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fcs based supplement mix c 39226  (PromoCell)


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    PromoCell fcs based supplement mix c 39226
    Fcs Based Supplement Mix C 39226, supplied by PromoCell, used in various techniques. Bioz Stars score: 97/100, based on 522 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+base+medium/Endothelial+Cell+Growth+Medium+MV+2/pmc12893108-252-21-18
    Average 97 stars, based on 522 article reviews
    fcs based supplement mix c 39226 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Transferrin receptor 1 shedding by the pro-inflammatory iRhom-ADAM17 complex and ADAM10 regulates cellular iron uptake and ferroptosis.
    Article Snippet: .. The cells were cultured in endothelial cell growth medium MV2 with supplement pack (PromoCell), 60mg/l penicillin (Sigma-Aldrich) and 100mg/l streptomycin (Sigma-Aldrich) in a humidified incubator at 37 °C with 5% CO2. ..

    Article Title: Endothelin-1 signaling regulates chamber-specific mouse atrial cardiomyocyte cytokinesis and polyploidy
    Article Snippet: .. Fibroblasts were collected from the remaining cells by pre-plating on 10 cm Petri dishes for 1 h. Purified endothelial cells were cultured in endothelial cell growth medium MV2 (Promocell, #C-22022) and maintained in 10 cm Petri dishes (Sarstedt, 83.3902) coated with 1% gelatin (Sigma, G1890). ..

    Article Title: Diabetes impacts endothelial Weibel-Palade body biogenesis and VWF secretion
    Article Snippet: .. HUVECs: Pooled Human Umbilical Vein Cells (HUVECs) (PromoCell) cultured in basal endothelial cell growth media with supplement pack (PromoCell) and antimycotic (Gibco) added forming endothelial growth medium (EGM-2). hAECS: Primary human aortic endothelial cells (hAECs: lot.483z027.6) or dhAECs (lot.4727002.1) from a patient formally diagnosed with type II diabetes (PromoCell) were cultured in Endothelial Cell Growth Medium MV2 (EMV-2) supplemented with Growth Medium MV2 supplement pack (PromoCell) and antimycotic (Gibco). ..

    Article Title: A Versatile Microfluidic Extrusion-Based Hydrogel Platform for Self-Organization and Long-Term Maintenance of Engineered 3D Lymphatic Endothelium.
    Article Snippet: .. Materials andMethods .1 Cell Culture rimary human dermal lymphatic endothelial cells (HDLECs; romoCell, C-12216) and human dermal microvascular endotheial cells (HDMECs; PromoCell, C-12212) were cultured in ndothelial Cell Growth Medium MV2 (EGM2-MV; PromoCell, -22022). .. Human umbilical vein endothelial cells (HUVECs; onza, CC-2519) were cultured in Endothelial Cell Growth edium 2 (EGM2; PromoCell, C-22011).

    Article Title: Kynurenic acid mediates epicardial fat-induced lymphatic metabolic dysfunction in atrial fibrillation.
    Article Snippet: .. LECs, cardiac fibroblasts, and cardiomyocytes were cultured using Endothelial Cell Growth medium MV2 (Promo Cell, Catalog no. C-22022) or Cardiac Fibroblast Growth Medium-3 (Lonza, Catalog no. CC-4526) or Dulbecco’s modified Eagle’s medium (DMEM). ..

    Purification:

    Article Title: Endothelin-1 signaling regulates chamber-specific mouse atrial cardiomyocyte cytokinesis and polyploidy
    Article Snippet: .. Fibroblasts were collected from the remaining cells by pre-plating on 10 cm Petri dishes for 1 h. Purified endothelial cells were cultured in endothelial cell growth medium MV2 (Promocell, #C-22022) and maintained in 10 cm Petri dishes (Sarstedt, 83.3902) coated with 1% gelatin (Sigma, G1890). ..

    other:

    Article Title: Plasmin, the product of tissue plasminogen activator (tPA) treatment for ischemic stroke, impairs human brain endothelial barrier integrity
    Article Snippet: AIM-V serum-free media was prepared by supplementing AIM-V media (ThermoFisher Scientific, 12055091) with 1X GlutaMAX (ThermoFisher Scientific, 35050061) and 2MV supplements (PromoCell, C-39221 with FCS omitted) and stored at −80°C.

    Article Title: A single-nucleotide change in the Kozak sequence enhances protein expression
    Article Snippet: PromoCell Endothelial Cell Growth Medium MV2, WST-1 cell viability reagent, and ampicillin (sodium salt; A8351) were purchased from Merck KGaA (Darmstadt, Germany).

    Modification:

    Article Title: Kynurenic acid mediates epicardial fat-induced lymphatic metabolic dysfunction in atrial fibrillation.
    Article Snippet: .. LECs, cardiac fibroblasts, and cardiomyocytes were cultured using Endothelial Cell Growth medium MV2 (Promo Cell, Catalog no. C-22022) or Cardiac Fibroblast Growth Medium-3 (Lonza, Catalog no. CC-4526) or Dulbecco’s modified Eagle’s medium (DMEM). ..



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    <t>NK</t> <t>cell</t> characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.
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    Image Search Results


    Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate epithelial cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.

    Journal: Life

    Article Title: Effect of Butein, a Plant Polyphenol, on Apoptosis and Necroptosis of Prostate Cancer Cells in 2D and 3D Cultures

    doi: 10.3390/life15060836

    Figure Lengend Snippet: Cell viability, cell morphology, and nuclear morphology of butein against prostate cancer cell lines. ( A ) Chemical structure of butein. Formula: C 15 H 12 O 5 ; MW: 272.3. PC-3, DU145, and HPrEC cells were treated with butein at various concentrations (0, 1.25, 2.5, 5, 10, 15, 20, and 30 μM) and then cultured for 48 h. Viable cells were subjected to ( B ) trypan blue exclusion assay, and ( C ) MTT assay. ( D ) Cell morphology of PC-3 and DU145 cells was examined by phase contrast microscopy. ( E ) Nuclear morphology of PC-3 and DU145 cells was examined by fluorescence microscopy after DAPI (2 μg/mL) staining. All data are expressed as mean ± standard error of the mean of three independent experiments. *, p < 0.05 was compared to respective controls. HPrEC: human prostate epithelial cell line; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; and Conc., concentration.

    Article Snippet: HPrEC cells were cultured in Prostate Epithelial Cell Base Medium (ATCC, PCS-440-030) supplemented with (ATCC, PCS-440-040).

    Techniques: Cell Culture, Trypan Blue Exclusion Assay, MTT Assay, Microscopy, Fluorescence, Staining, Concentration Assay

    NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Journal: Journal of Extracellular Biology

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    doi: 10.1002/jex2.70049

    Figure Lengend Snippet: NK cell characteristics after different serum‐free culture conditions. (A) Workflow for static and shaking NK‐92 cultures, and purification of EVs. (B) Viability of NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions supplemented with IL‐15 was assessed by propidium iodide by flow cytometry. Control samples were NK‐92 cells grown in Advanced RPMI medium with 20% FBS. Data are presented as the mean ± SD of four separate experiments. Statistical analysis performed using the Friedman one‐way ANOVA test indicated no statistical difference between the different culture conditions. (C) Expression levels of CD56 on NK‐92 cells after 48 h of culture in serum‐free media under static or shaking conditions. One representative experiment of three.

    Article Snippet: NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF).

    Techniques: Purification, Flow Cytometry, Control, Expressing

    TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Journal: Journal of Extracellular Biology

    Article Title: Evaluating the Influence of Different Serum‐Free Culture Conditions on the Production and Function of Natural Killer Cell‐Derived Extracellular Vesicles

    doi: 10.1002/jex2.70049

    Figure Lengend Snippet: TEM images of purified EVs derived from different serum‐free NK cell culture media. (A) TEM images of EVs obtained from static culture conditions. (B) TEM images of EVs obtained from shaking culture conditions. Scale bar 200 nm. Images are representative of 8–10 images from each sample.

    Article Snippet: NK‐92 cells (5 × 10 7 cells) were resuspended in 65 mL of serum‐free media with 10 ng/mL of recombinant human IL‐15 (R&D Systems) and cultured for 48 h. Six different serum‐free media optimized for culturing of NK cells were tested: CellGenix GMP SCGM (xeno‐free, Sartorius [abbreviated GMP SCGM]), CTS NK‐Xpander Medium (xeno‐free, ThermoFisher [abbreviated CTS]), Immunocult NK Cell Base Medium (xeno‐free, StemCell Technologies, abbreviated Immunocult), ExCellerate Human NK Cell Expansion Media, Xeno‐Free (R&D Systems, abbreviated ExCellerate XF), and ExCellerate Human NK Cell Expansion Media, Animal Component Free (R&D Systems, abbreviated ExCellerate ACF).

    Techniques: Purification, Derivative Assay, Cell Culture