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magnetic cell separation kits  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec magnetic cell separation kits
    Magnetic Cell Separation Kits, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+separation+kit/Myeloid+Dendritic+Cell+Isolation+Kit%2C+human/pm42520804-271-13-17
    Average 94 stars, based on 37 article reviews
    magnetic cell separation kits - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Isolation:

    Article Title: A Potent Inhibitor of Human Cytomegalovirus Infection Works Post-Entry Specifically in Differentiating Myelo-Monocytic Cells
    Article Snippet: .. Isolation of primary dendritic cells followed the same initial procedure for isolation of PBMC and was performed on the CD14 neg fraction collected after CD14+ monocyte isolation using a specific myeloid dendritic cell isolation kit (Miltenyi Biotech, Bisley, GU24 9DR, UK). .. Briefly, cells are incubated with a non-DC depletion cocktail and then biotin microbeads and purified on an LD column (Miltenyi Biotech, Bisley, GU24 9DR, UK) with unbound cells representing a population enriched for CD141 and CD1c expressing DCs.

    Article Title: Tumor-induced dendritic cell deregulation perturbs T cell proliferation and predicts clinical outcome in acute lymphoblastic leukemia.
    Article Snippet: Cells were cultured in complete RPMI-1640 medium (10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin) and supplemented with Gibco non-essential amino acids (1X), L-glutamine (2 mM), sodium pyruvate (1 mM), and 100 IU/mL of recombinant human IL-2 (Peprotech). .. cDCs were isolated from PBMCs of healthy donors or patients with ALL using magnetic cell separation kits (Miltenyi Biotec# 130-094- 487), following the manufacturer’s protocols. .. The NK-92 cell line was purchased from ATCC, routinely tested, and maintained under mycoplasma-free conditions.

    Article Title: IgA autoantibodies promote inflammation, Th17 polarization and fibrotic responses in hidradenitis suppurativa.
    Article Snippet: Cellswere incubated for 24 or 72 h, after which supernatants were collected and analyzed for TNF-α, IL-6, and IL-1β using commercial ELISA kits (Invitrogen). .. RNA was isolated for transcriptomic profiling by RNA sequencing, and culture supernatants were collected and analyzed for collagen type I alpha 1 (COL1A1) levels using ELISA. mDC and CD4 T cell co-culture Myeloid dendritic cells (mDCs) and CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from healthy control donors using the Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, cat no. 130-094-487) and the CD4+ T Cell Isolation Kit (Miltenyi Biotec, cat no. 130-096-533), respectively, following the manufacturer’s instructions. ..

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 10 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 105 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: HSD11B1 overexpression in dendritic cells and stromal cells relates to endometriosis by inhibiting dendritic cell proliferation and maturation.
    Article Snippet: .. The myeloid DCs were isolated using Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) through negative selection. ..

    Cell Isolation:

    Article Title: A Potent Inhibitor of Human Cytomegalovirus Infection Works Post-Entry Specifically in Differentiating Myelo-Monocytic Cells
    Article Snippet: .. Isolation of primary dendritic cells followed the same initial procedure for isolation of PBMC and was performed on the CD14 neg fraction collected after CD14+ monocyte isolation using a specific myeloid dendritic cell isolation kit (Miltenyi Biotech, Bisley, GU24 9DR, UK). .. Briefly, cells are incubated with a non-DC depletion cocktail and then biotin microbeads and purified on an LD column (Miltenyi Biotech, Bisley, GU24 9DR, UK) with unbound cells representing a population enriched for CD141 and CD1c expressing DCs.

    Article Title: IgA autoantibodies promote inflammation, Th17 polarization and fibrotic responses in hidradenitis suppurativa.
    Article Snippet: Cellswere incubated for 24 or 72 h, after which supernatants were collected and analyzed for TNF-α, IL-6, and IL-1β using commercial ELISA kits (Invitrogen). .. RNA was isolated for transcriptomic profiling by RNA sequencing, and culture supernatants were collected and analyzed for collagen type I alpha 1 (COL1A1) levels using ELISA. mDC and CD4 T cell co-culture Myeloid dendritic cells (mDCs) and CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from healthy control donors using the Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, cat no. 130-094-487) and the CD4+ T Cell Isolation Kit (Miltenyi Biotec, cat no. 130-096-533), respectively, following the manufacturer’s instructions. ..

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 10 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 105 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: Autoinflammation in patients with leukocytic CBL loss of heterozygosity is caused by constitutive ERK-mediated monocyte activation
    Article Snippet: .. Monocytes, B cells and mDCs were obtained by the magnetic sorting of total fresh PBMCs with CD14 microbeads (Miltenyi Biotec, #130-050-201), CD19 microbeads (Miltenyi Biotec, #130-050-301) and the Human Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, # 130- 094-487) according to the manufacturer’s instruction. ..

    Article Title: Comparison of PU.1 genomic binding across immune cells reveals cell type-specific roles in autoimmune disease
    Article Snippet: A Ficoll-Paque density gradient was used to isolate mononuclear cells from this preparation. .. A Myeloid Dendritic Cell Isolation Kit (130-094-487, Miltenyi Biotec) was then used to isolate myeloid dendritic cells from this sample, following manufacturer instructions. .. Human primary eosinophils were isolated from peripheral blood using an Eosinophil Isolation Kit (130-092-010, Miltenyi Biotec) following Ficoll-based granulocyte separation.

    Article Title: HSD11B1 overexpression in dendritic cells and stromal cells relates to endometriosis by inhibiting dendritic cell proliferation and maturation.
    Article Snippet: .. The myeloid DCs were isolated using Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) through negative selection. ..

    Magnetic Cell Separation:

    Article Title: Tumor-induced dendritic cell deregulation perturbs T cell proliferation and predicts clinical outcome in acute lymphoblastic leukemia.
    Article Snippet: Cells were cultured in complete RPMI-1640 medium (10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin) and supplemented with Gibco non-essential amino acids (1X), L-glutamine (2 mM), sodium pyruvate (1 mM), and 100 IU/mL of recombinant human IL-2 (Peprotech). .. cDCs were isolated from PBMCs of healthy donors or patients with ALL using magnetic cell separation kits (Miltenyi Biotec# 130-094- 487), following the manufacturer’s protocols. .. The NK-92 cell line was purchased from ATCC, routinely tested, and maintained under mycoplasma-free conditions.

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 10 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 105 cells/ml in AIM-V medium + 5% HuAB serum.

    RNA Sequencing:

    Article Title: IgA autoantibodies promote inflammation, Th17 polarization and fibrotic responses in hidradenitis suppurativa.
    Article Snippet: Cellswere incubated for 24 or 72 h, after which supernatants were collected and analyzed for TNF-α, IL-6, and IL-1β using commercial ELISA kits (Invitrogen). .. RNA was isolated for transcriptomic profiling by RNA sequencing, and culture supernatants were collected and analyzed for collagen type I alpha 1 (COL1A1) levels using ELISA. mDC and CD4 T cell co-culture Myeloid dendritic cells (mDCs) and CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from healthy control donors using the Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, cat no. 130-094-487) and the CD4+ T Cell Isolation Kit (Miltenyi Biotec, cat no. 130-096-533), respectively, following the manufacturer’s instructions. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: IgA autoantibodies promote inflammation, Th17 polarization and fibrotic responses in hidradenitis suppurativa.
    Article Snippet: Cellswere incubated for 24 or 72 h, after which supernatants were collected and analyzed for TNF-α, IL-6, and IL-1β using commercial ELISA kits (Invitrogen). .. RNA was isolated for transcriptomic profiling by RNA sequencing, and culture supernatants were collected and analyzed for collagen type I alpha 1 (COL1A1) levels using ELISA. mDC and CD4 T cell co-culture Myeloid dendritic cells (mDCs) and CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from healthy control donors using the Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, cat no. 130-094-487) and the CD4+ T Cell Isolation Kit (Miltenyi Biotec, cat no. 130-096-533), respectively, following the manufacturer’s instructions. ..

    Control:

    Article Title: IgA autoantibodies promote inflammation, Th17 polarization and fibrotic responses in hidradenitis suppurativa.
    Article Snippet: Cellswere incubated for 24 or 72 h, after which supernatants were collected and analyzed for TNF-α, IL-6, and IL-1β using commercial ELISA kits (Invitrogen). .. RNA was isolated for transcriptomic profiling by RNA sequencing, and culture supernatants were collected and analyzed for collagen type I alpha 1 (COL1A1) levels using ELISA. mDC and CD4 T cell co-culture Myeloid dendritic cells (mDCs) and CD4+ T cells were isolated from peripheral blood mononuclear cells (PBMCs) obtained from healthy control donors using the Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, cat no. 130-094-487) and the CD4+ T Cell Isolation Kit (Miltenyi Biotec, cat no. 130-096-533), respectively, following the manufacturer’s instructions. ..

    Gradient Centrifugation:

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 10 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 105 cells/ml in AIM-V medium + 5% HuAB serum.

    Selection:

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 10 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: Serum Arginase−1 is a predictive biomarker for melanoma patients and a surrogate indicator of systemic myeloid cell fitness
    Article Snippet: Data acquisition was performed on the Beckman Colter CytoFlex 6 L and analyzed using FlowJo v10.10.0 (FlowJo LLC, Ashland, OR). .. HD PBMCs isolated from buffy coats (Vitalant; Pittsburgh, PA) using Ficoll-HyPaque gradient centrifugation were processed by negative MACS selection using Pan-Monocyte and Myeloid Dendritic Cell Isolation Kits (Miltenyi Biotec; Gaithersburg, MD). .. Sorted monocytes, myeloid DCs, or corresponding depleted PBMC fractions were seeded in 48-well plates (Corning) at 5 × 105 cells/ml in AIM-V medium + 5% HuAB serum.

    Article Title: HSD11B1 overexpression in dendritic cells and stromal cells relates to endometriosis by inhibiting dendritic cell proliferation and maturation.
    Article Snippet: .. The myeloid DCs were isolated using Myeloid Dendritic Cell Isolation Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) through negative selection. ..



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