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cell fixation solution  (Elabscience Biotechnology)


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    Elabscience Biotechnology cell fixation solution
    Cell Fixation Solution, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+fixation+solution/Tissue+Fixation+Solution+-+4%25+Paraformaldehyde/pmc11683131-86-5-9
    Average 96 stars, based on 14 article reviews
    cell fixation solution - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Staining:

    Article Title: A transcriptomic analysis of dental pulp stem cell senescence in vitro
    Article Snippet: .. The cells were immobilized using a 4% paraformaldehyde solution (Elabscience, Wuhan, China), washed with PBS, and then stained with Oil Red O. .. Following observation and capture of the images under a light microscope, the stained lipid droplets were dissolved in isopropanol (Sigma-Aldrich) at room temperature, the decolorized solution collected, and the absorbance at 510 nm measured using an enzyme marker.

    Article Title: Annao Pingchong decoction attenuates oxidative stress and neuronal apoptosis following intracerebral hemorrhage via RAGE-NOX2/4 axis
    Article Snippet: The tissues were then embedded in paraffin, and 5 μm thick coronal sections were obtained using a microtome (HM 325, Thermo Fisher, China). .. Cell samples were fixed with cell fixation solution (E-IR-R114, Elabscience) for 30 min. After dewaxing and rehydration, the sections were stained with a Nissl (G1434, Solarbio) and TUNEL staining kit (A112, Vazyme) following the manufacturer’s instructions. ..

    Article Title: A transcriptomic analysis of dental pulp stem cell senescence in vitro
    Article Snippet: After the cells reached 100% confluence, the original medium was removed and lipid induction was carried out for 14 d using a lipid-forming differentiation-inducing medium (Pricella) following the manufacturer’s instructions. .. The cells were fixed using 4% paraformaldehyde solution (Elabscience), washed with PBS, and stained with ORO. .. Following observation and capture of images under a light microscope, the stained lipid droplets were dissolved in isopropanol (Sigma-Aldrich) at room temperature, the decolourised solution was collected, and the absorbance at 520 nm was measured using an enzyme label reader.

    Incubation:

    Article Title: An integrated “Engage & Evasion” approach for mononuclear phagocyte system escape and efficient extracellular vesicle therapy
    Article Snippet: To assess cellular uptake, EVs were labeled with DiD (1 μM, Solarbio) and reisolated. .. Recipient cells were incubated with these DiD-labeled EVs (200 μg/mL) for 4 h, followed by fixation with 4% paraformaldehyde (Elabscience). .. Cellular internalization was visualized with confocal microscope (LSM880, Zeiss) and quantified via flow cytometry (Millipore Guava easyCyte) [ , ].

    other:

    Article Title: Chenodeoxycholic Acid-Mediated neuroprotection via α-synuclein and BDNF Modulation in MPTP-Induced mouse model of Parkinson's disease.
    Article Snippet: Parkinson’s disease (PD) remains a major challenge in the field of neurodegenerative diseases and requires innovative therapeutic approaches.. In this study, we investigated the therapeutic potential of chenodeoxycholic acid (CDCA) in PD using a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced mouse model. CDCA, a naturally occurring bile acid, has previously shown promise in various neurological disorders by reducing neuronal degeneration and promoting neuronal health, however its utility in PD has not been studied.. We divided mice into a control group, an MPTP-induced PD model and a treatment group injected with CDCA.

    TUNEL Assay:

    Article Title: Annao Pingchong decoction attenuates oxidative stress and neuronal apoptosis following intracerebral hemorrhage via RAGE-NOX2/4 axis
    Article Snippet: The tissues were then embedded in paraffin, and 5 μm thick coronal sections were obtained using a microtome (HM 325, Thermo Fisher, China). .. Cell samples were fixed with cell fixation solution (E-IR-R114, Elabscience) for 30 min. After dewaxing and rehydration, the sections were stained with a Nissl (G1434, Solarbio) and TUNEL staining kit (A112, Vazyme) following the manufacturer’s instructions. ..



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