anti human cd28 (Bio X Cell)

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Bio X Cell anti human cd28
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Anti Human Cd28, supplied by Bio X Cell, used in various techniques. Bioz Stars score: 96/100, based on 502 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+cloning-based+transcriptome+analysis/InVivoMAb+anti-human+monkey+CD28/pmc07050437-5-0-5
Average 96 stars, based on 502 article reviews

anti human cd28 - by Bioz Stars, 2026-09

96/100 stars

Images

1) Product Images from "TLR8-mediated metabolic control of human Treg function: a mechanistic target for cancer immunotherapy"

Article Title: TLR8-mediated metabolic control of human Treg function: a mechanistic target for cancer immunotherapy

Journal: Cell metabolism

doi: 10.1016/j.cmet.2018.09.020

Figure Legend Snippet: KEY RESOURCES TABLE

Techniques Used: Derivative Assay, Recombinant, Reverse Transcription, Selection, Cell Based Assay, Software

Cell Culture:

Article Title: Noninvasive in vivo deoxycytidine kinase (dCK)-PET identifies tumor-draining lymph nodes upon immune checkpoint inhibitor therapy
Article Snippet: CD4 + and CD8 + T cells were isolated using a MACS column system (Miltenyi; Bergisch Gladbach, Germany) and resuspended in DMEM containing 1% 1 M HEPES buffer (PAN-Biotech GmbH, Aidenbach, Germany), 1% 100 mM sodium pyruvate solution, 1% MEM amino acid solution, and 500 μM β-mercaptoethanol (all from Sigma Aldrich). .. The cells were cultured in 6-well plates prepared 24 h in advance with either phosphate-buffered saline (PBS) supplemented with 0.5 μg/mL anti-CD3 and 5 μg/mL anti-CD28 (both purchased from BioXcell, Lebanon, NH, USA) or exclusively with PBS (naïve condition). .. On the subsequent day, 50 U/mL IL-2 (Novartis, Basel, CH) and 0.5 ng/mL IL-7 (Thermo Scientific, Waltham, MA, USA) were added to the cells cultured with anti-CD3/anti-CD28 mAbs.

Saline:

Concentration Assay:

Article Title: Inhibition of the cancer stem cell immune checkpoint SOAT1 suppresses regulatory T cell functions through a trans-cellular 20(S)-Hydroxycholesterol-GPR132 pathway in mice.
Article Snippet: .. Naïve CD4+ T cells were resuspended in RPMI 1640 medium, 10% FBS, 100 units mL−1 penicillin/streptomycin, 4 mM Lglutamine (Gibco, Cat#25030081), 1 mM sodium pyruvate (Gibco, Cat# 11360070), 50 μM 2- mercaptoethanol (Sigma–Aldrich, Cat#M3180),25 mM HEPES (Gibco, Cat#15630130), and 1 × MEM nonessential amino acid solution (Gibco, Cat#11140050) and plated into a 6-well plate previously coated with anti-CD3 antibodies (BioXcell, Cat# BE0001-1) at a final concentration of 10 μg mL−1 for 48 h. The cells were then induced for approximately 6 days using a culture medium supplemented with TGF-β at 5 ng mL−1 (Yeasen, Cat#92347ES50), IL-10 at 10 ng mL−1 (Yeasen, Cat#90149ES60), and anti-CD28 at 2 ng mL−1 (BioXcell, Cat# BE0015-1) to drive their differentiation into Foxp3-positive regulatory T cells. ..

Purification:

Article Title: The LXRβ/NF-κB axis reprograms CAR-T cells to resist exhaustion in the tumor microenvironment
Article Snippet: CD8+ T cells were isolated by negative selection with the EasySepTM Mouse CD8+ T Cell Isolation Kit and EasyEightsTM Magnet (STEMCELL Technologies). .. Purified CD8+ T cells were activated for 24 h with 5 μg/mL plate-bound anti-CD3 and anti-CD28 antibodies (Bio X Cell) before CAR-T cell generation. .. The pMIG-LXRβ retroviral vector was generated by inserting a synthetic LXRβ coding sequence (IDT gBlocks) into the pMSCV-IRES-eGFP backbone (Addgene #27490) using the NEBuilder HiFi DNA Assembly Kit (NEB).

Isolation:

Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies
Article Snippet: .. A total of 0.5 million isolated CD4 + T cells or CD8 + T cells was stimulated with plate-coated anti-CD3 (10 μg/ml; BioXCell, catalog no. BE0001-2) and anti-CD28 (BioXCell, catalog no. BE0291), rhIL-6 (100 ng/ml), rhIFN-α 2 (100 ng/ml), rhIL-12 (100 ng/ml), or the combination of rhIL-6, rhIFN-α 2 , and rhIL-12 for 5 days. ..

Article Title: Inflammatory arthritis irAE may represent a unique autoimmune disease primarily driven by T cells but likely not autoantibodies.
Article Snippet: .. A total of 0.5 million isolated CD4+ T cells or CD8+ T cells was stimulated with plate- coated anti- CD3 (10 μg/ml; BioXCell, catalog no. BE0001- 2) and anti- CD28 (BioXCell, catalog no. BE0291), rhIL- 6 (100 ng/ml), rhIFN- α2 (100 ng/ ml), rhIL- 12 (100 ng/ml), or the combination of rhIL- 6, rhIFN- α2, and rhIL- 12 for 5 days. ..

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