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5-brdu  (Bio-Techne corporation)


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    Bio-Techne corporation 5-brdu
    5 Brdu, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+separation+media+lympholyte+cl+5015/5-BrdU/custom%405015%4035915082
    Average 92 stars, based on 4 article reviews
    5-brdu - by Bioz Stars, 2026-11
    92/100 stars

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    Related Articles

    Synthesized:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Blocking Assay:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Modification:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Cell Culture:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Clinical Proteomics:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Filtration:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Centrifugation:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Membrane:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Liposomes:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Isolation:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Purification:

    Article Title: Microfluidic-Based Exosome Analysis for Liquid Biopsy.
    Article Snippet: B. Lin, J. Wang, L. Zhu, Y. Wu, Dr. H. Zhang, Prof. C. Yang The MOE Key Laboratory of Spectrochemical Analysis & Instrumentation The Key Laboratory of Chemical Biology of Fujian Province State Key Laboratory of Physical Chemistry of Solid Surfaces Collaborative Innovation Center of Chemistry for Energy Materials Department of Chemical Biology College of Chemistry and Chemical Engineering Xiamen University Xiamen 361005, China E-mail: cyyang@xmu.edu.cn Dr. Y. Lei, Dr. L. Wu, Prof. P. Zhang, Prof. C. Yang Institute of Molecular Medicine Renji Hospital Shanghai Jiao Tong University School of Medicine Shanghai 200127, China E-mail: pengzhang016@sjtu.edu.cn The ORCID identification number(s) for the author(s) of this article can be found under https://doi.org/10.1002/smtd.202001131.

    Incubation:

    Article Title: Developmental modeling of hepatogenesis using obese iPSCs-hepatocyte differentiation uncovers pathological features.
    Article Snippet: .. Differentiating cells at all four-time points were incubated with 0.4 μl/ml of 5-BrdU solution (20 μg/ml in H2O) (TOCRIS, Cat#5015) added directly to the growing media, followed by 60min of incubation at 37 °C. ..

    Article Title: MPTP induces neurodegeneration by modulating dopaminergic activity in catfish brain.
    Article Snippet: Tyrosine hydroxylase (Th) is an allosteric rate-limiting enzyme in catecholamine (CA) biosynthesis.. The CAs, dopamine (DA), norepinephrine (NE), and epinephrine are important neurotransmitters wherein DA contributes a key role in the central nervous system of vertebrates.. The present study evaluated DA and Th’s significance in DA-ergic activity and neurodegeneration upon 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) exposure in catfish.

    Article Title: O-GlcNAc transferase promotes glioblastoma by modulating genes responsible for cell survival, invasion, and inflammation.
    Article Snippet: .. Following treatments, BrdU (Tocris) was added to media at final concentration of 50 μM to the cells and incubated for 1 h. Cells were washed with PBS and fixed using 4% paraformaldehyde for 15 min at room temperature followed by permeabilization using 0.3% Triton X-100. ..

    Article Title: O-GlcNAc transferase promotes glioblastoma by modulating genes responsible for cell survival, invasion, and inflammation
    Article Snippet: .. Following treatments, BrdU (Tocris) was added to media at final concentration of 50 μM to the cells and incubated for 1 h. Cells were washed with PBS and fixed using 4% paraformaldehyde for 15 min at room temperature followed by permeabilization using 0.3% Triton X-100. ..

    Concentration Assay:

    Article Title: O-GlcNAc transferase promotes glioblastoma by modulating genes responsible for cell survival, invasion, and inflammation.
    Article Snippet: .. Following treatments, BrdU (Tocris) was added to media at final concentration of 50 μM to the cells and incubated for 1 h. Cells were washed with PBS and fixed using 4% paraformaldehyde for 15 min at room temperature followed by permeabilization using 0.3% Triton X-100. ..

    Article Title: O-GlcNAc transferase promotes glioblastoma by modulating genes responsible for cell survival, invasion, and inflammation
    Article Snippet: .. Following treatments, BrdU (Tocris) was added to media at final concentration of 50 μM to the cells and incubated for 1 h. Cells were washed with PBS and fixed using 4% paraformaldehyde for 15 min at room temperature followed by permeabilization using 0.3% Triton X-100. ..

    other:

    Article Title: Evaluation of prenatal calabash chalk geophagy on the developing brain of Wistar rats
    Article Snippet: Meanwhile, 5-bromo-2′-deoxyuridine (5-BrdU, 250 g, Tocris BioscienceTM 5015/50) was dissolved in distilled water and the pH adjusted to 7.35.

    Recombinant:

    Article Title: Bone marrow monocytes sustain NK cell-poiesis during non-alcoholic steatohepatitis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Ly76 (TER119) Sony Cat# 1181020; RRID:AB_3083430 Gr-1 (RB6-8C5) Sony Cat# 1142020;RRID:AB_3083431 CD3e (145-2C11) BD Biosciences Cat# 563565; RRID:AB_2738278 CD19 (MB19-1) BD Biosciences Cat# 553784; RRID:AB_395048 NK1.1 (PK136) BD Biosciences Cat# 564143; RRID:AB_2738617 IL-7Ra (A7R34) Sony Cat# 1275070; RRID:AB_3083432 CD8a (53-6.7) Sony Cat# 1103520;RRID:AB_3083433 CD4 (RM4-5) Sony Cat#1102540;RRID:AB_3083434 Thy1.2 (53-2.1) BD Biosciences Cat# 563008; RRID:AB_2665477 NKp46 (29A1.4) BD Biosciences Cat# 560756; RRID:AB_1727465 IFNg (XMG1.2) BD Biosciences Cat# 563773; RRID:AB_2738419 CD45.2 (104) BD Biosciences Cat# 563686; RRID:AB_2738375 CD49a (HMa1) BD Biosciences Cat# 564863; RRID:AB_2738987 CD49b (DX5) BD Biosciences Cat# 740133; RRID:AB_2739890 T-BET (4B10) Sony Cat# 3824020; RRID:AB_3083437 EOMES (Dan11mag) eBioscience Cat# 48-4875-82; RRID:AB_2574062 BCL-2 (BCL/10C4) Sony Cat# 3767520; RRID:AB_3083435 TNFa (MP6-XT22) Sony Cat# 3131620;RRID:AB_3083436 CD11b (M1/70) BD Biosciences Cat# 557960; RRID:AB_396960 CD11c (HL3) BD Biosciences Cat# 563735; RRID:AB_2738394 Granzyme B (GB12) Invitrogen Cat# MHGB04; RRID:AB_1500191 F4/80 (BM8) Sony Cat# 1215570; RRID:AB_3083438 MHCII (M5/114.15.2) BD Biosciences Cat# 563414; RRID:AB_2738191 CD226 (10E5) BD Biosciences Cat#565663; RRID:AB_2916365 CD94 (18d3) BD Biosciences Cat# 740951; RRID:AB_2740576 NKG2A (16A11) BD Biosciences Cat# 569527; RRID:AB_3083440 NKG2D (CX5) BD Biosciences Cat# 563694; RRID:AB_2722498 Ly6C (HK1.4) Sony Cat# 1240070; RRID:AB_3083349 Ly6G (1A8) BD Biosciences Cat# 551461; RRID:AB_394208 CD45 (30-F11) BD Biosciences Cat# 557659; RRID:AB_396774 CD122 (TM-b1) BD Biosciences Cat#553362; RRID:AB_394809 CD183 (CXCR3-173) BD Biosciences Cat#562266; RRID:AB_11153500 CD27 (LG.3A10) Sony Cat# 1221130 CD51 (RMV-7) eBioscience Cat#12-0512-82;RRID:AB_465704 CD44 (IM-7) BD Biosciences Cat#560569; RRID:AB_1727484 Ki67 (B56) BD Biosciences Cat#561165; RRID:AB_10611866 F4/80 (BM8) eBioscience Cat# 14-4801-82; RRID:AB_467558 CD25 BD Biosciences Cat#553866; RRID:AB_395101 anti-asialo GM1 antibody Invitrogen Cat# 16-6507-39; RRID:AB_10718540 Chemicals, peptides, and recombinant proteins 5-BrdU R&D systems Cat# 5015 Recombinant Mouse Osteopontin R&D systems Cat# 441-OP-050/CF Recombinant IL-15 BioLegend Cat# 566304 (Continued on next page) 18 Cell Reports 43, 113676, January 23, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER PMA Sigma-Aldrich Cat# P1585 Ionomycin Sigma-Aldrich Cat# I9657 Monensin Invitrogen Cat# 00-4505-51 Brefeldin A Invitrogen Cat# 00-4506-51 Collagenase IV Sigma Cat# C5138 DNAse I Roche Cat# 11284932001 Percoll Cytiva Cat# 17089101 LIVE DEAD Fixable Blue stain Invitrogen Cat# L34962 Insulin Novo Nordisk Cat# GR7B338 D-(+)-Glucose Sigma Cat# G8270 TRITC Dextran Sigma-Aldrich Cat# T1287 FCS Eurobio scientific Cat# CVFSVF00-01 Opti-MEM + GlutaMAX Gibco Cat# 51985-026 TaqMan Universal PCR Master Mix Applied Biosystems Cat# 4304437 P/S Gibco Cat# 15070-063 2-mercaptoethanol Gibco Cat# 31350-010 Critical commercial assays FITC BrdU Flow Kit BD Biosciences Cat# 51-2354AK; RRID:AB_2617060 CellTrace Violet Cell Proliferation Kit Invitrogen Cat# C34557 Luminex Mouse Premixed Multi-Analyte Kit R&D Systems Cat#LXSAMSM-16 Mouse ProcartaPlex Mix&Match 13-plex Invitrogen Cat# PPX-13-MXZTE2Y FITC Annexin V Apoptosis Detection Kit I BD Biosciences Cat# 556547; RRID:AB_2869082 PrimeScript RT Reagent Kit Takara Cat# RR037B Nuclear Transcription Factor Buffer Set BioLegend Cat# 424401 RNEasy Micro kit QIAGEN Cat# 74004 Experimental models: Organisms/strains Mouse C57BL/6j Jackson Laboratory Strain #:000664; RRID:IMSR_JAX:000664 Mouse B6.129S6(Cg)-Spp1tm1Blh/J Jackson Laboratory Strain #:004936; RRID:IMSR_JAX:004936 Mouse C57BL/6J-Myd88tm1Aki Dr Laurent Debarbieux, Institut Pasteur, Paris, France N/A Mouse B6(Cg)-Il15tm1.2Nsl/J Jackson Laboratory Strain #:034239; RRID:IMSR_JAX:034239 Mouse Ifng / Dr Philippe Bousso, Institut Pasteur, Paris, France N/A Oligonucleotides Acta2 Applied Biosystems Mm00725412_s1 Arg1 Applied Biosystems Mm00475988_m1 Bcl2 Applied Biosystems Mm00477631_m1 Ccl2 Applied Biosystems Mm00441242_m1 Col1a1 Applied Biosystems Mm00801666_g1 Cxcl9 Applied Biosystems Mm00434946_m1 Cxcl11 Applied Biosystems Mm00444662_m1 Cxcl10 Applied Biosystems Mm00445235_m1 Cxcr3 Applied Biosystems Mm99999054_s1 Eomes Applied Biosystems Mm01351984_m1 Il1b Applied Biosystems Mm00434228_m1 Il10 Applied Biosystems Mm01288386_m1 Il15 Applied Biosystems Mm00434210_m1 Il15Ra Applied Biosystems Mm04336046_m1 (Continued on next page) Cell Reports 43, 113676, January 23, 2024 19



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