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Lucigen Corp e cloni replicator cells
E Cloni Replicator Cells, supplied by Lucigen Corp, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+replication/0+bac+cells+coli+e+electrocompetent+optimized+replicator+v2/pmc12416499-614-0-4
Average 86 stars, based on 1 article reviews
e cloni replicator cells - by Bioz Stars, 2026-10
86/100 stars

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Related Articles

Transformation Assay:

Article Title: Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates crosstalk between distinct signaling systems
Article Snippet: Note that pSMART (Lucigen), pMR120, and the derivatives are single-copy plasmids when transformed into E. coli ( 75 ). .. E. cloni replicator cells (Lucigen) are transformed with these plasmids and cultured with arabinose induction to increase the copy number for plasmid isolation. ..

Article Title: De novo-designed transmembrane domains tune engineered receptor functions
Article Snippet: The monomeric C-terminal portion of human L-selectin (CLS) ( ; ) was chosen as a negative control. .. Resulting plasmids were transformed into E. coli cloni cells (Lucigen), plated on agar plates containing 50 μl/ml spectinomycin followed by overnight growth in a 37°C at 200 rpm. ..

Article Title: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene.
Article Snippet: Metagenomic DNA (25–50 ng) in no more than 12.5 μL of agarose was used in ligation reactions set up according to the manufacturer’s directions using 25 ng pSMART BAC vector (Lucigen Corporation, Middleton, WI, USA) in a final volume of 50 μL. .. Ligation reactions (1 μL) were transformed into 20 μL of BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen Corporation) in a 1 mm gap cuvette using a BTX ECM 600 (BTX Harvard Apparatus, Holliston MD, USA) set with a resistance of 129 ohms and charging voltage of 1.2 kV. ..

Cell Culture:

Article Title: Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates crosstalk between distinct signaling systems
Article Snippet: Note that pSMART (Lucigen), pMR120, and the derivatives are single-copy plasmids when transformed into E. coli ( 75 ). .. E. cloni replicator cells (Lucigen) are transformed with these plasmids and cultured with arabinose induction to increase the copy number for plasmid isolation. ..

Plasmid Preparation:

Article Title: Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates crosstalk between distinct signaling systems
Article Snippet: Note that pSMART (Lucigen), pMR120, and the derivatives are single-copy plasmids when transformed into E. coli ( 75 ). .. E. cloni replicator cells (Lucigen) are transformed with these plasmids and cultured with arabinose induction to increase the copy number for plasmid isolation. ..

Isolation:

Article Title: Analysis of stress-induced small proteins in Escherichia coli reveals that YoaI mediates crosstalk between distinct signaling systems
Article Snippet: Note that pSMART (Lucigen), pMR120, and the derivatives are single-copy plasmids when transformed into E. coli ( 75 ). .. E. cloni replicator cells (Lucigen) are transformed with these plasmids and cultured with arabinose induction to increase the copy number for plasmid isolation. ..

BAC Assay:

Article Title: Identification and heterologous expression of the globomycin biosynthetic gene cluster.
Article Snippet: .. BAC-optimized replicator v2.0 electrocompetent E. coli cells (Lucigen, Middleton, USA; 60,210–1) were used during the cloning of the globomycin BGC. ..

Article Title: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene.
Article Snippet: Metagenomic DNA (25–50 ng) in no more than 12.5 μL of agarose was used in ligation reactions set up according to the manufacturer’s directions using 25 ng pSMART BAC vector (Lucigen Corporation, Middleton, WI, USA) in a final volume of 50 μL. .. Ligation reactions (1 μL) were transformed into 20 μL of BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen Corporation) in a 1 mm gap cuvette using a BTX ECM 600 (BTX Harvard Apparatus, Holliston MD, USA) set with a resistance of 129 ohms and charging voltage of 1.2 kV. ..

Cloning:

Article Title: Identification and heterologous expression of the globomycin biosynthetic gene cluster.
Article Snippet: .. BAC-optimized replicator v2.0 electrocompetent E. coli cells (Lucigen, Middleton, USA; 60,210–1) were used during the cloning of the globomycin BGC. ..

Ligation:

Article Title: Cloning and identification of novel hydrolase genes from a dairy cow rumen metagenomic library and characterization of a cellulase gene.
Article Snippet: Metagenomic DNA (25–50 ng) in no more than 12.5 μL of agarose was used in ligation reactions set up according to the manufacturer’s directions using 25 ng pSMART BAC vector (Lucigen Corporation, Middleton, WI, USA) in a final volume of 50 μL. .. Ligation reactions (1 μL) were transformed into 20 μL of BAC-Optimized Replicator v2.0 Electrocompetent Cells (Lucigen Corporation) in a 1 mm gap cuvette using a BTX ECM 600 (BTX Harvard Apparatus, Holliston MD, USA) set with a resistance of 129 ohms and charging voltage of 1.2 kV. ..



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