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desmin  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology desmin
    Sequence Information of Primers Used for Reverse Transcriptase–Polymerase Chain Reaction and Their Expected Product Size
    Desmin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 457 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+transcriptome+sequencing+analysis/Desmin+Antibody/pmc02952115-102-35-37
    Average 94 stars, based on 457 article reviews
    desmin - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Bladder Cells: Potential for Urological Tissue Engineering"

    Article Title: Differentiation of Human Bone Marrow Mesenchymal Stem Cells into Bladder Cells: Potential for Urological Tissue Engineering

    Journal: Tissue Engineering. Part A

    doi: 10.1089/ten.tea.2009.0625

    Sequence Information of Primers Used for Reverse Transcriptase–Polymerase Chain Reaction and Their Expected Product Size
    Figure Legend Snippet: Sequence Information of Primers Used for Reverse Transcriptase–Polymerase Chain Reaction and Their Expected Product Size

    Techniques Used: Sequencing

    Expression of muscle and urothelial lineage-specific transcripts in differentiated human BMSCs by reverse transcriptase–polymerase chain reaction on day 14. (A) Smooth muscle-specific primers (α-SMA, desmin, calponin, MHC) used on RNA extracted from human BMSCs (p4) induced with human bladder SMC-derived conditional medium for 14 days. Lane 1, fresh smooth muscle tissue; lane 2, cultured SMCs as positive controls; lane 3, cultured urothelium as negative control; lane 4, noninduced BMSCs as control; lane 5, induced BMSCs by coculture with SMCs; lane 6, induced BMSCs by SMC-CM; lane 7, no template control. (B) Urothelial-specific primers (Up-Ia, CK-7/13) used on RNA samples from human BMSCs (p4) induced by coculture with urothelium for 7 days. Lane 1, cultured urothelium as positive; lane 2, noninduced BMSCs as control; lane 3, BMSCs cocultured with urothelium; lane 4, BMSCs cultured using urothelium-CM; lane 5, no template (H2O) control. GAPDH was used as the housekeeping gene for load control. Note: Threefold excess of the reaction mixture was loaded for all the primers in (A) compared with that in (B). Moreover, the bands for all the primers in (A) are overexposed, whereas the bands in (B) are under exposured. α-SMA, alpha smooth muscle actin; MHC, myosin heavy chain; Up-Ia, uroplakin-Ia; CK-7/13, cytokeratin-7/13; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.
    Figure Legend Snippet: Expression of muscle and urothelial lineage-specific transcripts in differentiated human BMSCs by reverse transcriptase–polymerase chain reaction on day 14. (A) Smooth muscle-specific primers (α-SMA, desmin, calponin, MHC) used on RNA extracted from human BMSCs (p4) induced with human bladder SMC-derived conditional medium for 14 days. Lane 1, fresh smooth muscle tissue; lane 2, cultured SMCs as positive controls; lane 3, cultured urothelium as negative control; lane 4, noninduced BMSCs as control; lane 5, induced BMSCs by coculture with SMCs; lane 6, induced BMSCs by SMC-CM; lane 7, no template control. (B) Urothelial-specific primers (Up-Ia, CK-7/13) used on RNA samples from human BMSCs (p4) induced by coculture with urothelium for 7 days. Lane 1, cultured urothelium as positive; lane 2, noninduced BMSCs as control; lane 3, BMSCs cocultured with urothelium; lane 4, BMSCs cultured using urothelium-CM; lane 5, no template (H2O) control. GAPDH was used as the housekeeping gene for load control. Note: Threefold excess of the reaction mixture was loaded for all the primers in (A) compared with that in (B). Moreover, the bands for all the primers in (A) are overexposed, whereas the bands in (B) are under exposured. α-SMA, alpha smooth muscle actin; MHC, myosin heavy chain; Up-Ia, uroplakin-Ia; CK-7/13, cytokeratin-7/13; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.

    Techniques Used: Expressing, Reverse Transcription, Polymerase Chain Reaction, Derivative Assay, Cell Culture, Negative Control, Control

    Myogenic differentiation of human BMSCs using SMC-derived CM. Human BMSCs (p4) were stained with α-SMA (a, e, i), calponin (b, f, j), desmin (c, g, k), and myosin (d, h, l) antibodies without induction as negative control (a–d) and with induction for 14 days (e–h). SMCs were also stained with the same antibodies as a positive control (i–l). Scale bar = 50 μM. Color images available online at www.liebertonline.com/ten.
    Figure Legend Snippet: Myogenic differentiation of human BMSCs using SMC-derived CM. Human BMSCs (p4) were stained with α-SMA (a, e, i), calponin (b, f, j), desmin (c, g, k), and myosin (d, h, l) antibodies without induction as negative control (a–d) and with induction for 14 days (e–h). SMCs were also stained with the same antibodies as a positive control (i–l). Scale bar = 50 μM. Color images available online at www.liebertonline.com/ten.

    Techniques Used: Derivative Assay, Staining, Negative Control, Positive Control



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