apoptosis analysis kit  (Beyotime)

Journal: Bioactive Materials

Article Title: A dual-functional hydrogel integrating adhesive and lubricating interfaces for mitochondrial protection–Driven cartilage regeneration

doi: 10.1016/j.bioactmat.2026.02.051

Figure Lengend Snippet: In vitro antioxidant and mitochondrial homeostasis regulatory effects of AdHy@Pae. (A) Flow cytometric analysis of the cell cycle. (B–D) Quantitative statistics of the percentage of cells in G0/G1, S, and G2/M phases. (E) JC-1 fluorescence staining. (F) Quantitative analysis of JC-1 red/green fluorescence ratio (ΔΨm). (G) Flow cytometric detection of intracellular ROS using DCFH-DA probe. Data are shown as mean ± SD (n = 3, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001).

Article Snippet: After LPS stimulation (10 μg mL −1 , 12 h, ServiceBio, GC205009 ) to induce an inflammatory phenotype, cells were treated with hydrogel extracts (Hy, AdHy, AdHy@Pae) for 24 h. Cells were then washed twice with PBS, harvested, and fixed overnight at 4 °C in 70% cold ethanol; after washing, they were stained with 500 μL cell-cycle staining solution (MULTI SCIENCES, CCS012) for 30 min in the dark.

Techniques: In Vitro, Fluorescence, Staining