg of the previously purified single-cell DNA. .. The library concentration and anticipated size distribution of 300–500 bp were quantified via Qubit (Thermo Fisher) and the Agilent 2100 Bioanalyzer (High-Sensitivity DNA Assay and Kit, Agilent Technologies, Cat#. 5067–4626), respectively.



    Similar Products

    cell transcriptomic sequencing dataset  (10X Genomics)
    86
    10X Genomics cell transcriptomic sequencing dataset
    <t>Transcriptomic</t> and TME characteristics associated with ECMSig in TCGA-GBM cohort (A) Volcano plot showing DEGs between ECMSig-high and ECMSig-low groups. Red dots: upregulated in high-risk; blue dots: upregulated in low-risk. Benjamini-Hochberg adjusted. (B) Gene set enrichment analysis (GSEA) plots showing enrichment of hallmark pathways. Pathways enriched in ECMSig-high and ECMSig-low groups are shown with their running enrichment scores (ESs) and ranked gene lists. Benjamini-Hochberg adjusted. (C) Heatmap showing the activity scores of selected oncogenic and tumor-related signaling pathways (rows) across TCGA-GBM samples (columns), annotated by ECMSig group and ECMSig score. Red indicates high activity, blue indicates low activity. ∗ p < 0.05. Wilcoxon signed-rank test. (D) Heatmap depicting the estimated infiltration levels of various immune and stromal cell types (rows) in TCGA-GBM samples (columns), stratified by ECMSig group and score. Red indicates high infiltration, blue indicates low infiltration. Cells significantly highly infiltrated in ECMSig-high are labeled in red, and those high in ECMSig-low group are in blue. ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001. Wilcoxon signed-rank test. Benjamini-Hochberg adjusted. (E and F) Scatterplots showing the spearman correlation between ECMSig score and (E) Macrophage_XCELL infiltration score and (F) immune_score_XCELL. The blue line represents the linear regression fit with 95% confidence interval bands. Spearman correlation test.
    Cell Transcriptomic Sequencing Dataset, supplied by 10X Genomics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+transcriptomic+sequencing+dataset/cell+copy+genomic+number+profiling+single+technologies/pmc13197639-319-1-9
    Average 86 stars, based on 1 article reviews
    cell transcriptomic sequencing dataset - by Bioz Stars, 2026-11
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Transcriptomic and TME characteristics associated with ECMSig in TCGA-GBM cohort (A) Volcano plot showing DEGs between ECMSig-high and ECMSig-low groups. Red dots: upregulated in high-risk; blue dots: upregulated in low-risk. Benjamini-Hochberg adjusted. (B) Gene set enrichment analysis (GSEA) plots showing enrichment of hallmark pathways. Pathways enriched in ECMSig-high and ECMSig-low groups are shown with their running enrichment scores (ESs) and ranked gene lists. Benjamini-Hochberg adjusted. (C) Heatmap showing the activity scores of selected oncogenic and tumor-related signaling pathways (rows) across TCGA-GBM samples (columns), annotated by ECMSig group and ECMSig score. Red indicates high activity, blue indicates low activity. ∗ p < 0.05. Wilcoxon signed-rank test. (D) Heatmap depicting the estimated infiltration levels of various immune and stromal cell types (rows) in TCGA-GBM samples (columns), stratified by ECMSig group and score. Red indicates high infiltration, blue indicates low infiltration. Cells significantly highly infiltrated in ECMSig-high are labeled in red, and those high in ECMSig-low group are in blue. ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001. Wilcoxon signed-rank test. Benjamini-Hochberg adjusted. (E and F) Scatterplots showing the spearman correlation between ECMSig score and (E) Macrophage_XCELL infiltration score and (F) immune_score_XCELL. The blue line represents the linear regression fit with 95% confidence interval bands. Spearman correlation test.

    Journal: iScience

    Article Title: Multi-omics profiling-derived signature links cellular ecosystem to glioblastoma prognosis

    doi: 10.1016/j.isci.2026.115982

    Figure Lengend Snippet: Transcriptomic and TME characteristics associated with ECMSig in TCGA-GBM cohort (A) Volcano plot showing DEGs between ECMSig-high and ECMSig-low groups. Red dots: upregulated in high-risk; blue dots: upregulated in low-risk. Benjamini-Hochberg adjusted. (B) Gene set enrichment analysis (GSEA) plots showing enrichment of hallmark pathways. Pathways enriched in ECMSig-high and ECMSig-low groups are shown with their running enrichment scores (ESs) and ranked gene lists. Benjamini-Hochberg adjusted. (C) Heatmap showing the activity scores of selected oncogenic and tumor-related signaling pathways (rows) across TCGA-GBM samples (columns), annotated by ECMSig group and ECMSig score. Red indicates high activity, blue indicates low activity. ∗ p < 0.05. Wilcoxon signed-rank test. (D) Heatmap depicting the estimated infiltration levels of various immune and stromal cell types (rows) in TCGA-GBM samples (columns), stratified by ECMSig group and score. Red indicates high infiltration, blue indicates low infiltration. Cells significantly highly infiltrated in ECMSig-high are labeled in red, and those high in ECMSig-low group are in blue. ∗q < 0.05, ∗∗q < 0.01, ∗∗∗q < 0.001. Wilcoxon signed-rank test. Benjamini-Hochberg adjusted. (E and F) Scatterplots showing the spearman correlation between ECMSig score and (E) Macrophage_XCELL infiltration score and (F) immune_score_XCELL. The blue line represents the linear regression fit with 95% confidence interval bands. Spearman correlation test.

    Article Snippet: The single-cell transcriptomic sequencing dataset utilizing technology from the 10X Genomics platform was available under the accession number GEO: GSE182109 at the Gene Expression Omnibus (GEO) repository.

    Techniques: Activity Assay, Protein-Protein interactions, Labeling

    Single-cell RNA sequencing analysis revealing ECMSig expression across cell types and identification of prognostically relevant cell states in GBM (A) UMAP visualization of major cell types identified in GBM scRNA-seq data. (B) Dot plot showing the scaled average expression (color intensity) and percentage of cells expressing (dot size) canonical marker genes for each major cell type. (C) Dot plot showing the scaled average expression and percentage of cells expressing the seven ECMSig genes across major cell types. (D) UMAP plots showing the expression levels of individual ECMSig genes and overall ECMSig score across all cells. (E–G) UMAP plots illustrating Scissor-identified prognostically unfavorable (Scissor_Pos, red dashed circle) and favorable (Scissor_Neg, blue dashed circle; Scissor_Others, gray) cell subpopulations within (E) tumor cells, (F) myeloid cells, and (G) endothelial cells. (H–K) Violin plots comparing ECMSig scores among tumor cells grouped by Scissor status (H) and tumor type (I), and myeloid cells (J) or endothelial cells (K) grouped by Scissor status. ∗∗∗∗ p < 0.0001. Wilcoxon signed-rank test. (L) Dot plot showing differentially expressed marker genes between myeloid Scissor_Pos and other myeloid cells. Dot size indicates the fraction of cells in the group expressing the gene; color indicates average expression level.

    Journal: iScience

    Article Title: Multi-omics profiling-derived signature links cellular ecosystem to glioblastoma prognosis

    doi: 10.1016/j.isci.2026.115982

    Figure Lengend Snippet: Single-cell RNA sequencing analysis revealing ECMSig expression across cell types and identification of prognostically relevant cell states in GBM (A) UMAP visualization of major cell types identified in GBM scRNA-seq data. (B) Dot plot showing the scaled average expression (color intensity) and percentage of cells expressing (dot size) canonical marker genes for each major cell type. (C) Dot plot showing the scaled average expression and percentage of cells expressing the seven ECMSig genes across major cell types. (D) UMAP plots showing the expression levels of individual ECMSig genes and overall ECMSig score across all cells. (E–G) UMAP plots illustrating Scissor-identified prognostically unfavorable (Scissor_Pos, red dashed circle) and favorable (Scissor_Neg, blue dashed circle; Scissor_Others, gray) cell subpopulations within (E) tumor cells, (F) myeloid cells, and (G) endothelial cells. (H–K) Violin plots comparing ECMSig scores among tumor cells grouped by Scissor status (H) and tumor type (I), and myeloid cells (J) or endothelial cells (K) grouped by Scissor status. ∗∗∗∗ p < 0.0001. Wilcoxon signed-rank test. (L) Dot plot showing differentially expressed marker genes between myeloid Scissor_Pos and other myeloid cells. Dot size indicates the fraction of cells in the group expressing the gene; color indicates average expression level.

    Article Snippet: The single-cell transcriptomic sequencing dataset utilizing technology from the 10X Genomics platform was available under the accession number GEO: GSE182109 at the Gene Expression Omnibus (GEO) repository.

    Techniques: Single Cell, RNA Sequencing, Expressing, Marker

    Spatial transcriptomic analysis revealing co-localization of ECMSig, hypoxia, Scissor-Positive cells, and pericytes in GBM (A) Spatial feature plots for four GBM samples. Each row represents a sample. Columns show spatial heatmaps of: ECMSig score, hypoxia signature score, tumor Scissor_Pos signature score, myeloid Scissor_Pos signature score, endothelial Scissor Pos signature score, and pericyte marker signature score. Color scale indicates scaled expression or score (low to high). Each dot represents a spatial barcoded spot.

    Journal: iScience

    Article Title: Multi-omics profiling-derived signature links cellular ecosystem to glioblastoma prognosis

    doi: 10.1016/j.isci.2026.115982

    Figure Lengend Snippet: Spatial transcriptomic analysis revealing co-localization of ECMSig, hypoxia, Scissor-Positive cells, and pericytes in GBM (A) Spatial feature plots for four GBM samples. Each row represents a sample. Columns show spatial heatmaps of: ECMSig score, hypoxia signature score, tumor Scissor_Pos signature score, myeloid Scissor_Pos signature score, endothelial Scissor Pos signature score, and pericyte marker signature score. Color scale indicates scaled expression or score (low to high). Each dot represents a spatial barcoded spot.

    Article Snippet: The single-cell transcriptomic sequencing dataset utilizing technology from the 10X Genomics platform was available under the accession number GEO: GSE182109 at the Gene Expression Omnibus (GEO) repository.

    Techniques: Marker, Expressing