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Becton Dickinson cellfix™ cell fixation solution

Cellfix™ Cell Fixation Solution, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+fixation+solution/cell+fixation+solution/pmc09832874-43-0-5
Average 90 stars, based on 1 article reviews
cellfix™ cell fixation solution - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "A novel phosphocholine‐mimetic inhibits a pro‐inflammatory conformational change in C‐reactive protein"

Article Title: A novel phosphocholine‐mimetic inhibits a pro‐inflammatory conformational change in C‐reactive protein

Journal: EMBO Molecular Medicine

doi: 10.15252/emmm.202216236


Figure Legend Snippet:

Techniques Used: Recombinant, Crystallization Assay, Staining, Software, Immunofluorescence, Western Blot

Related Articles

Incubation:

Article Title: An Unbiased Flow Cytometry-Based Approach to Assess Subset-Specific Circulating Monocyte Activation and Cytokine Profile in Whole Blood
Article Snippet: .. After stimulation, we immediately added 50 μL of cell fixation solution (BD CellFIXTM) to all samples and incubated them for 5 min at room temperature (21°C) to stop further cell activation. ..

Article Title: An Unbiased Flow Cytometry-Based Approach to Assess Subset-Specific Circulating Monocyte Activation and Cytokine Profile in Whole Blood
Article Snippet: .. After stimulation, we immediately added 50 μL of cell fixation solution (BD CellFIXTM) at room temperature to all samples and incubated them for 5 min at room temperature (21°C) to stop further cell activation. ..

Article Title: Peripheral T cell populations are differentially affected in monogenic autoinflammatory syndromes and gout
Article Snippet: Cells were washed with the washing solution (BD Bioscience) and antibody mix in the washing solution was added. .. After 30 minutes incubation at 4 °C in the dark, the cells were washed and stored at 4 °C in cell fixation solution (BD Bioscience) until analysis. .. Data Analysis 6 Flow cytometry data was analyzed using FlowJo (version 10.0) software.

Activation Assay:

Article Title: An Unbiased Flow Cytometry-Based Approach to Assess Subset-Specific Circulating Monocyte Activation and Cytokine Profile in Whole Blood
Article Snippet: .. After stimulation, we immediately added 50 μL of cell fixation solution (BD CellFIXTM) to all samples and incubated them for 5 min at room temperature (21°C) to stop further cell activation. ..

Article Title: An Unbiased Flow Cytometry-Based Approach to Assess Subset-Specific Circulating Monocyte Activation and Cytokine Profile in Whole Blood
Article Snippet: .. After stimulation, we immediately added 50 μL of cell fixation solution (BD CellFIXTM) at room temperature to all samples and incubated them for 5 min at room temperature (21°C) to stop further cell activation. ..

Lysis:

Article Title: An impaired inflammatory cytokine response to gram-negative LPS in human neonates is associated with the defective TLR-mediated signaling pathway.
Article Snippet: Purpose Human neonates are highly susceptible to a wide range of infections, which has been attributed to deficiencies in their innate and adaptive immunity.. In contrast to the welldocumented immaturity in neonatal adaptive immunity, deficiencies in their innate immunity are less defined.. This study examined the inflammatory response of neonatal monocytes to bacterial lipopolysaccharide (LPS) and peptidoglycan (PGN) stimulation and discriminated the underlying Tolllike receptor (TLR)-mediated signal transduction pathways.

Flow Cytometry:

Article Title: Improvement of monoclonal antibody-immobilized granulocyte antigen assay for the detection of anti-HNA-1 alloantibodies.
Article Snippet: BACKGROUND: Currently, the gold standard for the identification of antibodies against human neutrophil antigens (HNAs) is the monoclonal antibody–immobilized granulocyte antigen (MAIGA) assay.. However, the handling of this assay is laborious and therefore cumbersome for the rapid screening of neutrophil antibodies.. STUDY DESIGN AND METHODS: In this study, we simplified the performance of the conventional MAIGA procedure and approved it for the identification of antiHNA-1 with HNA-1–typed neutrophils and stable transfected (HEK293) cell line expressing HNA-1a, -1b, and -1c.

Modification:

Article Title: Improvement of monoclonal antibody-immobilized granulocyte antigen assay for the detection of anti-HNA-1 alloantibodies.
Article Snippet: BACKGROUND: Currently, the gold standard for the identification of antibodies against human neutrophil antigens (HNAs) is the monoclonal antibody–immobilized granulocyte antigen (MAIGA) assay.. However, the handling of this assay is laborious and therefore cumbersome for the rapid screening of neutrophil antibodies.. STUDY DESIGN AND METHODS: In this study, we simplified the performance of the conventional MAIGA procedure and approved it for the identification of antiHNA-1 with HNA-1–typed neutrophils and stable transfected (HEK293) cell line expressing HNA-1a, -1b, and -1c.

other:

Article Title: A novel phosphocholine‐mimetic inhibits a pro‐inflammatory conformational change in C‐reactive protein
Article Snippet: CellFIXTM cell fixation solution , BD Biosciences , https://www.bdbiosciences.com/en‐de/products/reagents/flow‐cytometry‐reagents/clinical‐diagnostics/buffers‐and‐supporting‐reagents‐ivd‐ce‐ivd/bd‐cellfix‐10x‐concentrate.340181.



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