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MultiSciences Biotech Co Ltd cell cycle staining kit ccs012
Cell Cycle Staining Kit Ccs012, supplied by MultiSciences Biotech Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+cycle+staining+kit/cell+cycle+staining+kit+ccs012/pm38467320-87-3-9
Average 90 stars, based on 1 article reviews
cell cycle staining kit ccs012 - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Flow Cytometry:

Article Title: Targeted Degradation of the Estrogen Receptor α through Unconventional Coactivator Binding Site (CBS).
Article Snippet: Inhibiting the estrogen receptor α (ERα) signaling axis constitutes a fundamental approach in therapy for ERαpositive breast cancer (ERα+ BC).. However, the emergence of intrinsic and acquired resistance to endocrine therapies poses a significant challenge.. Alternatively, targeting the coactivator binding site (CBS) of ERα instead of the conventional ligand binding domain (LBD) may directly disrupt the ERα transcription process, thereby circumventing resistance associated with LBD mutations.

Staining:

Article Title: Targeted Degradation of the Estrogen Receptor α through Unconventional Coactivator Binding Site (CBS).
Article Snippet: Inhibiting the estrogen receptor α (ERα) signaling axis constitutes a fundamental approach in therapy for ERαpositive breast cancer (ERα+ BC).. However, the emergence of intrinsic and acquired resistance to endocrine therapies poses a significant challenge.. Alternatively, targeting the coactivator binding site (CBS) of ERα instead of the conventional ligand binding domain (LBD) may directly disrupt the ERα transcription process, thereby circumventing resistance associated with LBD mutations.

Article Title: Targeting the splicing factor SNRPB inhibits endometrial cancer progression by retaining the POLD1 intron
Article Snippet: .. For the cell cycle assay, the cells were stained with propidium iodide following the protocol (CCS012, MultiSciences Biotech). .. The distribution of cell cycle phases across different groups was subsequently assessed via the Beckman CytoFLEX FCM (Beckman Coulter), with data analysis performed via ModFit LT software.

Article Title: KCNE3 Facilitates M1 Macrophage Polarization by Suppressing the Wnt/β-Catenin Pathway, Inhibiting Glioma Proliferation, Migration, and Invasion.
Article Snippet: The glioma microenvironment is critical for tumor growth, where reprogramming M2‐polarized tumor‐associated macrophages/microglia (TAMs) to an antitumor M1 phenotype represents a promising therapeutic strategy.. While the potassium channel regulatory subunit KCNE3 has been implicated in tumorigenesis across malignancies, its functional role in shaping the glioma microenvironment remains undefined.. Here, we leveraged transcriptome data from the Gene Expression Omnibus (GEO) to identify KCNE3 as a TAM‐enriched gene in gliomas.

other:

Article Title: Mechanism of berbamine-mediated DNA damage in synovial fibroblasts to alleviate rheumatoid arthritis.
Article Snippet: Rheumatoid arthritis (RA) is a common chronic autoimmune disease characterised by the proliferation and infiltration of fibroblast-like synoviocytes (FLS).. However, pharmaceutical approaches to inhibit FLS in the treatment of RA are limited.. Berbamine (BBM), a natural compound extracted from Phellodendron chinense Schneider, has demonstrated anti-tumour and anti-inflammatory effects.

Incubation:

Article Title: The role of TRAF2 in pan-cancer revealed by integrating informatics and experimental validation
Article Snippet: .. We added propidium iodide (PI, CCS012, MultiSciences Biotech Co., China) and permeabilization solution, and incubated the mixture for 30 min at 25°C in the dark. .. The cells were collected, and the cell cycle was analyzed using a flow cytometer (Attune NxT, Thermo Fisher Scientific Inc., United States).

Article Title: Overexpression of MAFG-AS1 in ovarian cancer promotes glucose metabolism reprogramming and malignant biological behavior of ovarian cancer cells by regulating HIF-1α.
Article Snippet: .. Subsequently, after a 24-h incubation period, the cells were treated with a cell cycle assay kit (CCS012, Multisciences, Hangzhou, China) following the manufacturer’s instructions. ..

Article Title: Overexpression of MAFG-AS1 in ovarian cancer promotes glucose metabolism reprogramming and malignant biological behavior of ovarian cancer cells by regulating HIF-1α
Article Snippet: .. Subsequently, after a 24-h incubation period, the cells were treated with a cell cycle assay kit (CCS012, Multisciences, Hangzhou, China) following the manufacturer's instructions. ..

Cell Cycle Assay:

Article Title: Overexpression of MAFG-AS1 in ovarian cancer promotes glucose metabolism reprogramming and malignant biological behavior of ovarian cancer cells by regulating HIF-1α.
Article Snippet: .. Subsequently, after a 24-h incubation period, the cells were treated with a cell cycle assay kit (CCS012, Multisciences, Hangzhou, China) following the manufacturer’s instructions. ..

Article Title: Overexpression of MAFG-AS1 in ovarian cancer promotes glucose metabolism reprogramming and malignant biological behavior of ovarian cancer cells by regulating HIF-1α
Article Snippet: .. Subsequently, after a 24-h incubation period, the cells were treated with a cell cycle assay kit (CCS012, Multisciences, Hangzhou, China) following the manufacturer's instructions. ..

Article Title: Targeting the splicing factor SNRPB inhibits endometrial cancer progression by retaining the POLD1 intron
Article Snippet: .. For the cell cycle assay, the cells were stained with propidium iodide following the protocol (CCS012, MultiSciences Biotech). .. The distribution of cell cycle phases across different groups was subsequently assessed via the Beckman CytoFLEX FCM (Beckman Coulter), with data analysis performed via ModFit LT software.



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In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).
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In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

Journal: Bioactive Materials

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

doi: 10.1016/j.bioactmat.2026.02.051

Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

Techniques: In Vitro, Fluorescence, Staining