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maxpar cell staining buffer  (fluidigm)


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    Structured Review

    fluidigm maxpar cell staining buffer
    Maxpar Cell Staining Buffer, supplied by fluidigm, used in various techniques. Bioz Stars score: 96/100, based on 713 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+staining+buffer/Maxpar+Cell+Staining+Buffer/pmc13043783-80-10-16
    Average 96 stars, based on 713 article reviews
    maxpar cell staining buffer - by Bioz Stars, 2026-09
    96/100 stars

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    Incubation:

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: .. Cells were incubated with 100μL of 1μM Cell-ID rhodium (Standard Biotools, 201103A) for 30 minutes at room temperature and then washed with 3ml of Maxpar Cell staining buffer (Standard Biotools, 201068). .. Cells were blocked using 10μL of human TruStain FcX (Biolegend, 422301) for 10 minutes at room temperature.

    Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study.
    Article Snippet: .. 1–3 million cells of each cell line were resuspended in Maxpar® Cell Staining Buffer (CSB, Standard BioTools) and then were incubated with 4 μg/mL of [175Lu]Lu-DOTA-cAC10 at room temperature for 30 min. ..

    Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study
    Article Snippet: .. 1–3 million cells of each cell line were resuspended in Maxpar ® Cell Staining Buffer (CSB, Standard BioTools) and then were incubated with 4 μg/mL of [ 175 Lu]Lu-DOTA-cAC10 at room temperature for 30 min. ..

    Staining:

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: .. Cells were incubated with 100μL of 1μM Cell-ID rhodium (Standard Biotools, 201103A) for 30 minutes at room temperature and then washed with 3ml of Maxpar Cell staining buffer (Standard Biotools, 201068). .. Cells were blocked using 10μL of human TruStain FcX (Biolegend, 422301) for 10 minutes at room temperature.

    Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study.
    Article Snippet: .. 1–3 million cells of each cell line were resuspended in Maxpar® Cell Staining Buffer (CSB, Standard BioTools) and then were incubated with 4 μg/mL of [175Lu]Lu-DOTA-cAC10 at room temperature for 30 min. ..

    Article Title: Single-cell analysis of signalling and transcriptional responses to type I interferons.
    Article Snippet: .. After washing with 5 ml Maxpar PBS (Fluidigm), cells were fixed with 1 X Maxpar Fix I Buffer (Fluidigm) for 10 min at room temperature before being washed with 1.5 ml Maxpar Cell Staining Buffer (CSB, Fluidigm). ..

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: .. Cells were washed with 2ml Maxpar cell staining buffer and fixed in 1.6% formaldehyde, diluted from 16% methanol free formaldehyde (ThermoScientific, 28906) in Maxpar PBS (Standard Biotools, 201058), for 10 minutes at room temperature. .. Cells were pelleted, supernatant removed, and stained overnight at 4°C with 1ml of 125nM Cell-ID Intercalator Iridium (Standard Biotools, 201192A), in Maxpar Fix and Perm Solution (Standard Biotools, 201067).

    Article Title: Circulating pre-osteoclasts are primed for osteoclast fate and synovial tissue homing in psoriatic arthritis
    Article Snippet: Cells were then transferred to 1.5ml LoBind Eppendorf’s, with care being taken to collect residual cells. .. Cells were washed 1x in Maxpar cell staining buffer followed by 2x washes in Maxpar cell acquisition solution (Standard Biotools, 201240). .. Cells were counted and resuspended to a maximum of 1×10 6 cells per ml in the 1.5ml Eppendorf.

    Article Title: The use of mass cytometry (CyTOF) to evaluate the cellular uptake of stable radiopharmaceutical surrogates in single cells: a proof-of-concept study
    Article Snippet: .. 1–3 million cells of each cell line were resuspended in Maxpar ® Cell Staining Buffer (CSB, Standard BioTools) and then were incubated with 4 μg/mL of [ 175 Lu]Lu-DOTA-cAC10 at room temperature for 30 min. ..

    Article Title: Mass cytometry uncovers distinct blood myeloid phenotypes linked to clinical responses during gastric cancer chemoimmunotherapy
    Article Snippet: .. Initially, the cells were washed with MaxPar cell staining buffer (Standard BioTools Inc., South San Francisco, CA, USA). .. Cells were stained with 32 surface antibodies (Supplementary Table 1), including TIGIT antibody (Anti-Human TIGIT, MBSA43 clone), pan-keratin antibody (Anti-Human Pan-keratin, C11 clone), and a 30-marker antibody panel, Maxpar Direct Immune Profiling Panel (Standard BioTools Inc., South San Francisco, CA, USA).

    other:

    Article Title: Evaluation of nanobodies engineered with extra-cysteines for site-specific functionalization.
    Article Snippet: After washing, a freshly prepared 1.6% formaldehyde solution (Pierce, 16% Formaldehyde, 28906, Thermo Scientific) was added, and cells were incubated for 15 min at room temperature before centrifugation at 800 x g for 5 min. A barcoding strategy was applied using Cell-ID 20-Plex Pd Barcoding Kit (201060, Standard Biotools), allowing simultaneous analysis of up to 20 samples per experiment.



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