p16 clone jc8 (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology p16 clone jc8

( A ) Morphology of ASCs infected with shCntrl and shDIRAS3 was documented using light microscope at 40x magnification. (B and C) ASCs infected with either shDIRAS3 or shControl (shCntrl) expressing lentiviruses were fixed and stained for SA-β-GAL. Percentage of SA-β-GAL positive cells was calculated by scanning 5 low-power magnification fields (n=3). (D) ASCs were transduced by indicated lentiviruses with increasing MOI and cell lysates immunoblotted using phospho-Ser-139 Gamma H2A.X antibody. β-Actin served as a loading control. DIRAS3 was KD in ASCs using lentiviruses expressing specific shRNA at increasing MOI. Cell lysates were immunoblotted with the specific antibodies to investigate accumulation of senescent associated proteins <t>p16</t> <t>INK4A</t> , p21 CIP1 , p53 phosphorylated p53 (S15), Rb and pRb (S807/811). β-Actin served as a loading control. Fold changes in densitometric band intensities for phosphorylated proteins normalized to un-phosphorylated total proteins, acquired by image J were compared. Band intensity of shCntrl was taken as 1. Western blot shown is from replicate from one donor with similar protein expression pattern was observed with 2 different donors. All error bars represents the means ± SEM. p values * = p<0.05, **= p<0.001 and *** = p<.0001.

P16 Clone Jc8, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1629 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+transcriptome+sequencing+analysis/p16/pmc05391236-156-13-16
Average 96 stars, based on 1629 article reviews

p16 clone jc8 - by Bioz Stars, 2026-11

96/100 stars

Images

1) Product Images from "Silencing of the small GTPase DIRAS3 induces cellular senescence in human white adipose stromal/progenitor cells"

Article Title: Silencing of the small GTPase DIRAS3 induces cellular senescence in human white adipose stromal/progenitor cells

Journal: Aging (Albany NY)

doi: 10.18632/aging.101197

Figure Legend Snippet: ( A ) Morphology of ASCs infected with shCntrl and shDIRAS3 was documented using light microscope at 40x magnification. (B and C) ASCs infected with either shDIRAS3 or shControl (shCntrl) expressing lentiviruses were fixed and stained for SA-β-GAL. Percentage of SA-β-GAL positive cells was calculated by scanning 5 low-power magnification fields (n=3). (D) ASCs were transduced by indicated lentiviruses with increasing MOI and cell lysates immunoblotted using phospho-Ser-139 Gamma H2A.X antibody. β-Actin served as a loading control. DIRAS3 was KD in ASCs using lentiviruses expressing specific shRNA at increasing MOI. Cell lysates were immunoblotted with the specific antibodies to investigate accumulation of senescent associated proteins p16 INK4A , p21 CIP1 , p53 phosphorylated p53 (S15), Rb and pRb (S807/811). β-Actin served as a loading control. Fold changes in densitometric band intensities for phosphorylated proteins normalized to un-phosphorylated total proteins, acquired by image J were compared. Band intensity of shCntrl was taken as 1. Western blot shown is from replicate from one donor with similar protein expression pattern was observed with 2 different donors. All error bars represents the means ± SEM. p values * = p<0.05, **= p<0.001 and *** = p<.0001.

Techniques Used: Infection, Light Microscopy, Expressing, Staining, Control, shRNA, Western Blot

( A ) (Upper panel) Human shDIRAS3 ASCs (shDIRAS3 hASCs) and shCntrl hASCs were xenotransplanted into posterior sWAT of SCID mice. Injection sites of hASCs were histologically identified and marked by H&E staining. (Lower panel) Senescent DIRAS3 KD hASCs were detected by immunohistochemical staining using anti p16 INK4A antibodies. Region of Interest (ROI) is shown in higher magnification. (B) Number of p16 INK4A positive hASCs per section were counted and plotted (n = 5 per group). (C) (Left panel) Cell lysates from control ASCs and DIRAS3 KD ASCs cultured with and without 20 nM rapamycin were blotted for p16 INK4A , Rb, pRb (S807/811), P70S6K and pP70S6K (T389) using specific antibodies. β-Actin served as loading control. Note, rapamycin was added 2 days after virus infection. (Right panels) Fold changes in densitometric band intensities presented as Arbitrary Units (AU) for phosphorylated proteins normalized to un-phosphorylated total proteins, acquired by image J were compared. Band intensity of shCntrl was taken as 1 (n=2). All error bars represents the means ± SEM. p values * = p<0.05, **= p<0.001 and *** = p<.0001.

Figure Legend Snippet: ( A ) (Upper panel) Human shDIRAS3 ASCs (shDIRAS3 hASCs) and shCntrl hASCs were xenotransplanted into posterior sWAT of SCID mice. Injection sites of hASCs were histologically identified and marked by H&E staining. (Lower panel) Senescent DIRAS3 KD hASCs were detected by immunohistochemical staining using anti p16 INK4A antibodies. Region of Interest (ROI) is shown in higher magnification. (B) Number of p16 INK4A positive hASCs per section were counted and plotted (n = 5 per group). (C) (Left panel) Cell lysates from control ASCs and DIRAS3 KD ASCs cultured with and without 20 nM rapamycin were blotted for p16 INK4A , Rb, pRb (S807/811), P70S6K and pP70S6K (T389) using specific antibodies. β-Actin served as loading control. Note, rapamycin was added 2 days after virus infection. (Right panels) Fold changes in densitometric band intensities presented as Arbitrary Units (AU) for phosphorylated proteins normalized to un-phosphorylated total proteins, acquired by image J were compared. Band intensity of shCntrl was taken as 1 (n=2). All error bars represents the means ± SEM. p values * = p<0.05, **= p<0.001 and *** = p<.0001.

Techniques Used: Injection, Staining, Immunohistochemical staining, Control, Cell Culture, Virus, Infection

( A ) (Left panel) Adipogenic differentiation of ASCs was estimated by staining the cells with Oil-Red-O stain at day 9 post induction of differentiation. Non-induced ASC controls are shown. (Right panel) Quantification of Oil-Red-O stained area using image J is shown (n=3). (B) Expression of PPAR γ 2, FABP4 and Perilipin mRNA was estimated at day 3 and day 9 post induction of adipogenesis. Expression at day 0 before induction was taken as 1 and fold increase was calculated. Values are normalized to β Actin. (C) Perilipin and p16 INK4A protein levels were analysed in DIRAS3 KD and control ASCs at day 9 post induction of adipogenesis by western blotting. β-Actin protein served as input control. (D and E) Densitometric evaluation of western blots bands from figure C. Fold changes in densitometric band intensities of perilipin (D) and p16 INK4A (E) normalized to β-Actin protein, acquired by image J were plotted. (F) p16 INK4A mRNA expression was analysed in DIRAS3 KD (red) and control ASCs (black) at day 3 and 9 post adipogenesis induction by q-RT_PCR analysis. All error bars represents the means ± SEM. p values * = p<0.05, **= p<0.001 and *** = p<.0001.

Figure Legend Snippet: ( A ) (Left panel) Adipogenic differentiation of ASCs was estimated by staining the cells with Oil-Red-O stain at day 9 post induction of differentiation. Non-induced ASC controls are shown. (Right panel) Quantification of Oil-Red-O stained area using image J is shown (n=3). (B) Expression of PPAR γ 2, FABP4 and Perilipin mRNA was estimated at day 3 and day 9 post induction of adipogenesis. Expression at day 0 before induction was taken as 1 and fold increase was calculated. Values are normalized to β Actin. (C) Perilipin and p16 INK4A protein levels were analysed in DIRAS3 KD and control ASCs at day 9 post induction of adipogenesis by western blotting. β-Actin protein served as input control. (D and E) Densitometric evaluation of western blots bands from figure C. Fold changes in densitometric band intensities of perilipin (D) and p16 INK4A (E) normalized to β-Actin protein, acquired by image J were plotted. (F) p16 INK4A mRNA expression was analysed in DIRAS3 KD (red) and control ASCs (black) at day 3 and 9 post adipogenesis induction by q-RT_PCR analysis. All error bars represents the means ± SEM. p values * = p<0.05, **= p<0.001 and *** = p<.0001.

Techniques Used: Staining, Expressing, Control, Western Blot, Reverse Transcription Polymerase Chain Reaction

Figure Legend Snippet: The primer sequences used the present study are indicated

Techniques Used: Sequencing

Staining:

Article Title: A multimodal ROS logic-gated therapeutic platform disrupts the vicious cycle of senescence to promote aged bone defect repair
Article Snippet: .. Tissue sections were stained for γ-H2A.X (Servicebio, GB111841 , 1:500), p62 (Affinity, AF5384, 1:500), p16 (Santa Cruz, sc-81156, 1:200), p21 (Affinity, AF6290, 1:300), Cyclin D1 (Proteintech, 26939-1-AP, 1:200), MMP-2 (Servicebio, GB11130, 1:500), IL-6 (Servicebio, GB11117, 1:200), and TNF-α (Servicebio, GB115702 , 1:200) following standard protocols. .. Fluorescent images were acquired using a panoramic digital slide scanner (VS200, Olympus, Japan).

other:

Article Title: Exploring the dual role of extracellular vesicles in coagulation and immune modulation in glioblastoma.
Article Snippet: ◂ Scientific Reports | (2026) 16:9534 15| https://doi.org/10.1038/s41598-026-42867-4 Red (Stock: 5 mM, Invitrogen, Waltham, Massachusetts, United States, 1:1250) and Calcein-AM (4 μM, 30 min, 37 °C).

Incubation:

Article Title: Alteration in vitamin D catabolic enzyme 24-hydroxylase in aging renal tubules affect tubular transport function
Article Snippet: .. The sections were incubated overnight at 4 °C with primary antibodies against the following target proteins: 24- OHase (Proteintech; Cat#21582-1-AP; 1:50), 1α-OHase (Abcam; Cat# ab206655; 1:100), VDR (CST; Cat# 12550; 1:200), NPT2a (Novus; Cat# NBP2-13328; 1:50), and SGLT2 (Proteintech; Cat# 24654-1-AP; 1:200), p16 (Santa Cruz; Cat# sc-56330;1:100), p53 (Santa; Cat# SC-6243; 1:100), p21 (Abcam; Cat# ..

Phospho-proteomics:

Article Title: The novel synbiotic (Lacticaseibacillus rhamnosus NKU FL1-11 and galacto-oligosaccharides) alleviates HCA IQ-triggered aging by reprogramming gut microbial metabolism to coordinate the enterohepatic β-glucuronidase activity.
Article Snippet: 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ), a nitrogen-containing heterocyclic amine, mainly enters the human body through thermally processed foods and is recognized as a dietary organic pollutant.. Although current toxicological studies have focused on the direct health effects of IQ, its pro-gerontic potential remains unknown.. In this study, we evaluated the efficacy of a novel synbiotic composed of Lacticaseibacillus rhamnosus NKU FL1–11 and galacto-oligosaccharides against IQ-triggered aging in zebrafish.

Cell-Signaling:

Article Title: Overcoming acquired doxorubicin resistance of ovarian carcinoma cells by verapamil-mediated promotion of DNA damage-driven cytotoxicity
Article Snippet: Chemicals were obtained from the following providers: Entinostat (MS-275) was obtained from Selleck Chemicals, Doxo from STADA Consumer Health & STADAPHARM GmbH, etoposide, Ehop16, prexasertib (AZD-7762) and dexrazoxane were from MilliporeSigma, cisplatin from Accord Healthcare GmbH, olaparib from APeXBIO Technology LLC, niraparib from MedChemExpress, EHT1864 was purchased from Tocris Bioscience, rabusertib (LY2603618) and ricolinostat (ACY-1215) from MedChemExpress and verapamil (Ver) from Thermo Fisher Scientific, Inc. .. The following primary antibodies were used: Copper transporting ATPase (ATP7A), extracellular regulated kinase 2 (ERK2), phosphorylated (p)-histone H3 (Ser10) from Thermo Fisher Scientific Inc., cleaved caspase-7 (Asp198), p-Chk1 (Ser 345), cyclin B1, galactosidase β (E2U2I), GAPDH (14C10), MDR1/ABCB1 (D3H1Q), p-P53 (S15), PARP, TopBP1(D8G4L), topoisomerase IIa (D10G9), 53BP1 and Ki67 were from Cell Signaling Technology Inc., pChk2 (T68) [Y171], copper uptake protein 1 (CTR1/SLC31A1) [EPR7936] and Rad51 from Abcam, γH2AX (Ser 139) clone JBW301, p-KAP-1 (S824) and p-RPA32 (S4/S8) from Bethyl Laboratories Inc., organic cation transporter-2 (OCT2) from Biozol Diagnostics Vertrieb GmbH, p16 (F-12) and p21 (C-19) from Santa Cruz Biotechnology, Inc. As secondary antibodies, horseradish peroxidase-conjugated secondary antibodies goat anti-mouse IgG and mouse anti-rabbit IgG were used (Rockland Immunochemicals Inc.). .. Parental A2780 ovarian carcinoma cells (A2780) as well as a doxorubicin resistant variant (A2780ADR) were from the European Collection of Authenticated Cell Cultures and were cultured in RPMI-1640 medium (MilliporeSigma) containing 10% fetal calf serum, 1% glutamine and 1% Pen/Strep at 37°C in a humidified atmosphere containing 5% CO 2 .

Blocking Assay:

Article Title: 8-Oxoguanine modifications in seed regions of miRNAs regulating P16 promote aging by reshaping gene regulatory networks.
Article Snippet: The mechanisms underlying upregulation of P16, a key marker of cellular senescence, during the senescence process remain unclear.. We observed that miR-134-5p and miR-3118 effectively inhibited P16 expression and delayed cellular senescence in human dermal fibroblast cells.. The reactive oxygen species-induced 8-oxo-guanine (o8G) modification of miR-134-5p and miR-3118 disrupted their regulatory interactions with the primary target P16, promoting cellular senescence, as indicated by increased β-galactosidase activity, enhanced G1 phase arrest, reduced expression of anti-aging proteins, elevated secretion of senescence-associated secretory phenotype factors, and impaired autophagy.

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