nanomolar synthesis in droplet microarrays with uv-triggered on-chip cell screening  (Verlag GmbH)

Journal: Stem Cell Research & Therapy

Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

doi: 10.1186/s13287-015-0056-7

Figure Lengend Snippet: RT q-PCR of a subset of genes from the microarray. Results from RT q-PCR analysis of a subset of up- or down-regulated genes in permanent pulp cells versus deciduous pulp cells. The data agree with the results obtained in the microarray experiments, and were all significantly different, P ≤0.05, Student’s t-test.

Article Snippet: Samples were analyzed using a PIQOR Stem Cell Microarray chip (Miltenyi Biotec, Auburn, CA, USA).

Techniques: Microarray

Journal: Stem Cell Research & Therapy

Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

doi: 10.1186/s13287-015-0056-7

Figure Lengend Snippet: Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec : Pulp cells from deciduous teeth, n = 3 Perm : Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.

Article Snippet: Samples were analyzed using a PIQOR Stem Cell Microarray chip (Miltenyi Biotec, Auburn, CA, USA).

Techniques: Western Blot, Expressing, Microarray

Journal: Stem Cell Research & Therapy

Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

doi: 10.1186/s13287-015-0056-7

Figure Lengend Snippet: RT q-PCR of a subset of genes from the microarray. Results from RT q-PCR analysis of a subset of up- or down-regulated genes in permanent pulp cells versus deciduous pulp cells. The data agree with the results obtained in the microarray experiments, and were all significantly different, P ≤0.05, Student’s t-test.

Article Snippet: A total of 1 μg RNA for each sample was used for amplification and further analysis with the PIQOR stem cell microarray chip, followed by detection with a laser scanner (Agilent Technologies, Santa Clara, CA, USA).

Techniques: Microarray

Journal: Stem Cell Research & Therapy

Article Title: Molecular differences between stromal cell populations from deciduous and permanent human teeth

doi: 10.1186/s13287-015-0056-7

Figure Lengend Snippet: Western blot analysis of protein expression. Western blot analysis of proteins reflects the results observed in the microarray and RT q-PCR gene experiments. Note that HMGA2 protein is low or non-existent in permanent pulp cells. Quantification on the intensity levels of the Western blot bands was performed, B-E. A) Western blot bands, B) HMGA2, C) CDC2A, D) FABE, E) SERPINF1. Dec : Pulp cells from deciduous teeth, n = 3 Perm : Pulp cells from permanent teeth, n = 3. *indicates P ≤ 0.05, unpaired Student’s t-test.

Article Snippet: A total of 1 μg RNA for each sample was used for amplification and further analysis with the PIQOR stem cell microarray chip, followed by detection with a laser scanner (Agilent Technologies, Santa Clara, CA, USA).

Techniques: Western Blot, Expressing, Microarray