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negative selection macs separation kit  (Miltenyi Biotec)


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    Miltenyi Biotec negative selection macs separation kit
    Negative Selection Macs Separation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+separation+kit/B+Cell+Isolation+Kit+II%2C+human/pmc13130636-396-6-11
    Average 96 stars, based on 710 article reviews
    negative selection macs separation kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Diabetes mellitus exacerbates myasthenia gravis by upregulating CD40 expression on B cells through the RAGE–HIF-1α–HK2 pathway
    Article Snippet: Background: Diabetes mellitus (DM) is a risk factor for myasthenia gravis (MG) and is known to enhance humoral immune response and antibody production in experimental autoimmune myasthenia gravis (EAMG) rats, possibly due to the interaction between B-cell CD40 and T follicular helper cells (Tfh).. This study was aimed to investigate the mechanism by which DM modulates B-cell CD40 regulation in individuals with MG. Methods: Blood samples from MG patients were collected to study changes in B cell subtypes, CD40 expression, and serum cytokine levels.. Rat models were used to examine B cell CD40 expression and identify gene differences between groups.

    Immune Cell:

    Article Title: Chemerin - CMKLR1 signaling contributes to immune metabolic dysfunction in salt-sensitive hypertension.
    Article Snippet: .. Building upon methodologies described in previous studies investigating immune cell behavior under varying sodium conditions, heparinized blood samples (40 mL) were collected from study participants, and peripheral blood mononuclear cells (PBMCs) were separated using a density-gradient centrifugation method with Ficollbased reagents.38,39 Demographic information of the participants has been previously reported.40 To further isolate monocytes, PBMCs underwent magnetic labeling followed by negative selection, utilizing the Miltenyi Biotec monocyte isolation kit (Miltenyi Biotec, Cat# 130-091-151). ..

    Blood/Plasma/Serum Samples:

    Article Title: Chemerin - CMKLR1 signaling contributes to immune metabolic dysfunction in salt-sensitive hypertension.
    Article Snippet: .. Building upon methodologies described in previous studies investigating immune cell behavior under varying sodium conditions, heparinized blood samples (40 mL) were collected from study participants, and peripheral blood mononuclear cells (PBMCs) were separated using a density-gradient centrifugation method with Ficollbased reagents.38,39 Demographic information of the participants has been previously reported.40 To further isolate monocytes, PBMCs underwent magnetic labeling followed by negative selection, utilizing the Miltenyi Biotec monocyte isolation kit (Miltenyi Biotec, Cat# 130-091-151). ..

    Centrifugation:

    Article Title: Chemerin - CMKLR1 signaling contributes to immune metabolic dysfunction in salt-sensitive hypertension.
    Article Snippet: .. Building upon methodologies described in previous studies investigating immune cell behavior under varying sodium conditions, heparinized blood samples (40 mL) were collected from study participants, and peripheral blood mononuclear cells (PBMCs) were separated using a density-gradient centrifugation method with Ficollbased reagents.38,39 Demographic information of the participants has been previously reported.40 To further isolate monocytes, PBMCs underwent magnetic labeling followed by negative selection, utilizing the Miltenyi Biotec monocyte isolation kit (Miltenyi Biotec, Cat# 130-091-151). ..

    Labeling:

    Article Title: Chemerin - CMKLR1 signaling contributes to immune metabolic dysfunction in salt-sensitive hypertension.
    Article Snippet: .. Building upon methodologies described in previous studies investigating immune cell behavior under varying sodium conditions, heparinized blood samples (40 mL) were collected from study participants, and peripheral blood mononuclear cells (PBMCs) were separated using a density-gradient centrifugation method with Ficollbased reagents.38,39 Demographic information of the participants has been previously reported.40 To further isolate monocytes, PBMCs underwent magnetic labeling followed by negative selection, utilizing the Miltenyi Biotec monocyte isolation kit (Miltenyi Biotec, Cat# 130-091-151). ..

    Selection:

    Article Title: Chemerin - CMKLR1 signaling contributes to immune metabolic dysfunction in salt-sensitive hypertension.
    Article Snippet: .. Building upon methodologies described in previous studies investigating immune cell behavior under varying sodium conditions, heparinized blood samples (40 mL) were collected from study participants, and peripheral blood mononuclear cells (PBMCs) were separated using a density-gradient centrifugation method with Ficollbased reagents.38,39 Demographic information of the participants has been previously reported.40 To further isolate monocytes, PBMCs underwent magnetic labeling followed by negative selection, utilizing the Miltenyi Biotec monocyte isolation kit (Miltenyi Biotec, Cat# 130-091-151). ..

    Article Title: In primary human lymphocytes, the triazole fungicide cyproconazole impairs regulatory B cell interleukin-10 expression and induces T cell senescence.
    Article Snippet: Triazole fungicides are applied worldwide, yet their effects on human lymphocytes remain poorly characterised.. We examined whether cyproconazole impairs regulatory B cell function and induces T cell senescence.. Purified B and T lymphocytes were exposed to cyproconazole (30–200 μM, 48–72 h).

    Isolation:

    Article Title: Chemerin - CMKLR1 signaling contributes to immune metabolic dysfunction in salt-sensitive hypertension.
    Article Snippet: .. Building upon methodologies described in previous studies investigating immune cell behavior under varying sodium conditions, heparinized blood samples (40 mL) were collected from study participants, and peripheral blood mononuclear cells (PBMCs) were separated using a density-gradient centrifugation method with Ficollbased reagents.38,39 Demographic information of the participants has been previously reported.40 To further isolate monocytes, PBMCs underwent magnetic labeling followed by negative selection, utilizing the Miltenyi Biotec monocyte isolation kit (Miltenyi Biotec, Cat# 130-091-151). ..

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: .. PBMCs were thawed and naïve‐enriched B cells were isolated using the Naïve B Cell Isolation Kit II human (Miltenyi Biotec, #130‐091‐150) with LS columns (Miltenyi Biotec, #130‐042‐401), according to manufacturer's instructions. .. Purified naïve‐enriched B cells were adjusted to 10 6 cells/mL and labelled with 0.25 μM CTV (Invitrogen, # C34557 ) at 37°C for 20 min, according to manufacturer's instructions and cultured in complete (c)RMPI media containing 10% FBS, 2 mM L‐glutamine (Sigma‐Aldrich, #G7513), 1× penicillin–streptomycin (Sigma‐Aldrich, #P0781), and 50 μM 2‐Mercaptoethanol (Thermofisher, #31350‐010).

    Article Title: B cell CD19 is transferred between immune cells in mice and humans
    Article Snippet: .. For the generation of human apoptotic B cells, PBMCs were generated from healthy donors, and B cells were isolated using the B Cell Isolation Kit II (Miltenyi; #130-091-151), according to manufacturer ́s instructions. ..

    Cell Isolation:

    Article Title: Senescent Fibroblasts Impair B Cell Proliferation via Cell–Cell Contact
    Article Snippet: .. PBMCs were thawed and naïve‐enriched B cells were isolated using the Naïve B Cell Isolation Kit II human (Miltenyi Biotec, #130‐091‐150) with LS columns (Miltenyi Biotec, #130‐042‐401), according to manufacturer's instructions. .. Purified naïve‐enriched B cells were adjusted to 10 6 cells/mL and labelled with 0.25 μM CTV (Invitrogen, # C34557 ) at 37°C for 20 min, according to manufacturer's instructions and cultured in complete (c)RMPI media containing 10% FBS, 2 mM L‐glutamine (Sigma‐Aldrich, #G7513), 1× penicillin–streptomycin (Sigma‐Aldrich, #P0781), and 50 μM 2‐Mercaptoethanol (Thermofisher, #31350‐010).

    Article Title: Functional exhaustion of human follicular cytotoxic T cells is associated with CEACAM1 expression and dysregulation of the SHP1–LCK–ZAP70 signaling axis
    Article Snippet: .. The human B cell isolation kit II (Miltenyi Biotec, 130-091-151) was used. .. For every 107 PBMCs, 40 μL of separation buffer was added to resuspend the cells, followed by the addition of 10 μL of REAlease CD19-Biotin, incubated at room temperature for 5 min. Then, 20 μL of CD19 Anti-Biotin MicroBeads was added and incubated at room temperature for another 5 min.

    Article Title: B cell CD19 is transferred between immune cells in mice and humans
    Article Snippet: .. For the generation of human apoptotic B cells, PBMCs were generated from healthy donors, and B cells were isolated using the B Cell Isolation Kit II (Miltenyi; #130-091-151), according to manufacturer ́s instructions. ..

    Article Title: In primary human lymphocytes, the triazole fungicide cyproconazole impairs regulatory B cell interleukin-10 expression and induces T cell senescence.
    Article Snippet: Triazole fungicides are applied worldwide, yet their effects on human lymphocytes remain poorly characterised.. We examined whether cyproconazole impairs regulatory B cell function and induces T cell senescence.. Purified B and T lymphocytes were exposed to cyproconazole (30–200 μM, 48–72 h).

    Article Title: Somatic Evolution of a Germline Antibody Expands its Breadth to Neutralize Early SARS‐CoV‐2 Omicron Variants
    Article Snippet: .. B cells were enriched from the PBMC samples according to the manufacture's protocol (B Cell Isolation Kit II, human, Miltenyi Biotec). ..

    Generated:

    Article Title: B cell CD19 is transferred between immune cells in mice and humans
    Article Snippet: .. For the generation of human apoptotic B cells, PBMCs were generated from healthy donors, and B cells were isolated using the B Cell Isolation Kit II (Miltenyi; #130-091-151), according to manufacturer ́s instructions. ..

    Purification:

    Article Title: In primary human lymphocytes, the triazole fungicide cyproconazole impairs regulatory B cell interleukin-10 expression and induces T cell senescence.
    Article Snippet: Triazole fungicides are applied worldwide, yet their effects on human lymphocytes remain poorly characterised.. We examined whether cyproconazole impairs regulatory B cell function and induces T cell senescence.. Purified B and T lymphocytes were exposed to cyproconazole (30–200 μM, 48–72 h).



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