tie2 mural precursor cells tie2 antibodies (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology tie2 mural precursor cells tie2 antibodies

Fig. 5. <t>Tie2</t> expression in MPCs. (Top) Western-blot analysis (WB) of MPC protein extract immunoreacted for Tie2 demonstrated a 140 kDa band that migrated with the Tie2 band of the endothelial cell control (EC). RT-PCR conﬁrmed expression of Tie2 in MPCs at the mRNA level. Endothelial cells (EC) were used as positive control. No PCR bands were detected in the absence of reverse transcriptase (data not shown). Speciﬁcity of the PCR products was conﬁrmed by sequence analysis. (Bottom) Analysis of MPC protein extracts immunoprecipitated (IP) with an anti-Tie2 antibody and reacted with an anti-phosphotyrosine antibody (α-pY) showed phosphorylation of the MPC Tie2 receptor upon stimulation with both Ang-1 and Ang-2. Stripping and reprobing of the membrane with an anti-Tie2 antibody (α-Tie2) showed that equal amounts of Tie2 were present in each lane.

Tie2 Mural Precursor Cells Tie2 Antibodies, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 295 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+transcriptome+sequencing+analysis/pm12876214-76-24-30?v=Santa+Cruz+Biotechnology
Average 93 stars, based on 295 article reviews

tie2 mural precursor cells tie2 antibodies - by Bioz Stars, 2026-07

93/100 stars

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1) Product Images from "Rat aorta-derived mural precursor cells express the Tie2 receptor and respond directly to stimulation by angiopoietins."

Article Title: Rat aorta-derived mural precursor cells express the Tie2 receptor and respond directly to stimulation by angiopoietins.

Journal: Journal of cell science

doi: 10.1242/jcs.00629

Fig. 5. Tie2 expression in MPCs. (Top) Western-blot analysis (WB) of MPC protein extract immunoreacted for Tie2 demonstrated a 140 kDa band that migrated with the Tie2 band of the endothelial cell control (EC). RT-PCR conﬁrmed expression of Tie2 in MPCs at the mRNA level. Endothelial cells (EC) were used as positive control. No PCR bands were detected in the absence of reverse transcriptase (data not shown). Speciﬁcity of the PCR products was conﬁrmed by sequence analysis. (Bottom) Analysis of MPC protein extracts immunoprecipitated (IP) with an anti-Tie2 antibody and reacted with an anti-phosphotyrosine antibody (α-pY) showed phosphorylation of the MPC Tie2 receptor upon stimulation with both Ang-1 and Ang-2. Stripping and reprobing of the membrane with an anti-Tie2 antibody (α-Tie2) showed that equal amounts of Tie2 were present in each lane.

Figure Legend Snippet: Fig. 5. Tie2 expression in MPCs. (Top) Western-blot analysis (WB) of MPC protein extract immunoreacted for Tie2 demonstrated a 140 kDa band that migrated with the Tie2 band of the endothelial cell control (EC). RT-PCR conﬁrmed expression of Tie2 in MPCs at the mRNA level. Endothelial cells (EC) were used as positive control. No PCR bands were detected in the absence of reverse transcriptase (data not shown). Speciﬁcity of the PCR products was conﬁrmed by sequence analysis. (Bottom) Analysis of MPC protein extracts immunoprecipitated (IP) with an anti-Tie2 antibody and reacted with an anti-phosphotyrosine antibody (α-pY) showed phosphorylation of the MPC Tie2 receptor upon stimulation with both Ang-1 and Ang-2. Stripping and reprobing of the membrane with an anti-Tie2 antibody (α-Tie2) showed that equal amounts of Tie2 were present in each lane.

Techniques Used: Expressing, Western Blot, Control, Reverse Transcription Polymerase Chain Reaction, Positive Control, Reverse Transcription, Sequencing, Immunoprecipitation, Phospho-proteomics, Stripping Membranes, Membrane

Fig. 6. Photomicrographs of planar cultures of isolated MPCs (A-E) and endothelial cells (F), and of histologic sections of native rat aorta (G,H) immunostained for Tie2 by immunoperoxidase (A,B,F-H) or immunoﬂuorescence (C-E). Planar cultures: (A) shows a group of conﬂuent MPCs whereas (B) shows a single MPC at higher magniﬁcation. Immunoperoxidase staining of cultured cells demonstrated Tie2 in both MPCs (A,B) and control endothelial cells (F). The positive staining reaction for Tie2 was predominantly localized at the cell periphery (arrows). Confocal images of MPCs double stained for Tie2 (C, green ﬂuorescence) and α-SMA (D, red ﬂuorescence) showed coexpression of Tie2 and α-SMA in the same cells (E, green and red ﬂuorescence overlay). Cultured cells reacted with nonimmune IgG were negative (data not shown). Scale bars, 50 µM (A,C-E), 30 µM (B,F). Histological sections of native rat aorta: the intimal and subintimal layers of the aorta contain Tie2+ nonendothelial mesenchymal cells (G,H, arrows). Arrowheads highlight the endothelial lining of the aortic intima, which serves as a positive internal control. Scale bar, 100 µm.

Figure Legend Snippet: Fig. 6. Photomicrographs of planar cultures of isolated MPCs (A-E) and endothelial cells (F), and of histologic sections of native rat aorta (G,H) immunostained for Tie2 by immunoperoxidase (A,B,F-H) or immunoﬂuorescence (C-E). Planar cultures: (A) shows a group of conﬂuent MPCs whereas (B) shows a single MPC at higher magniﬁcation. Immunoperoxidase staining of cultured cells demonstrated Tie2 in both MPCs (A,B) and control endothelial cells (F). The positive staining reaction for Tie2 was predominantly localized at the cell periphery (arrows). Confocal images of MPCs double stained for Tie2 (C, green ﬂuorescence) and α-SMA (D, red ﬂuorescence) showed coexpression of Tie2 and α-SMA in the same cells (E, green and red ﬂuorescence overlay). Cultured cells reacted with nonimmune IgG were negative (data not shown). Scale bars, 50 µM (A,C-E), 30 µM (B,F). Histological sections of native rat aorta: the intimal and subintimal layers of the aorta contain Tie2+ nonendothelial mesenchymal cells (G,H, arrows). Arrowheads highlight the endothelial lining of the aortic intima, which serves as a positive internal control. Scale bar, 100 µm.

Techniques Used: Isolation, Immunoperoxidase Staining, Cell Culture, Control, Staining

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tie2 mural precursor cells tie2 antibodies (Santa Cruz Biotechnology)

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1) Product Images from "Rat aorta-derived mural precursor cells express the Tie2 receptor and respond directly to stimulation by angiopoietins."

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