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Abbkine Inc cell cycle analysis kit
Proteomic profiling of EEDJF-treated colorectal cancer (CRC) cells. (A–C) Effects of EEDJF on the viability of HCT116, SW480, and HaCaT cells. (D) Volcano plot showing differentially expressed proteins (DEPs) between control and EEDJF-treated groups. (E) Hierarchical clustering <t>analysis</t> of DEPs. (F) Gene Ontology (GO) enrichment analysis of DEPs. (G) Top 20 enriched KEGG pathways associated with DEPs. (H) Quantitative analysis of <t>cell</t> <t>cycle-related</t> proteins. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Cell Cycle Analysis Kit, supplied by Abbkine Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+cycle+analysis+kit/cell+dead+double+kit+live+staining/pmc13265501-96-1-7
Average 86 stars, based on 1 article reviews
cell cycle analysis kit - by Bioz Stars, 2026-11
86/100 stars

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1) Product Images from "Integrated multi-omics analysis suggests the involvement of PI3K-Akt/p21 signaling in the anti-colorectal cancer effects of Diaphragma Juglandis Fructus extract"

Article Title: Integrated multi-omics analysis suggests the involvement of PI3K-Akt/p21 signaling in the anti-colorectal cancer effects of Diaphragma Juglandis Fructus extract

Journal: Frontiers in Pharmacology

doi: 10.3389/fphar.2026.1833123

Proteomic profiling of EEDJF-treated colorectal cancer (CRC) cells. (A–C) Effects of EEDJF on the viability of HCT116, SW480, and HaCaT cells. (D) Volcano plot showing differentially expressed proteins (DEPs) between control and EEDJF-treated groups. (E) Hierarchical clustering analysis of DEPs. (F) Gene Ontology (GO) enrichment analysis of DEPs. (G) Top 20 enriched KEGG pathways associated with DEPs. (H) Quantitative analysis of cell cycle-related proteins. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Figure Legend Snippet: Proteomic profiling of EEDJF-treated colorectal cancer (CRC) cells. (A–C) Effects of EEDJF on the viability of HCT116, SW480, and HaCaT cells. (D) Volcano plot showing differentially expressed proteins (DEPs) between control and EEDJF-treated groups. (E) Hierarchical clustering analysis of DEPs. (F) Gene Ontology (GO) enrichment analysis of DEPs. (G) Top 20 enriched KEGG pathways associated with DEPs. (H) Quantitative analysis of cell cycle-related proteins. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Techniques Used: Control

EEDJF induces G1/S cell cycle arrest in HCT116 cells. (A) Western blot analysis of cell cycle–related proteins (p21, Cyclin D1, and CDK4) following EEDJF treatment. (B) Densitometric quantification of protein expression shown in (A) . Band intensities were quantified using ImageJ software and normalized to β-actin. (C) Flow cytometric analysis of cell cycle distribution after EEDJF treatment. (D,E) Quantitative distribution of cells in G1, S, and G2/M phases. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
Figure Legend Snippet: EEDJF induces G1/S cell cycle arrest in HCT116 cells. (A) Western blot analysis of cell cycle–related proteins (p21, Cyclin D1, and CDK4) following EEDJF treatment. (B) Densitometric quantification of protein expression shown in (A) . Band intensities were quantified using ImageJ software and normalized to β-actin. (C) Flow cytometric analysis of cell cycle distribution after EEDJF treatment. (D,E) Quantitative distribution of cells in G1, S, and G2/M phases. Data are presented as mean ± SD from three independent biological experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

Techniques Used: Western Blot, Expressing, Software

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Staining:

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Article Snippet: .. Senescence-associated β -galactosidase (SA- β -Gal) staining was performed using the β -Galactosidase Staining Kit (Abbkine, Wuhan, China) according to the manufacturer's protocol. ..

Article Title: KDM8/c-Myc axis-mediated glucose metabolism reprogramming promotes the progression of ovarian cancer
Article Snippet: .. After fixation, cells were stained using the Cell Cycle Staining Kit (Abbkine) and analyzed by flow cytometer (BD Biosciences). ..

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Article Title: KDM8/c-Myc axis-mediated glucose metabolism reprogramming promotes the progression of ovarian cancer.
Article Snippet: .. After fixation, cells were stained using the Cell Cycle Staining Kit (Abbkine) and analyzed by flow cytometer (BD Biosciences). ..

Article Title: Trabecular-Like Scaffold Dictates Osteogenesis via Fluid Shear Stress-Induced Metabolic Reprogramming through the CAV1–HIF-1α Axis
Article Snippet: .. For live and dead staining, BMSCs cultured on Voronoi-based scaffolds were incubated with a commercial live and dead viability kit (KTA1001, Abbkine) containing calcein acetoxymethyl ester and PI, according to the manufacturer’s instructions. ..

Article Title: Self-navigated NIR-II thermophoretic liposome nanomotors for directed tumor delivery and enhanced chemotherapy
Article Snippet: Dulbecco's modified Eagle's media (DMEM), phosphate-buffered saline (PBS), trypsin, paraformaldehyde and MTT cell proliferation and cytotoxicity assay kit were purchased from KeyGEN Biotech (Nanjing, China). .. Calcein-AM/PI Double Stain Kit was purchased from Abbkine Scientific Co.,Ltd (Wuhan, China). .. Aqueous solutions used in the experiments were prepared with deionized (DI) H 2 O (18.1 M Ω cm, Millipore).

Article Title: Mechanically sensitized hydrogel microspheres trigger membrane receptor switch for cartilage repair.
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Flow Cytometry:

Article Title: KDM8/c-Myc axis-mediated glucose metabolism reprogramming promotes the progression of ovarian cancer
Article Snippet: .. After fixation, cells were stained using the Cell Cycle Staining Kit (Abbkine) and analyzed by flow cytometer (BD Biosciences). ..

Article Title: KDM8/c-Myc axis-mediated glucose metabolism reprogramming promotes the progression of ovarian cancer.
Article Snippet: .. After fixation, cells were stained using the Cell Cycle Staining Kit (Abbkine) and analyzed by flow cytometer (BD Biosciences). ..

Cell Culture:

Article Title: Trabecular-Like Scaffold Dictates Osteogenesis via Fluid Shear Stress-Induced Metabolic Reprogramming through the CAV1–HIF-1α Axis
Article Snippet: .. For live and dead staining, BMSCs cultured on Voronoi-based scaffolds were incubated with a commercial live and dead viability kit (KTA1001, Abbkine) containing calcein acetoxymethyl ester and PI, according to the manufacturer’s instructions. ..

Incubation:

Article Title: Trabecular-Like Scaffold Dictates Osteogenesis via Fluid Shear Stress-Induced Metabolic Reprogramming through the CAV1–HIF-1α Axis
Article Snippet: .. For live and dead staining, BMSCs cultured on Voronoi-based scaffolds were incubated with a commercial live and dead viability kit (KTA1001, Abbkine) containing calcein acetoxymethyl ester and PI, according to the manufacturer’s instructions. ..

Cell Cycle Assay:

Article Title: Integrated multi-omics analysis suggests the involvement of PI3K-Akt/p21 signaling in the anti-colorectal cancer effects of Diaphragma Juglandis Fructus extract
Article Snippet: Protein quantification and lysis reagents were from Boser Biotechnology (Wuhan, China). .. The cell cycle analysis kit was from Abbkine (Wuhan, China). .. Antibodies were purchased as follows: p-PI3K from Bioss (Beijing, China); PI3K and p21 from Proteintech (Wuhan, China); p-Akt, Cyclin D1, and CDK4 from Wanleibio (Shenyang, China); and total AKT from Cell Signaling Technology (MA, USA).



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