trypsin edta (Thermo Fisher)

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Thermo Fisher trypsin edta
Trypsin Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+cloning-based+transcriptome+analysis/Trypsin/pm35977508-294-162-166
Average 99 stars, based on 1 article reviews

trypsin edta - by Bioz Stars, 2026-09

99/100 stars

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Isolation:

Article Title: E. coli -expressed chicken IL-17A enhances respiratory innate immunity by inducing proinflammatory cytokines and interferons
Article Snippet: AlphaFold v2.3 (DeepMind) was used to construct the structural model. .. Primary chicken embryo fibroblasts (CEFs) were isolated from 11-day-old specific-pathogen-free (SPF) chicken embryos (obtained from Xinxing Dahuanong Poultry Egg Co., Ltd., with a certificate of quality conformity provided by the supplier) through trypsin-DNase digestion and maintained in Dulbecco's modified Eagle's medium (DMEM, Gibco, 11995065) supplemented with 10% fetal bovine serum (FBS, Gibco, 16140071) at 37°C under 5% CO2. .. Peripheral blood mononuclear cells (PBMCs) were isolated from the heparinized blood of 4-week-old chickens via Ficoll-Paque plus (GE Healthcare, 17144003) density gradient centrifugation and cultured in RPMI 1640 medium (Gibco, 61870036) containing 10% FBS.

Modification:

Saline:

Article Title: From N-of-1 to versatility in propionic acidemia: Antisense oligonucleotide-mediated skipping of a constitutive PCCA pseudoexon
Article Snippet: .. Forty-eight hours post-transfection, the fibroblasts were detached using 0.25% trypsin/EDTA solution (Thermo Fisher Scientific), washed once with phosphate-buffered saline, centrifuged to obtain a cell pellet of 1 × 10 6 cells, and flash-frozen in liquid nitrogen. ..

Passaging:

Article Title: Sustained release PLGA microspheres loaded with a bone-affinity Bmp2 enhance fracture healing and mitigate heterotopic ossification
Article Snippet: .. Passaging was performed every 2–3 days using 0.25% trypsin-EDTA (Gibco, USA) when cell confluence reached approximately 80–90%. ..

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